Team:Aachen/Attributions

The Challenge

The Team

The Strategy

Biological Approach

The Bioreactor

The People

Maker Faire

Community Labs

tubefront

The Solution

Engineering

Biology

The Future

Members

Teamwork occurs when diverse abilities and insights join together to work toward a common goal. Therefore, students of different age, gender and field of study came together to found our team in Aachen. During the project we not only had the chance to learn from each other, but also to excel ourselves. So all in all with joint forces and collective creativity, we will create something special in order to make our contribution to synthetic biology.

Sarah Lubjuhn

Sarah Lubjuhn

"xxx"

Molecular and Applied Biotechnology (B.Sc. student)

only smiles less when she gets tired, sunny disposition

After completing the third semester in Biotechnology at the RWTH Aachen, I decided to take a semester off to participate in the iGEM competition. I heard about iGEM in a lecture and found the aspects of synthetic biology and the possibility to be part of a self-managed, independent student reasearch team very appealing. Looking back, I really learned a lot, not only about labwork and designing experiments, but also about teamwork and communicating synthetic biology to the public. All in all it was a great semester and although it was stressful at times, I really enjoyed being part of the iGEM competition.

responsible	involved	helping
Glycogen Cloning	"in silico" Design	Promoter Library
Glycogen Analytics		Public Relations

Sven Kratel

Sven Kratel

"xxx"

Molecular and Applied Biotechnology (M.Sc. student)

grumpy Sven, hero of wikistructur and desing, free-spoken realist

xxx

responsible	involved	helping
Modeling	Promoter Library	Methanol Cloning
Wiki Coding		Glycogen Cloning
Design

Tobias Schwanemann

Tobias Schwanemann

"Der Doppelpipetator" ("The Doublepipetor")

Molecular and Applied Biotechnology (B.Sc. student)

extremly sportif, has a lot of energy

I participated in the iGEM competition to get new experiences in the field of sythetic biology because it is a very fascinating and exciting topic in my studies for me. Therefore, I took a study semester off to concentrate completely on the new challenge. During the iGEM project I have learned to design several experiments and to do different kinds of work in the lab. I also gained experiences in presenting a project to an audience and countless more. These experiences will influence my future academic studies. In my spare time I do gymnastics and I have successfully been doing Cyr Wheel for 2 years winning the silver medal at the world championships 2015.

responsible	involved	helping
Promoter Library	Experiment design (MDH-Characterization)	in silico Design
Methanol Analytics		Methanol Cloning

Martin Böhne

Martin Böhne

"xxx"

Biology (M.Sc. student)

Besides my master's studies in Biology at RWTH Aachen University I wanted to learn more about project management and working in a self-organizing team of students. The iGEM competition and the iGEM Team Aachen are the right opportunity to seize it. Learning more about the experiment design and data analysis was great. It was also a great experience to see and get the support of the people from the different institutes, which were not directly involved in our iGEM project. In my free time I like to go swimming and participate in debates at the university's debating club (the latter happens to seldom due to iGEM and my studies, unfortunately).

responsible	involved
Glycogen Analytics	Safety

Tanya Bafna

Tanya Bafna

"Pikachu"

Biomedical Engineering (M.Sc. student)

xxx

responsible	involved
Circuit Design	Bioreactor Characterization
Hardware Design
Microcontroller Firmware

Sebastian Siegel

Sebastian Siegel

"xxx"

Electrical Engineering (B.Sc. student)

Communication geek

The studies of electrical engineering are quiet theoretical, so for a long time I wanted to do something practical. Than I came across iGEM competition from a neighbour and was fascinated about the possibility to do some practical work in a scientific context and, even more, think outside the box of my subject and explore things far beyond. But fast I realized that there are many connections between biology and electrical engineering, problems that none of the subjects can solve on its own but that we will work on together. And therefor it is common opportunities. This is what makes iGEM such a great matter to me.

responsible	involved	helping
Hardware design	Fundraising
Microcontroller Firmware	Bioreactor Control Software

