Team:Paris Bettencourt/jb

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

• Background
• Design
• Project
  Micronutrients
  Continuity
  Modeling
  Manufacturing
• Achievements
  Results
  Parts
  Medal Fulfillments
• Practices
  Overview
  SynBio Challenge
  DIλ
  Acceptance
  Events
  Rhizi
  Collaborations
• Safety
  Safety Overview
  Safety Form
  Review Board
• Notebook
  Daily notebook
  Protocols
  Bibliography
• Team
  Members
  Attributions
  Acknowledgments
  Press
  Official Team Profile

Notebook

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

o15.053	HO-Poly-KanMX4-HO amplification (reverse) + tail	TGAAATGGCGAGTATTGATAATGATAAACTCTGTCAAGGAGGGTATTCTGGGC
o15.056	gBlock2 amplification (forward) + tail	TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC
o15.057	gBlock2 amplification (reverse) + tail	GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT
o15.058	gBlock3 amplification (forward)	AACGTTATTGTTCGGTCCTCCATTGC
o15.059	gBlock3 amplification (reverse) + tail	GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG
o15.060	gBlock4 amplification (forward)	CTTGAGGCTACAATTCATTGGTCAAATAC
o15.061	gBlock4 amplification (reverse) + tail	TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC
o15.117	forward primer for gblock 1 part 1	CGTCCAAGACATACTGCGTTTACG
o15.118	reverse primer for gblock 1 part 1	GTGTGCAACATTCCCACTACATTTTGAATG
o15.119	forward primer for gblock 1 part 2	CATTCAAAATGTAGTGGGAATGTTGCAC
o15.120	reverse primer for gblock 1 part 2	TGGATTGTTGGGAAGGAAAACTTTCCTT
o15.121	forward primer gblock 1 + restriction site ecor1	TATACTGCAGTCTAGAACTAGTGGATCCATGGATTAC
o15.122	reverse primer gblock 1 + tail	AGCCTGGAGGAAGGGTTAGCATGGATGGAATGGATTGTTGGGAAGGAAAACTTTCCTT
o15.123	forward primer TDH3 from registry with HO binding tail	GCCCAGAATACCCTCCTTGACAGACAGTTTATTCCTGGCATCCACTAAATATAATG
o15.124	reverse primer TDH3 from registry with RE binding site	TATACTGCAGTTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC
o15.125	forward primer full gblock 1 with new promoter	GCCCAGAATACCCTCCTTGACAG
o15.126	reverse primer full gblock 1 with new promoter	CATGCTAACCCTTCCTCCAGGCT
o15.127	forward primer gblock 2 + tail	TCACAACAAGAATTTTGCAGAGGACCTAACCGAAGGAAAGTTTTCCTTCCCAACAATC
o15.128	reverse primer gblock 2 + tail	GGACTTGATAGAATTTTGTCTGGTTGACTCGGATGCAATGGAGGACCGAACAAT
o15.129	forward primer gblock 3	AACGTTATTGTTCGGTCCTCCATTGC
o15.130	reverse primer gblock 3 + tail	GTCCAGATTGACTCGAATGGGTGTAATGCCAGTATTTGACCAATGAATTGTAGCCTCAAG
o15.131	forward primer gblock 4	CTTGAGGCTACAATTCATTGGTCAAATAC
o15.132	reverse primer gblock 4 HO tail	TGTACGGGCGACAGTCACATCATGCCCCTGCAGGGTACCGCGAATTCCCC
o15.133	reverse primer gblock 4 HMG tail	CATGCGCCTATTATTACTTCAATAGATGGCAGGGTACCGCGAATTCCCC
o15.134	forward primer vector HO with HMG	GCTTTAATTTGCGGCCGGTACCCAATTCGCCAGGGGCATGATGTGACTGTCG
o15.135	forward primer vector HO without HMG (binds to gBlock4)	TCGCTATACTGGGGAATTCGCGGTACCCTGCAGGGGCATGATGTGACTGTCG
o15.136	reverse primer vector HO (binds to TDH3?)	TATATTTAGTGGATGCCAGGAATAAACTGTCTGTCAAGGAGGGTATTCTGGGC
o15.137	formard primer HMG+tail with RE	TATATCTAGACCCGCCGCCACCATGCAAAG
o15.138	reverse primer HMG+tail with restriction site	TATACTCGAGTCACTGTTGAGACCTCAAATCCTGTAAG
o15.139	forward primer promoter HMG terminator	TCGCTATACTGGGGAATTCGCGGTACCCTGCCATCTATTGAAGTAATAATAGGCGCATG
o15.140	reverse primer HMG + tail HO	TGTACGGGCGACAGTCACATCATGCCCCTGGCGAATTGGGTACCGGCCG
o15.141	reverse primer gblock 1 part 1 (binds to part2))	TCCTCAACGTCGTCCATCAGTAAGCTCGCTGTGTGCAACATTCCCACTACATTTTGAATG
o15.142	forward primer gblock 1 + tail that binds to HO pl	GCCCAGAATACCCTCCTTGACAGCGTCCAAGACATACTGCGTTTACG
o15.143	reverse primer HO + tail crappy ADH1	TCGACACGTAAACGCAGTATGTCTTGGACGCTGTCAAGGAGGGTATTCTGGGC
o15.144	forward primer gblock 1 + tail TDH3	GTTTCGAATAAACACACATAAACAAACAAATCTAGAACTAGTGGATCCATGGATTAC
o15.145	reverse primer TDH3 + tail for kozac	TCCATGGATCCACTAGTTCTAGATTTGTTTGTTTATGTGTGTTTATTCGAAACTAAGTTC