Ferment It Yourself

iGEM Paris-Bettencourt 2O15

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

July
August
September

July

July 28th

Received Propionibacterium freudenreichii subsp. shermanii CIRM BIA1 from the INRA Rennes CIRM collection.
Made the following media:
- MEA (waking lyophilized bacteria up), 1 L
  
  Product Quantity
  
  BHI broth 37 g
  
  Soja peptone 5 g
  
  Yeast extract 5 g
  
  Glucose 3 g
  
  Water Fill to 1 L
- YEL (propionibacteria growth medium), 1 L
  
  Product Quantity
  
  Sodium lactate (60% syrup) 21.4 g
  
  Tryptone 10 g
  
  Yeast extract 10 g
  
  K₂HPO₄, 3 H₂O 328 mg
  
  MnSO₄, H₂O 56 mg
  
  Water Fill to 1 L
Successfully grew P. freudenreichii in MEA at 30°C, in aerobic conditions.

July 30th

Tested the YEL+agar medium with the following strains:
- P. freudenreichii
- Lactobacillus lactis
- E. coli
- Lactobacillus plantarum
- Saccharomyces cerevisiae
Result (after 24 hours):

References

Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J. H., Deery, E., ... & Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Microbial biotechnology, 3(1), 24-37.
B12 measurement: http://iioab.org/Vol2%282%292011/Karmi%20et%20al-IIOABJ-2%20%282%29-2011-23-32p.pdf

Team:Paris Bettencourt/Notebook/VitaminB12