Laura Grabowski

Laura Grabowski

"Grebauski"

Molecular and Applied Biotechnology (B.Sc. student)

utility woman

After completing my third semester I wanted to get into the lab and use Synthetic Biology to build something new. For that reason, I dedicated my entire summer term to our iGEM project. Over the past few months I was able to contribute to a diverse field of work and enjoyed working in a team of complementary strengths. I particularly enjoyed the exchange with other iGEMers and supporters. Besides my studies I am interested in cooking and city travels.

responsible	involved	helping
Promotor Library	Glycogen Analytics	Public Relations
	MethanolAanalytics

Lisa Hannappel

Lisa Hannappel

"xxx"

Molecular and Applied Biotechnology (M.Sc. student)

I completed my undergrad degree in biotechnology at FH Aachen University and wrote my bachelor thesis about: "Characterization of an anti-Muc1 chimeric antigen receptor (CAR)" at the Center for Molecular Medicine Cologne. For me participating in the iGEM is a great opportunity to look beyond the horizon. To handle our own research project we have to take various tasks into consideration: fundraising, labwork, public relations and many others. For me this complexity made iGEM so exciting! In my free time I love spending time with my friends and sailing at the Rursee.

responsible	involved	helping
iGEM Celebration	Fundraising	Filmproject
		Safety

Patrick Opdensteinen

Patrick Opdensteinen

"designi heini"

Molecular and Applied Biotechnology (M.Sc. student)

loves to tidy up, you can translate my last name to “on-the-rocks” :)

This is my second year within the iGEM community. I am still fascinated by synthetic biology and the possibility to realize a research project from scratch to finalization within an interdisciplinary team. Based on the experience gained through our first participation in the iGEM competition I wanted to help this year´s team from Aachen to perform even better than our first team and to prevent some of the mistakes we did last year. Besides participating in iGEM I work as a student assistant at the RWTH Aachen´s Institute for Molecular Biotechnology (BIO VII). In my student assistant job I am mainly involved in the purification of plant made biopharmaceuticals from tobacco. In my spare time I like to do sports such as Volleyball and Badminton.

responsible	involved
3D printing	Fundraising
Design

Tobias Pickert

Tobias Pickert

"Pickasso"

B.Sc. Chemistry

Fascinated by natural sciences I participated in different international science competitions during my school time because spreading innovative ideas to the public always seemed important to me in case of advancing solutions for challanges of our society. My enthusiasm of natural sciences, the chance to improve my soft skills and learn new scientific hard skills within the great atmosphere of an interdisciplinary student team have mainly motivated me to participate in the iGEM competition 2015. In my opinion my iGEM experiences have offered me interesting insights in biochemistry, synthetic biology and correlated lab work that I would not have learned in my chemical studies. The combination of exciting wetlab experience, weekly English team meetings, the possibility to creatively get involved in diverse sub topics e.g. fundraising, design tasks or public relations is shortly summarized that was iGEM is about based on my experiences. These key possibilities together with my great team members have resulted in one year of hard work as well as pleasure to me. I am sure that these experiences are going to support my future work as a chemist. As a balance to my research work I like to spend time with my family and go on bicycle or jogging adventures with friends in nature.

	involved	helping
	Fundraising	Design
	Glycogen Analytics	Community Labs

Michael Osthege

Michael Osthege

"cake"

Molecular and Applied Biotechnology (M.Sc. student)

When last years iGEM competition was over, I decided to take part for a second time before proceeding with my master studies. In 2014 we had done some mistakes when it came to project management and learned a lot. So early into this years project, I focused on our internal infrastructure and became responsible for teaching our new team members what's important in iGEM and how synthetic biology works in the lab.

responsible	involved	helping
Bioreactor Control Software	Project Coordination	Design
Internal Infrastructure	Modeling	Glycogen Analytics
Tubefront	Data Analysis
	Glycogen Cloning
	Microcontroller Firmware

Stefan Backhaus

Stefan Backhaus

"xxx"

