Team:Paris Bettencourt/Notebook/VitaminB12

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

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Notebook

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

• July
• August
• September

July

July 28th

• Received Propionibacterium freudenreichii subsp. shermanii CIRM BIA1 from the INRA Rennes CIRM collection.
• Made the following media:
  • MEA (waking lyophilized bacteria up), 1 L

    Product	Quantity
    BHI broth	37 g
    Soja peptone	5 g
    Yeast extract	5 g
    Glucose	3 g
    Water	Fill to 1 L

  • YEL (propionibacteria growth medium), 1 L

    Product	Quantity
    Sodium lactate (60% syrup)	21.4 g
    Tryptone	10 g
    Yeast extract	10 g
    K2HPO4, 3 H2O	328 mg
    MnSO4, H2O	56 mg
    Water	Fill to 1 L

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References

Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.
B12 measurement: http://iioab.org/Vol2%282%292011/Karmi%20et%20al-IIOABJ-2%20%282%29-2011-23-32p.pdf