Difference between revisions of "Team:Cambridge-JIC/Safety"
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<h3>Safety concerns in our Project</h3> | <h3>Safety concerns in our Project</h3> | ||
− | <h4><b>Lighting | + | <h4><b>Lighting</b></h4> |
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Some fluorescent molecules require UV light in order to excite them. UV light can be potentially harmful to the eyes and skin if not used with caution. In the project only near-UV LEDs are used, which have a peak in emission at 395nm and are considered safe, as this lies out of the range of 260-270nm [1], which is most harmful to humans. Regardless, all LEDs in the final design of OpenScope are contained within the plastic casing, so the user would not come into contact with any direct light. </p> | Some fluorescent molecules require UV light in order to excite them. UV light can be potentially harmful to the eyes and skin if not used with caution. In the project only near-UV LEDs are used, which have a peak in emission at 395nm and are considered safe, as this lies out of the range of 260-270nm [1], which is most harmful to humans. Regardless, all LEDs in the final design of OpenScope are contained within the plastic casing, so the user would not come into contact with any direct light. </p> | ||
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− | <h4><b> | + | <h4><b>Electronics</b></h4> |
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− | + | OpenScope is a digital microscope, and as such is based on a number of electronics: Raspberry Pi, Raspberry Pi camera, Arduino, stepper motors, etc. The Arduino and the Raspberry Pi boards have 5V/2A power supplies, or alternmatively can be plugged into 9V batteries. These components are to be handled as typical home electronic devices. Detailed wiring instructions are available on the <a href="//2015.igem.org/Team:Cambridge-JIC/Make_Your_Own" class="blue">Make Your Own</a> page to ensure that any potential users do not misuse the equipment. | |
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− | <h4><b> | + | <h4><b>Biological</b></h4> |
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− | The biological side of our project involves standard, routine transformations with fluorescent proteins. For testing of some of our equipment pre-transformed, GFP-expressing <i>Marchantia polymorpha</i> was provided to us. However, as part of our collaboration with the William and Mary iGEM team, we carried out transformations of <i>E.coli</i>. We did this following the same protocol as them and following standard safety procedures. For more information see our <a href=" | + | The biological side of our project involves standard, routine transformations with fluorescent proteins. For testing of some of our equipment pre-transformed, GFP-expressing <i>Marchantia polymorpha</i> was provided to us. However, as part of our collaboration with the William and Mary iGEM team, we carried out transformations of <i>E.coli</i>. We did this following the same protocol as them and following standard safety procedures. For more information see our <a href="//2015.igem.org/Team:Cambridge-JIC/Collaborations" class="blue">Collaborations Page</a>. |
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<h4><b>Glass Cutting</b></h4> | <h4><b>Glass Cutting</b></h4> | ||
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− | Glass cutting was carried out to adapt the shape of dichroic mirrors to fit within the | + | Glass cutting was carried out to adapt the shape of dichroic mirrors to fit within the epicube that we designed for fluorescence microscopy. Precautions were taken to ensure that small fragments of glass do not come into contact with eyes or skin. Glass cutting was carried out on a large tray, preventing stray glass fragments being left around the lab; safety glasses, two pairs of gloves and a lab coat were worn at all times when cutting glass. Risk assessments were completed for the activity and students were supervised by the first-aider on hand. These can be found below (note: signatures were removed from the forms for privacy reasons). </p> |
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<div class="factcol" style="background-color:#1B3056;color:#1B3056;width:30%;float:right"> | <div class="factcol" style="background-color:#1B3056;color:#1B3056;width:30%;float:right"> | ||
+ | <p>In the final design of the epicube, these mirrors are incorporated into platic drawers, so the user does not have to come in direct contact with any pieces of glass.</p> | ||
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Revision as of 01:27, 16 September 2015