Biology (M.Sc. student)

During my studies I realized that I like to challenge myself and dig into new topics. Therefore I took a varity of course and challenged myself to get an understanding in the different fields of biology, chemistry, physics and informatics. I soon found interdisciplinary sciences to combine the different topics to create incredible solutions for the problems of our time. Until my first contact with the synthetic biology, I worked in biomimetics, taking courses, programming genetic algorithms and even doing my bachelor thesis in this field. When I heard about the iGEM project, I participated because it is not only interdisciplinary, but also allows students to develope their own project. Our Instructor and Advisor are always willing to answer our questions but also give us the freedom to work indepentdently on our project. Therefore, everyone makes incredible valuable experiences. I personally focused on coordinations and collaborations, learning not only about synthetich biology but also about important softskills.

responsible	involved	helping
Public Relations	Project Coordination	Design
Finances & Administration	Fundraising
Film Project
Community Labs

Philipp Demling

Philipp Demling

"Jonny Nash"

Molecular and Applied Biotechnology (M.Sc. student)

HPLC-god, has a second family in US, can stamp out wells from a microtiterplate

xxx

responsible	involved	helping
Methanol Analytics	Fundraising	Project Coordination
	Finances & Administration

Laura Helleckes

Laura Helleckes

"xxx"

Molecular and Applied Biotechnology (B.Sc. student)

knows how to destroy a test tube with a plastic tip, our moviestar, golf and convertible, notes down when you mock someone ;)

My name is Laura and I major in Molecular and Advanced Biotechnology at RWTH Aachen. During my third semester I learned about iGEM and was fascinated by the possibility to plan and execute an independent scientific project in the early phase of my studies. Therefore, I took a semester off to join the team Aachen. For me the project was a great opportunity to share my ideas with other motivated students, to profit from their diverse abilities and to have a great summer. By now I can say that I learned a lot about designing experiments and working in the lab, but also about organizing a team and how to present synthetic biology to the public. In my free time, I like to do sports, especially playing golf. Apart from that, I enjoy reading a thrilling book or cooking with friends.

responsible	involved	helping
Glycogen Cloning	"in silico" Design	Modelling
Glycogen Analytics	Data Analysis	Public Relations

René Hanke

René Hanke

"xxx"

Molecular and Applied Biotechnology (M.Sc. student)

nice and crazy, the experienced one

Last year I did my Bachelor Thesis at the "Institute of Applied Microbiology" (iAMB) in "Developing an E. coli expression system for high throughput analysis". The same year, we founded and run the first iGEM Team of Aachen with the project "Cellock Holmes - A Case of Identity". In October I will be starting the 2. year of my master studies of Molecular and Applied Biotechnology. The iGEM experience 2014 was so overwhelming that I had to be part of it once more! I felt obliged to help the next team, but got so excited about the new project and team that I became a member again. The opportunity to manage such a huge and diverse project in combination with the work in an multinational, interdisciplinary team is what fascinates me the most. Similar to last year, I mainly focus on communication, presentation and coordination.Apart from the iGEM work, I like to ride my bike, enjoy a nice match of Tennis and love to go dancing with my girlfriend.

responsible	involved	helping
Film Project	Fundraising	Finances & Administration
Community Labs	Project Coordination	iGEM Celebration
Public Relations

Carolin Grütering

Carolin Grütering

"The Destroyer"

Molecular and Applied Biotechnology (B.Sc. student)

After three semesters of theoretical studies of Biotechnology in Aachen, it was perfectly time to gain some practical experiences for me. Thus, having a great summer with an iGEM project, together with many motivated students was the perfect opportunity. It sounds like a cliché, but I learned much more in this very intense time than I ever expected. It was awesome to experience team work in an interdisciplinary group and to get in contact with iGEM members from other towns. When I'm not doing iGEM stuff, I do sports, make cakes, dance and meet my friends outside the laboratory.

responsible	involved	helping
Methanol Cloning	Methanol Analytics	Promoter Library
	"in silico" Design

