Team:Paris Bettencourt/Protocols/Digestion
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
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Vitamin A
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E. coli
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S. cerevisiae
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Idli and Micro-organisms
Digestion Protocol
Prepare the following mix:
4μL of Enzyme 1
4μL of Enzyme 2
4μL of FastAP
12μL of Fast Digest buffer 10X
1 to 3 μg of DNA
up to 120μL of water
mix by pipetting up and down
incube 10min at 37°C