Difference between revisions of "Team:Paris Bettencourt/Notebook/Phytase"
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<br><h1 class="date two">September 4th</h1> | <br><h1 class="date two">September 4th</h1> | ||
− | After the transformation of | + | After the transformation of September 2nd, any culture is observable on plates. The tansformation are not function. We restart transformation, but we transform with Cre and RFP gene. It is more interesting because we not forced to simply remove the resistance gene, we can make a double identification. It helps to identify yeast that is already integrated in place of the resistance gene PHO85 (they cultivate on agar with geneticin) and we also integrate the RFP gene in place of PHO80 gene (they will be red). |
<br><h1 class="date two">September 8th</h1> | <br><h1 class="date two">September 8th</h1> | ||
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Second step, we make PCR purification, and check the result with electrophorese<br> | Second step, we make PCR purification, and check the result with electrophorese<br> | ||
− | <b>Protocol</b> :<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/PCR_purification"> PCR purification</a><br> | + | <b>Protocol</b> :<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/PCR_purification"> PCR purification</a> |
+ | <br> | ||
<div class="column-left"> | <div class="column-left"> | ||
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<b>Protocol</b> :<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat_shock_transformation_for_yeast"> Transformation of yeast</a><br> | <b>Protocol</b> :<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat_shock_transformation_for_yeast"> Transformation of yeast</a><br> | ||
− | Fourth step, we cultived the transforming | + | Fourth step, we cultived the transforming yeasts in YPD agar medium with geneticin overnight. |
<h2> Analyse the result of titration phytic acid of September 2nd</h2> | <h2> Analyse the result of titration phytic acid of September 2nd</h2> | ||
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<p class="legend"><b>Figure 19 :</b>curve of gamme</p></div> | <p class="legend"><b>Figure 19 :</b>curve of gamme</p></div> | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
− | With calculations which are in the protocol, we have the concentration in our samples | + | With calculations which are in the protocol, we have the concentration in our samples.<br> |
<b>Protocol :</b><a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/titration-acid-phytic">Titration of phytc acid</a><br> | <b>Protocol :</b><a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/titration-acid-phytic">Titration of phytc acid</a><br> |
Revision as of 10:16, 18 September 2015