Difference between revisions of "Team:Paris Bettencourt/Notebook/Phytase"
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<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<h2> Transformation</h2> | <h2> Transformation</h2> | ||
− | We transform the yeast (...) with the resistance to geneticin and the CRE gene. We delete the PHO 85 genes and we replace it by the resistance gene.< | + | |
+ | <div class="column-right"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/e/e7/PARISBETTENCOURT_CRE.png" width="550px"> | ||
+ | <p class="legend"><b>Figure 16 :</b>Principle of Cre recombinase</p><br> | ||
+ | </div> | ||
+ | <div class="column-left"><p>We transform the yeast (...) with the resistance to geneticin and the CRE gene. We delete the PHO 85 genes and we replace it by the resistance gene.</p></div> | ||
+ | <div style="clear:both"></div> | ||
+ | |||
The CRE gene is the sequence that little be cut thanks Cre recombinase and reconbined the gene without the sequence between the CRE gene <br> | The CRE gene is the sequence that little be cut thanks Cre recombinase and reconbined the gene without the sequence between the CRE gene <br> | ||
The protocole is:<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat_shock_transformation_for_yeast"> transformation of yeast</a> | The protocole is:<a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols/Heat_shock_transformation_for_yeast"> transformation of yeast</a> |
Revision as of 10:00, 2 September 2015