Difference between revisions of "Team:Paris Bettencourt/Protocols"
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<li>Make aliquots of the desire volume in microcentrifuge tubes and freeze them at -80°C | <li>Make aliquots of the desire volume in microcentrifuge tubes and freeze them at -80°C | ||
</ul> | </ul> | ||
+ | </div> | ||
+ | |||
+ | <div class="textBox" id="Digestion"> | ||
+ | <h3>Digestion</h3> | ||
+ | <ul> | ||
+ | <li>Prepare the following mix: | ||
+ | <ul> | ||
+ | <li>4μL of Enzyme 1 Fast Digest</li> | ||
+ | <li>4μL of Enzyme 2 Fast Digest</li> | ||
+ | <li>4μL of FastAP</li> | ||
+ | <li>12μL of Fast Digest buffer 10X</li> | ||
+ | <li>1 to 3 μg of DNA</li> | ||
+ | <li>up to 120μL of water</li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li>mix by pipetting up and down</li> | ||
+ | <li>incube 10 to 20 minutes at 37°C</li> | ||
+ | <li>incube 10 minutes at 68°C to inactivate the enzymes</li> | ||
+ | </ul> | ||
</div> | </div> | ||
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<div class="box"> | <div class="box"> | ||
<div class="innerBox cyan"> | <div class="innerBox cyan"> | ||
− | <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols | + | <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols#Digestion"> |
<div class="ptext"><p>Digestion</p></div> | <div class="ptext"><p>Digestion</p></div> | ||
<img src="https://static.igem.org/mediawiki/2015/6/6a/ParisBettencourt_protocolsTubesOnIce.JPG"> | <img src="https://static.igem.org/mediawiki/2015/6/6a/ParisBettencourt_protocolsTubesOnIce.JPG"> |
Revision as of 14:42, 10 September 2015