Team:Paris Bettencourt/Protocols/Annealing

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

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Notebook

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

Annealing Protocol Phosphorylation of the oligos 5.6μL DNAse/RNAse free water 6.0μL o15.011 (10µM) 6.0μL o15.012 (10µM) 2.0μL 10X T4 DNA ligase buffer 0.4μL T4 PolyNucleotide Kinase Total: 20μL incube 30min at 37°C add 1μL of 1M NaCl incube 5min at 95°C let the mix cool down use 2μL of the mix as a 10X solution