Team:Paris Bettencourt/Protocols/titration-acid-phytic
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
- Background
- Design
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Vitamin A Vitamin B2 Vitamin B12 Phytase Riboswitch Differentiation on E. coli Differentiation on S. cerevisiae Manufacturing Idli and Micro-organisms Titration of phytc acid using Megazyme© Kit
- Preparation of reagent solutions (not supllied) :
- Trichloroacetic acid (50% w/v) : 100mL Add 50g of thrichloroacetic to 60mL of distilled water and dissolve. Make to volume (100mL) with distilled water. (Stable for > 6 moths at 4°C)
- Hydrochloric acid (0.66M) : 1L Add 54.5mL of hydrochloric acid to 945.5mL of distilled wtaer an mix. (Store at room temperature)
- Sodium hydroxide (0.75M) : 200mL Add 6g of sodium hydroxide pellets to 180mL of distilled water and dissolve. Make to volume (200mL) with distilled water. (Store at room temperature)
- Phytic acid : Pure phytic acid control sample may be required.
- Sample extraction :
- Weigh 1g of sample material.
- Add 20mL of hydrochloric acid (0.66M).
- Cover with foil and incubate for a minimum of 3 hours at room temperature.
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