Team:Hamburg/Parts
Parts
Our ultimate goal was to create a Biobrick enabling light-inducible miRNA2911 production and heat-inducible cell lysis for the release afterwards in one plasmid (see figure). Despite not reaching this goal so far, we completed important intermediate steps successfully heading towards it.
![](https://static.igem.org/mediawiki/2015/b/b6/Hamburg_parts4.jpg)
Two basic parts contain the miRNA and GroEL. Two composite parts contain a constitutive promoter + miRNA system (where the constitutive promoter replaces the light-inducible promoter as an intermediate step) and a GroEL + blue pigment system (where the blue pigment replaces the cell lysis protein PezT for now). Further steps would be to design a light promoter + miRNA biobrick and a GroEL + PezT Biobrick, to then combine them by 3A-Assembly for our ultimate goal.
![Our concept](https://static.igem.org/mediawiki/2015/8/89/Hamburg_mainpic.jpg)
Team Parts
<groupparts>iGEM015 Hamburg</groupparts>