Difference between revisions of "Team:Paris Bettencourt/Protocols"

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<div id="protocolsGallerie">
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  <div class="box">
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    <div class="innerBox yellow">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols#AnalyticalDigestion">
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        <p>Analytical digestion</p>
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        <img src="https://static.igem.org/mediawiki/2015/5/5e/ParisBettencourt_protocolsPipetting.JPG">
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        <div class="textBox" id="AnalitycalDigestion">
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<b>Analytical digestion protocol</b><br>
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<ul>
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  <li>Prepare the following mix:
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  <ul>
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    <li>2µL 10X Digestion buffer
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    <li>0.5µL Eco31I
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    <li>0.5µL BbsI
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    <li>2µL of DNA (200ng)
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    <li>15µL water
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  </ul>
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  <li>incube 1h at 37°C
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</ul>
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      </div>
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols#Annealing">
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        <p>Annealing</p>
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        <img src="https://static.igem.org/mediawiki/2015/1/11/ParisBettencourt_protocolsGel.JPG">
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        <div class="textBox" id="Annealing">
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<b>Annealing Protocol</b>
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<ul>
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<li>Phosphorylation of the oligos
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<ul>
 +
  <li>        5.6μL DNAse/RNAse free water
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  <li> 6.0μL  o15.011 (10µM)
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  <li> 6.0μL  o15.012 (10µM)
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  <li> 2.0μL  10X T4 DNA ligase buffer
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  <li> 0.4μL  T4 PolyNucleotide Kinase
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Total:  20μL
  
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</ul>
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<br/>
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  <li>incube 30min at 37°C
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  <li>add 1μL of 1M NaCl
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  <li>incube 5min at 95°C
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  <li>let the mix cool down
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  <li>use 2μL of the mix as a 10X solution
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</ul>
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        </div>
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox magenta">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Electroporation">
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        <p>Electroporation</p>
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        <img src="https://static.igem.org/mediawiki/2015/2/24/ParisBettencourt_protocolsCentrifuge.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox yellow">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Electrocompetent Cells Preparation">
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        <p>Electrocompetent Cells Preparation</p>
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        <img src="https://static.igem.org/mediawiki/2015/d/da/ParisBettencourt_protocolsErlen.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Digestion">
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        <p>Digestion</p>
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        <img src="https://static.igem.org/mediawiki/2015/6/6a/ParisBettencourt_protocolsTubesOnIce.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox magenta">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Electrocompetent Cells Preparation for Lactobacillus Plantarum">
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        <p>Electrocompetent Cells Preparation for <i>Lactobacillus plantarum</i></p>
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        <img src="https://static.igem.org/mediawiki/2015/e/ec/ParisBettencourt_protocolsFrozenEnzymes.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox yellow">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat Shock transformation">
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        <p>Heat Shock transformation</p>
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        <img src="https://static.igem.org/mediawiki/2015/0/0d/ParisBettencourt_protocolsBanana.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Titration protocols for vitamin A">
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        <p>Titration protocols for vitamin A</p>
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox magenta">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Yeast lysis with NaOH">
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        <p>Yeast lysis with NaOH</p>
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        <img src="https://static.igem.org/mediawiki/2015/c/ce/ParisBettencourt_protocolsTubesIncubator.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox yellow">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat shock transformation for yeast">
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        <p>Heat shock transformation for yeast</p>
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        <img src="https://static.igem.org/mediawiki/2015/5/5e/ParisBettencourt_protocolsPipetting.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Ligation">
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        <p>Ligation</p>
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        <img src="https://static.igem.org/mediawiki/2015/3/34/ParisBettencourt_protocolsPCR.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox magenta">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Titration protocols for vitamin B2 with HPLC">
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        <p>Titration protocols for vitamin B2 with HPLC</p>
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        <img src="https://static.igem.org/mediawiki/2015/1/1c/ParisBettencourt_protocolsTubeUnderHood.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox yellow">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Idli receipe">
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        <p>Idli recipe</p>
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        <img src="https://static.igem.org/mediawiki/2015/5/55/ParisBettencourt_protocolsIdli.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Miniprep protocol using a QIAGEN kit">
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        <p>Miniprep protocol using a QIAGEN kit</p>
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        <img src="https://static.igem.org/mediawiki/2015/e/ec/ParisBettencourt_protocolsQiagen.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/PCR purification">
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        <p>PCR purification</p>
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        <img src="https://static.igem.org/mediawiki/2015/2/23/ParisBettencourt_protocolsPipette.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/PCR">
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        <p>PCR</p>
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        <img src="https://static.igem.org/mediawiki/2015/3/34/ParisBettencourt_protocolsPCR.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/gel-purification">
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        <p>Gel purification</p>
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        <img src="https://static.igem.org/mediawiki/2015/1/11/ParisBettencourt_protocolsGel.JPG">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/yeast-dna-extraction-dneasy-blood-and-tissue-kit">
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        <p>Yeast DNA extraction using DNeasy® Blood & Tissue Kit [QIAGEN]</p>
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        <img src="">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/titration-acid-phytic">
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        <p>Titration of phytic acid using Megazyme© Kit</p>
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        <img src="">
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      </a>
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    </div>
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  </div>
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  <div class="box">
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    <div class="innerBox cyan">
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      <a https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Gel_Electrophoresis">
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        <p>Gel Electrophoresis with SYBR safe</p>
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        <img src="">
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      </a>
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    </div>
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  </div>
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</div>
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Revision as of 08:36, 31 August 2015