Team:Paris Bettencourt/Sustainability/Continuity
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
- Background
- Design
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Notebook
Vitamin A Vitamin B2 Vitamin B12 Phytase Riboswitch Differentiation on E. coli Differentiation on S. cerevisiae Manufacturing Idli and Micro-organisms When going from labs lead by specialists to the users community, a lot of technical challenges arise. When inventing new biotechnological devices, biologists have access to biosafety cabinets, powerful freezers and autoclaves, but the people who need our product the most won't have these. For a biological product to leave the benches and actually reach the population, it's essential to foresee its life in the hands of the people who will cultivate it and make sure it stays alive all along. Here, we provide strategies to create an durable, usable product.
Specification
From the lab to the real world
Mutations and loss of pathways
Vanderbilt collaboration Chromosome integration
Make it extend
decoupling [prototype] limit R&D expenses
All in one
Our design
Overview
[construction] [growth]
The chassis
[chassis]
Orthogonal recombination sites
Allowing for quality control
Landing pads
Differentiation
how to induce it
Results
Integration in bacterial cells
assembly fluorescent proteins are present sequence is correct
Function of the promoter
Orthogonality of the LoxP sites
Induction of the differentiation
Effects on growth
Outlook
link