Jonas Rührer

Jonas Rührer

"sneaky pen thief"

Molecular and Applied Biotechnology (B.Sc. student)

king of nightshifts

Before participating in iGEM I've been studying Molecular Biotechnology for three semesters. iGEM is a great opportunity for me to get a first insight in scientific work.

responsible	involved	helping
Methanol Cloning	Methanol Analytics	Promoter Library
	"in silico" Design	Modelling

Sayantan Dutta

Sayantan Dutta

"Santana Bollywood, Satan"

Biomedical Engineering (M.Sc. student)

our indian workaholic, ladiesman

Being a student of Electronics and Instrumentation from India, I came here to Germany to study Biomedical Engineering because of my facination towards medical and biological instruments. During my Bioinformatics Lecture one day I was introduced to the plan and the idea of iGEM. The one thing that made me join this team was the fact that I could use my own ideas and work on Biological Instruments. This great Opertunity helped me develop hardware and software. Learning and working with theories and plans outside the box is what I am good at because I have always been involved in practical hardware projects. Hardware circuit designing and interfacing with software and arduino firmware is always complicated, this makes it much more fun to integrate and compensate the electronics by burning or in technical terms "overheating" the system. While building this Bioreactor I learned that sometimes burning circuits helps you to understand which is the correct way and which is not.

responsible	involved	helping
Circuit Design	Bioreactor Characterization	Public Relations
Hardware Design		Bioreactor Control Software
Microcontroller Firmware

Marc-Daniel Hagel

Marc-Daniel Hagel

"Tomatenmark"

Biology (M.Sc. student)

Before coming in contact with synthetic biology i was mainly interested in the field of molecular medicine in which I wrote my bachelor thesis and mained my master courses. After finishing my third master semester I thought for a challenge. The idea of participating in the creation of a whole project with pepole whom i did not know intrigued me. To see every aspect of such a project from the design of the molecular approach combined with projectmanagement to fundraising and communication to the outerworld unfold right before my eyes, not to underestimate the experience with such amazing people will let me remember this time forever. I am grateful for this experience! My hobbys involve photography, cooking and being a movie junkie.

responsible	involved	helping
Glycogen Analytics	Design	Film project
		"in silico" Design

Advisors

Dr. rer.nat. Ljubica Vojcic

Dr. rer.nat. Ljubica Vojcic

I am working as a Subgroup Leader in Prof. Schwaneberg research group at the Institute of Biotechnology, RWTH Aachen. The research area of the Schwaneberg group focuses on directed protein evolution by developing novel methods for generating diversity at the gene level, analyzing consequences of mutational biases on the protein level and developing novel high-throughput screening systems that will lead to improved biocatalysts for prominent applications in industry.

In particular, my core expertise is development of high throughput screening systems for different enzyme classes in order to redefine the screening step no longer as bottleneck in directed evolution.In iGEM, I enjoyed very much to work with highly motivated and ambitious young scientists from RWTH Aachen University. I truly believe that our collaboration has just started and that we will enjoy jointly solving the scientific challenges in the near future.

Dr. Suresh Sudarsan

Dr. Suresh Sudarsan

I joined the lab of Prof. Lars Blank at RWTH Aachen University in September 2012, for my post-doctoral research on investigating the glycerol metabolism in E. coli for bio-product synthesis. During my Ph.D, I worked under the guidance of Prof. Andreas Schmid (2008-2012) at TU Dortmund, and Prof. Matthias Reuss (2009-2011) at the University of Stuttgart, with a focus on elucidating the link between the central and aromatic metabolism of P. putida using a systems biology approach.

