Team:Paris Bettencourt/Project/VitaminB2

Background

Aims

Results

Some microorganisms (e.g. B. subtilis) naturally produce riboflavin in high amount. Make Idli batter microorganisms produce high amount of riboflavin during fermentation to increase the vitamin B2 content of the batter.
  • Successfully cloned the full riboflavin biosynthesis pathway of B. subtilis in E. coli and made it over-produce vitamin B2 and proceeded to chromosomaly integrate the full pathway in Lactococcus lactis
  • BioBrick submitted to the BioBrick Registry: BBa_K1678001, BBa_K1678002, BBa_K1678003, BBa_K1678004