Team:Paris Bettencourt/Protocols/titration-acid-phytic

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

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Notebook

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

Titration of phytc acid using Megazyme© Kit Preparation of reagent solutions (not supllied) : Trichloroacetic acid (50% w/v) : 100mL 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)