Difference between revisions of "Team:Paris Bettencourt/Protocols"
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+ | <div class="textBox" id="Electroporation"> | ||
+ | <h3>Electroporation</h3> | ||
+ | <ul> | ||
+ | <li>Thaw electrocompetent cells on ice | ||
+ | <li>Add 2µL of ligation product or 0.5µL of native plasmid to the cells | ||
+ | <li>Transfer the cells in an 0.2mm electroporation cuvette | ||
+ | <li>put the cuvette in the electroporation device and pulse the cells at 2.5kV, 200 Ohms and 25µF | ||
+ | <li>add 200µL of LB right after pulsing | ||
+ | <li>recover 2 hours at 37°C | ||
+ | <li>plate 200µL and 50µL of the cells on LB + erythromycin (200µg/mL), incubate overnight at 37°C | ||
+ | </ul> | ||
+ | </div> | ||
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<div id="protocolsGallerie"> | <div id="protocolsGallerie"> | ||
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<div class="box"> | <div class="box"> | ||
<div class="innerBox magenta"> | <div class="innerBox magenta"> | ||
− | <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols | + | <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols#Electroporation"> |
<div class="ptext"><p>Electroporation</p></div> | <div class="ptext"><p>Electroporation</p></div> | ||
− | <img src=" | + | <img src=""> |
</a> | </a> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2015/5/5e/ParisBettencourt_protocolsPipetting.JPG"> | <img src="https://static.igem.org/mediawiki/2015/5/5e/ParisBettencourt_protocolsPipetting.JPG"> | ||
<img src="https://static.igem.org/mediawiki/2015/1/11/ParisBettencourt_protocolsGel.JPG"> | <img src="https://static.igem.org/mediawiki/2015/1/11/ParisBettencourt_protocolsGel.JPG"> | ||
− | + | <img src="https://static.igem.org/mediawiki/2015/2/24/ParisBettencourt_protocolsCentrifuge.JPG"> | |
</html> | </html> | ||
{{Paris_Bettencourt/footer}} | {{Paris_Bettencourt/footer}} |
Revision as of 14:37, 10 September 2015