Team:Paris Bettencourt/Protocols

Ferment It Yourself

iGEM Paris-Bettencourt 2O15

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Notebook

Vitamin A	Vitamin B2	Vitamin B12
Phytase	Riboswitch	Differentiation on E. coli
Differentiation on S. cerevisiae	Manufacturing	Idli and Micro-organisms

Analytical digestion
Annealing
Digestion
Electrocompetent Cells Preparation
Electrocompetent Cells Preparation for Lactobacillus Plantarum
Electroporation
Heat Shock transformation
Idli receipe
Ligation
Miniprep protocol using a QIAGEN kit
PCR purification
Titration protocols for vitamin A
Titration protocols for vitamin B2 with HPLC