Received Propionibacterium freudenreichii subsp. shermanii CIRM BIA1 from the INRA Rennes CIRM collection.
Made the following media:
MEA (waking lyophilized bacteria up), 1 L
1
Product
Quantity
BHI broth
37 g
Soja peptone
5 g
Yeast extract
5 g
Glucose
3 g
Water
Fill to 1 L
YEL (propionibacteria growth medium), 1 L
References
Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.
B12 measurement: http://iioab.org/Vol2%282%292011/Karmi%20et%20al-IIOABJ-2%20%282%29-2011-23-32p.pdf
