Difference between revisions of "Team:Paris Bettencourt/Notebook/Phytase"
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<div id="notebookContent"> | <div id="notebookContent"> | ||
<a name="august" class="anchor"><h1></h1></a> | <a name="august" class="anchor"><h1></h1></a> | ||
− | <h1 class="date | + | <h1 class="date two">August 8th</h1> |
<h2>Design primers</h2> | <h2>Design primers</h2> | ||
<h3>Gene PHO85</h3> | <h3>Gene PHO85</h3> | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 12nd</h1> |
<h2>Culture</h2> | <h2>Culture</h2> | ||
<p>Inoculate 100µL of <i>Saccharomyces cerevisiae SK1</i> on YPD medium overnight (at 30°C). | <p>Inoculate 100µL of <i>Saccharomyces cerevisiae SK1</i> on YPD medium overnight (at 30°C). | ||
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<div style="clear:both"></div> | <div style="clear:both"></div> | ||
− | <br><h1 class="date | + | <br><h1 class="date two">August 13th</h1> |
<h2>PCR Purification</h2> | <h2>PCR Purification</h2> | ||
Protocol : | Protocol : | ||
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Plated one colony of <i>Saccharomyces cerevisiae SK1</i> in 5mL liquid YPD medium and let's grow overnight.<br> | Plated one colony of <i>Saccharomyces cerevisiae SK1</i> in 5mL liquid YPD medium and let's grow overnight.<br> | ||
− | <br><h1 class="date | + | <br><h1 class="date two">August 14th</h1> |
Transformation of yeast<br> | Transformation of yeast<br> | ||
Protocol: | Protocol: | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 17th</h1> |
<h2>Result of plates:</h2> | <h2>Result of plates:</h2> | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 18th</h1> |
<h2>Verification of the new negative control</h2> | <h2>Verification of the new negative control</h2> | ||
<div class="column-right"><p> | <div class="column-right"><p> | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 19th</h1> |
<h2>Colony PCR</h2> | <h2>Colony PCR</h2> | ||
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<div style="clear:both"></div> | <div style="clear:both"></div> | ||
− | <br><h1 class="date | + | <br><h1 class="date two">August 20th</h1> |
<h2>Result of colony PCR (August 19th)</h2> | <h2>Result of colony PCR (August 19th)</h2> | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 24th</h1> |
<h2>Yeast lysis with NaOH</h2> | <h2>Yeast lysis with NaOH</h2> | ||
<b>Protocol:</b> <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Yeast_lysis_with_NaOH"> Yeast lysis with NaOH</a><br> | <b>Protocol:</b> <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Yeast_lysis_with_NaOH"> Yeast lysis with NaOH</a><br> | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 25th</h1> |
<h2>Result of PCR (August 24th)</h2> | <h2>Result of PCR (August 24th)</h2> | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 26th</h1> |
<h2>Colony PCR</h2> | <h2>Colony PCR</h2> | ||
<p>To make the colony PCR, we need to lysis yeasts' wall. We realized the lysis with NaOH, but it did not work. | <p>To make the colony PCR, we need to lysis yeasts' wall. We realized the lysis with NaOH, but it did not work. | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 27th</h1> |
<h2>Result of PCR (August 26th)</h2> | <h2>Result of PCR (August 26th)</h2> | ||
<div class="column-right"> | <div class="column-right"> | ||
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− | <br><h1 class="date | + | <br><h1 class="date two">August 28th</h1> |
<h2>Phytic acid dosage in different strains</h2> | <h2>Phytic acid dosage in different strains</h2> | ||
<div class="column-left"> | <div class="column-left"> | ||
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The protocole is:<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat_shock_transformation_for_yeast"> transformation of yeast</a> | The protocole is:<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat_shock_transformation_for_yeast"> transformation of yeast</a> | ||
− | <br><h1 class="date | + | <br><h1 class="date two">August 30th</h1> |
<h2>The transformation on yeats with CRE gene</h2> | <h2>The transformation on yeats with CRE gene</h2> | ||
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<div style="clear:both"></div> | <div style="clear:both"></div> | ||
− | <br><h1 class="date | + | <br><h1 class="date three">September 2nd</h1> |
<h2> Transformation</h2> | <h2> Transformation</h2> | ||
We start again the transformation of august 28th, with the same protocol. | We start again the transformation of august 28th, with the same protocol. |
Revision as of 19:14, 3 September 2015