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Revision as of 16:56, 19 November 2015
Catalytic RNA – Ribozymes
![](https://static.igem.org/mediawiki/2015/4/43/Fig1HHR.png)
The secondary structure of a Hammerhead Ribozyme (HHR). Arrow indicates position of cleavage between third and first stem. First and second stem are stem loops while stem three is open.
In 1982 the first catalytic RNA (Ribozyme): a self-spicing intron from Tetrahymena pre-rRNA was described.
Another ribozyme that is related to the HHR is the hepatitis δ virus ribozyme (HDV).
![](https://static.igem.org/mediawiki/2015/c/cd/Fig2.png)
Naturally occurring Hairpin Ribozyme with cleavage site indicated by arrow.
Both of the mentioned types of ribozymes are found in satellite RNA of plant origin
Our idea of working with functional nucleic acids originated from this system. We were fascinated by the vast variety of processes that they can perform and started digging deeper into the potential of nucleic acids as tools. During this process we came across interesting systems amongst which we found the twin ribozyme (Fig. 3). Another famous hairpin-ribozyme-derived functional nucleic acid developed by Müller.
Next to the directed evolution of existing ribozymes in vitro selection methods
![](https://static.igem.org/mediawiki/2015/3/35/Fig3Twin.png)
Secondary structure of the Twin Ribozyme. With cleavage and ligation sites indicated by arrows. The Hairpin Ribozyme is derived from the Hairpin Ribozyme.