Difference between revisions of "Team:Paris Bettencourt/Protocols"

Line 71: Line 71:
 
<li>Phosphorylation of the oligos
 
<li>Phosphorylation of the oligos
 
<ul>
 
<ul>
−
   <li>       5.6μL DNAse/RNAse free water
+
   <li> 5.6μL DNAse/RNAse free water
−
   <li> 6.0μL  o15.011 (10µM)
+
   <li> 6.0μL  oligo 1 (10µM)
−
   <li> 6.0μL  o15.012 (10µM)
+
   <li> 6.0μL  oligo 2 (10µM)
 
   <li> 2.0μL  10X T4 DNA ligase buffer
 
   <li> 2.0μL  10X T4 DNA ligase buffer
 
   <li> 0.4μL  T4 PolyNucleotide Kinase
 
   <li> 0.4μL  T4 PolyNucleotide Kinase

Revision as of 15:44, 1 September 2015