I'm fascinated about understanding the collective dynamic behavior of microorganisms and their metabolic potential in different niches. Hence to decipher the metabolic dynamics, I use the tools of metabolic engineering & systems biology such as metabolomics, fast sampling, kinetic/dynamic modeling and metabolic flux analysis.In iGEM, I enjoy working with a group of young scientists from Aachen to achieve our common goal enabling bacteria to grow on methanol and accumulate glycogen. Besides science, I try to get myself involved in adventurous sports...but honestly... I just like to relax my day with a delicious meal and a good sci-fi movie! ;-)

Instructors

Prof. rer.nat. Ulrich Schwaneberg

Prof. rer.nat. Ulrich Schwaneberg

RWTH Institute for Biotechnology, Leibniz Institute for Interactive Materials (DWI)

The Schwaneberg Group seeks to be at the research frontier in the interdisciplinary field of directed protein evolution by developing novel methods for generating diversity at the gene level, analyzing consequence of mutational biases on the protein level and developing novel high-throughput screening systems that will ultimately lead to tailored-biocatalysts for significant applications in industry.

They train students in the cutting edge technologies of laboratory evolution,biocatalyst engineering and high throughput screening methodologies. The Schwaneberg Group believes in integrating fundamental principles of protein design with environmental awareness in their research and seeks to promote international scientific collaborations. Read more about Prof. Schwaneberg and his work on the Schwaneberg Group's website.

Prof. Dr. rer.nat. Wolfgang Wiechert

Prof. Dr. rer.nat. Wolfgang Wiechert

Forschungszentrum Jülich, Institute of Bio- and Geosciences (IGB-1)

Prof. Wiechert's main area of work lies within the field of applied systems bio(techno)logy of microorganisms with a special focus on methodological developments for quantitative biology. Characteristics of his research work are a close integration of experimental and theoretical work within multi disciplinary projects. As the head of the Systems Biotechnology research division at Forschungszentrum Jülich, he is developing methods for quantitative metabolomics, fluxomics and proteomics including model based mathematical methods for experimental design, parameter estimation, and process optimization in biotechnological systems.

Future work will also incorporate micro fluidic methods for single cell analysis. In general, all research results are used to drive forward the process of gaining knowledge in the course of an iterative improvement of industrial production systems. This proceeds in close cooperation of all working groups at the IBG-1.Together with industrial partners also diverse examples from industry are investigated and further developed. Read more about Prof. Wiechert's work on the IBG-1 website.

Prof. Dr.-Ing. Lars Blank

Prof. Dr.-Ing. Lars Blank

RWTH Institute for Applied Microbiology (iAMB)

Prof. Blank focuses his research on fundamental and applied aspects of microbial metabolism. Of specific interest is the interaction between the metabolic network and the introduced genetic and environmental perturbations. The research on in silico/in vivo metabolic network operations is aimed at a deeper understanding of cell function, with the ultimate goal of rational cell engineering.

In his teaching, Prof. Blank focuses on the integration of biological concepts with the tools from bioinformatics and engineering. He believes that a sound knowledge base in life sciences is the key for creative and thus successful work in the areas of Metabolic Engineering and Synthetic Biology. Read more about Prof. Blank's work on the iAMB's website.

Partners

This project would not have been possible without the generous help and advice from many great people and organizations. We thank all the partners listed below for their essential contributions and financial support that covered registration fees, laboratory materials and expenses for services and materials.

Attributions

Scientific and General Support Great projects do not only depend on solid funding, but even more on the invaluable support by great people. We would like to thank all the great people for letting us use their lab, providing laboratory equipment and supporting our project in a different way.

• Exploratory Research Space
• Dr. Brigitte Küppers, for the opportunity to participate in Symposium

• Fab Lab Aachen
• Jan Thar, for support concerning Lasercutter, PCB Mill and 3D printing

• Forschungszentrum Jülich
• Dr. Hans-Peter Buchkremer, for
• Dr. Martin Finsterbusch, for the contact to sunfire
• Marianne Heß, who was essential for travel organization and Finances
• M.Sc. ETH Jannick Kappelmann, for support and supervision for labelng experiments
• Dr. Jan Marienhagen, for scientific information about the enzymes for MCC pathway
• Dr. Stephan Noack, for
• Prof. Martina Pohl, for
• Dr. Frank Tietz, for
• Sabrina Witthoff, for

