Team:Paris Bettencourt/Protocols/Gel Electrophoresis

Gel-Electrophoresis protocol
  • Preparation of the Gel (50mL)
    • Weight 50mg of Agarose NEEO.
    • put the 50mg of Agarose in an Erlenmeyer and add 50mL of TAE 0.5%.
    • Heat the Erlenmeyer in a microwave until the liquid becomes totally transparent.
    • Let it rest until you can take the Erlenmeyer in your hand without pain for 10 seconds.
    • Add 5uL of SYBR® Safe and mix gently until the liquid takes and uniform reddish color.
    • Pour the liquid in a gel mold with the desired number of well and wait 20-30 min (or until the gel is solidified).
    • Put the gel inside the electrophoresis machine and pour some TAE 0.5% until the gel is totally submerged.
  • Preparation of the samples
    • Mix 5uL of each sample with 1uL of 6X loading dye in microcentrifuge tubes/ on a piece of parafilm / in PCR tubes.
  • Pipette 5-6uL of each sample inside a different well.
  • Pipette 5uL of the appropriate ladder in one of the wells.
  • Close the machine and launch the migration with the desired voltage
  • After the desired migration time turn of the machine, remove the lid and put the gel inside the Blue light revelator.