Team:Paris Bettencourt/Sustainability/Continuity

When going from labs lead by specialists to the users community, a lot of technical challenges arise. When inventing new biotechnological devices, biologists have access to biosafety cabinets, powerful freezers and autoclaves, but the people who need our product the most won't have these. For a biological product to leave the benches and actually reach the population, it's essential to foresee its life in the hands of the people who will cultivate it and make sure it stays alive all along. Here, we provide strategies to create an durable, usable product.

Specification

From the lab to the real world

Mutations and loss of pathways

Vanderbilt collaboration Chromosome integration

Make it extend

decoupling [prototype] limit R&D expenses

All in one

Our design

Overview

[construction] [growth]

The chassis

[chassis]

Orthogonal recombination sites

Allowing for quality control

Landing pads

Differentiation

how to induce it

Results

Integration in bacterial cells

assembly fluorescent proteins are present sequence is correct

Function of the promoter

Orthogonality of the LoxP sites

Induction of the differentiation

Effects on growth

Outlook

link