• Fraunhofer IME
• Dr. Johannes Felix Buyel, for helpful feedback regarding initial project ideas
• Claudia Hansen, providing us with Fraunhofer Applied Genomics Sequencing Service
• Raphael Soeur, providing us with Applied Genomics Sequencing Service
• Dr.Holger Spiegel, gave us access to the 3D printing at the Fraunhofer IME

• HSP II
• Dr. Tamara Dworeck, for coordination of practical course lab
• Dr. Nicole Maaßen, for coordination of practical course lab

• Medlife
• Dr. Carin Jansen, for the contact to Storaenzo, her invitation to the summer party and joining of Technik Garage into network

• MSE
• Markus Meuresch, for

• proRWTH
• Jeannette Schwerdt, helping us with donation details

• RWTH Aachen Biology Department
• Dr. Sonja Gieseking, for support concerning Finances & Administration
• Dr. Monika Reiss, for Administration for iGEM Celebration and release of practical course documents
• Timur Toygar, for seminars on communication and project management

• RWTH Aachen Biology V
• Dr. Hanno Zielke, for establishing contacts with students and helping with videos

• RWTH Aachen Department 4.0 Research and Career
• Elke Müller, for

• RWTH Institute of Applied Microbiology (iAMB/Biology IV)
• Gisela Beissel, for
• Monika Brehm, for
• Henrik Cordes, for his feedback on our project
• Kalle Hüser, helping us with everything
• Prof. Dr. Jan Schirawski, providing lab space
• Ivan Schlembach, giving the Re-introduction to Microscropy at Blank Lab
• Ulrike Schmitt, who was resposinble for Finances at iAMB
• Anette Schreer, for
• Dr. Martin Zimmermann, providing lab space and for his general support

• RWTH Institute of Biotechnology
• M.Sc. Alexander Richard Grimm, general scientific support
• Dr. Stefanie Hamer, giving us competent cells of E. coli T7 Shuffle Express (DE3)
• Georgette Körfer, giving the FACS introduction at Schwaneberg Lab
• Andreas Nessel, for safety introduction and coordination of scientific work
• Dr. Anna Joelle Ruff, giving us competent cells of E. coli C43 (DE3)and explaining QuikChange

• RWTH Institute of Moleculare Biotechnology (Biology VII)
• Dr. Ulrich Commandeur, providing lab space and material
• Christina Dickmeis, for
• Karolin Richter, for
• Juliane Röder, for

• RWTH Press Office
• Celina Begolli, for
• Sebastian Dreher, for the contact to Wisam Zureik
• Angelika Hamacher, making an interview for RWTH inside

• RWTH Rectorate
• Prof. Dr. Krieg, giving an interview on iGEM and funding of filmproject
• Nicole Klinkenberg, for communication with Prof.Dr.rer.nat.Krieg

• Students
• Biotechnologische Studenteninitiative (btS), organizing BBQ
• Fachschaft Biowissenschaften, organizing BBQ, supplying food and for general support
• Andreas Saußen, helping with the interview with Prof.Dr.rer.nat. Krieg and video of iGEM final presentation 2014

• Technik Garage
• Dr. Stephan Schlote, supplying of DIY water bath

• Others
• Prof. Dr. Frank Eiden, giving an interview at Symposium
• Dr.Julia Frunzke, giving an interview at Symposium
• M.A. Dietrich Hunold, helping us with Alumni
• Jay Keasling, giving an interview at Symposium and project feedback
• Udo Kosfeld, helping us with certain electronic components and how to work with them which helped us develop the final circuit design
• Dipl. -Ing. Martina Mühlmann, for Biolector access in AVT (Institute of Bioprocess Engineering)
• Synbiota Team, for
• M.A. Angela Poth, helping us with Alumni
• Prof. Sheng Yang, providing pCas plasmid
• Wisam Zureik, shooting, producing and cutting of teaser, vision and human practice video

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Contact

CC-BY-SA iGEM Aachen 2015