Team:Paris Bettencourt/Notebook/VitaminB12
Ferment It Yourself
iGEM Paris-Bettencourt 2O15
- Background
- Design
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Notebook
Vitamin A Vitamin B2 Vitamin B12 Phytase Riboswitch Differentiation on E. coli Differentiation on S. cerevisiae Manufacturing Idli and Micro-organisms Transformation resultsTransformed product pKV6 pSIP411 p15.01 p15.02 negative control - (ery 150µg/mL):
500 colonies, no background
- (ery 10µg/mL):
no growth - (ery 150µg/mL):
lawn of bacteria
- spreading of 170µL:
41 isolated colonies -> isolation of three transformants: T1, T2 and T3 - spreading of 50µL:
3 colonies on a lawny background
- (ery 10µg/mL):
lawn of bacteria - (ery 150µg/mL):
lawny background
no growth - (ery 150µg/mL):