Difference between revisions of "Team:Paris Bettencourt/Notebook/Phytase"
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<h2> Transformation</h2> | <h2> Transformation</h2> | ||
We transform the yeast (...) with the resistance to geneticin and the CRE gene. We delete the PHO 85 genes and we replace it by the resistance gene.<br> | We transform the yeast (...) with the resistance to geneticin and the CRE gene. We delete the PHO 85 genes and we replace it by the resistance gene.<br> | ||
− | The CRE gene is... | + | The CRE gene is the sequence that little be cut thanks (...) and reconbined the gene without the sequence between the CRE gene <br> |
The protocole is:<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat_shock_transformation_for_yeast"> transformation of yeast</a> | The protocole is:<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat_shock_transformation_for_yeast"> transformation of yeast</a> | ||
<br><h1 class="date one">August 30th</h1> | <br><h1 class="date one">August 30th</h1> | ||
<h2>The transformation on yeats with CRE gene</h2> | <h2>The transformation on yeats with CRE gene</h2> | ||
− | We watching Any culture in the plate YPD + geneticin. The yeast is don't resistante to geneticin, the yeast has not introduced the resistance gene. Transformation does not work. | + | |
+ | <div class="column-left"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/3/37/ParisBettencourt_Gel_28.08_Gradient_yeast_transfo_2temp_80-85-FRT.png" width="550px"> | ||
+ | <p class="legend"><b>Figure 16 :</b> Plate YPD + geneticin plated with transforme yeast</p><br> | ||
+ | </div> | ||
+ | <div class="column-right"><p>We watching Any culture in the plate YPD + geneticin. The yeast is don't resistante to geneticin, the yeast has not introduced the resistance gene. Transformation does not work.</p></div> | ||
+ | |||
<br><h1 class="date one">September 2nd</h1> | <br><h1 class="date one">September 2nd</h1> |
Revision as of 09:22, 2 September 2015