Difference between revisions of "Team:Marburg/Protocols"
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− | html, body | + | html, body, img, sub, sup, var, center, label, caption, header, menu, nav{ |
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body { | body { | ||
− | background:url( | + | background-image:url('https://static.igem.org/mediawiki/2015/5/5c/MR_pic_bg_1.jpg'); |
− | + | } | |
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− | + | } | |
− | + | a:visited { | |
− | + | color: #FF6600; | |
+ | } | ||
+ | a:hover { | ||
+ | text-decoration: none; | ||
} | } | ||
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</script> | </script> | ||
+ | |||
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− | <body style="height:100%;min-width: | + | <body style="height:100%;min-width:100%;margin:0px; padding:0px;"> |
− | <div style="position:fixed;width:100%;height: | + | <div style="position:fixed;width:100%; min-width:100%; height:100%;min-height:100%; background-image: url('https://static.igem.org/mediawiki/2015/6/6f/MR_pic_bg6.jpg');background-repeat: no-repeat; |
− | + | background-attachment: fixed;-webkit-background-size: cover; | |
− | + | -moz-background-size: cover; | |
− | + | -o-background-size: cover; | |
− | + | background-size: cover; | |
− | + | margin:0px; padding:0px;"></div> | |
− | + | <div style="position:absolute;margin-left:-680px;left:50%;width:1360px;min-width:1360px;min-height:100%;background-color:white;padding-bottom:10px;"> | |
− | + | <div style="position:fixed;width:1360px;min-width:1360;height:70px;z-index:20;margin-top:0px;"> | |
− | + | <div style="display:table;min-width:1360px;height:100%;"> | |
− | + | <div style="display:table-row;"> | |
− | + | <div style="display:table-cell;width:188px;background:white;"> | |
− | <li><a href="https://2015.igem.org/Team:Marburg/Projects" class="first"><img src="https://static.igem.org/mediawiki/2015/5/56/MR_pic_Projects.png" style="height:60px;text-align:center;"></a> | + | <a href="https://2015.igem.org/Team:Marburg" style="text-align:center;"> |
− | + | <img src="https://static.igem.org/mediawiki/2015/1/14/MR_pic_Home.png" style="position:relative;height:66px;padding-bottom:11px;padding-left:8px;padding-right:8px;"> | |
− | <li><a href="https://2015.igem.org/Team:Marburg/ | + | </a> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Background">Fact Sheets</a></li> | + | </div> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Minicells">NUTRInity - Provide</a></li> | + | <div style="display:table-cell;background:#E0E0E0;padding-left:20px;"> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li> | + | <nav id="nav"> |
− | + | <ul id="navigation" style="margin-top:9px;"> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Projects" class="first"><img src="https://static.igem.org/mediawiki/2015/5/56/MR_pic_Projects.png" style="height:60px;text-align:center;"></a> | |
− | + | <ul> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Description">Overview</a></li> | |
− | </li> | + | <li><a href="https://2015.igem.org/Team:Marburg/Background">Fact Sheets</a></li> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Practices" class="first"><img src="https://static.igem.org/mediawiki/2015/3/3a/MR_pic_HP.png" style="height:60px;"></a> | + | <li><a href="https://2015.igem.org/Team:Marburg/Minicells">NUTRInity - Provide</a></li> |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Design">Future Application</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li> | |
− | + | </ul> | |
− | </li> | + | </li> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Achievements" class="first"><img src="https://static.igem.org/mediawiki/2015/b/ba/MR_pic_Achievements.png" style="height:60px;"></a> | + | <li><a href="https://2015.igem.org/Team:Marburg/Practices" class="first"><img src="https://static.igem.org/mediawiki/2015/3/3a/MR_pic_HP.png" style="height:60px;"></a> |
− | + | <ul> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/iGeneration">iGeneration</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li> | |
− | + | </ul> | |
− | + | </li> | |
− | </li> | + | <li><a href="https://2015.igem.org/Team:Marburg/Achievements" class="first"><img src="https://static.igem.org/mediawiki/2015/b/ba/MR_pic_Achievements.png" style="height:60px;"></a> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Collaborations" class="first"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Collaborations.png" style="height:60px;"></a> | + | <ul> |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Parts">Parts</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Results">Results</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Medal Fulfillment">Medal Fulfillment</a></li> | |
− | + | </ul> | |
− | </li> | + | </li> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Notebook" class="first"><img src="https://static.igem.org/mediawiki/2015/5/53/MR_pic_Notebook.png" style="height:60px;"></a> | + | <li><a href="https://2015.igem.org/Team:Marburg/Collaborations" class="first"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Collaborations.png" style="height:60px;"></a> |
− | + | <ul> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Meetup">MeetUp</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Gameofcells">Game of Cells</a></li> | |
− | + | </ul> | |
− | + | </li> | |
− | </li> | + | <li><a href="https://2015.igem.org/Team:Marburg/Notebook" class="first"><img src="https://static.igem.org/mediawiki/2015/5/53/MR_pic_Notebook.png" style="height:60px;"></a> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Team" class="first"><img src="https://static.igem.org/mediawiki/2015/1/1d/MR_pic_Team.png" style="height:60px;"></a> | + | <ul> |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Labbook">Lab Book</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols">Protocols</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Bibliography">Bibliography</a></li> | |
− | + | </ul> | |
− | + | </li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Team" class="first"><img src="https://static.igem.org/mediawiki/2015/1/1d/MR_pic_Team.png" style="height:60px;"></a> | |
− | </li> | + | <ul> |
− | <li><a href="https://2015.igem.org/Team:Marburg/Safety" class="first"><img src="https://static.igem.org/mediawiki/2015/6/6a/MR_pic_Safety.png" style="height:60px;"></a> | + | <li><a href="https://2015.igem.org/Team:Marburg/Members">Members</a></li> |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Acknowledgement">Acknowledgement</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Attributions">Attributions</a></li> | |
− | + | <li><a href="https://2015.igem.org/Team:Marburg/Gallery">Gallery</a></li> | |
− | </li> | + | </ul> |
+ | </li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Safety" class="first"><img src="https://static.igem.org/mediawiki/2015/6/6a/MR_pic_Safety.png" style="height:60px;"></a> | ||
+ | <ul> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
− | + | </ul> | |
− | + | </nav> | |
− | + | </div> | |
− | <div id="socialmedia" style="display:table-cell;background:#E0E0E0;text-align:right;padding-right:5px;vertical-align:middle;"> | + | <div id="socialmedia" style="display:table-cell;background:#E0E0E0;text-align:right;padding-right:5px;vertical-align:middle;"> |
− | + | <span id="smbuttons"> | |
− | + | <a href="https://www.facebook.com/igemmarburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Facebook.png" style="width:30px;"></a> | |
− | + | <a href="https://twitter.com/igem_marburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/75/MR_pic_Twitter.png" style="width:30px;"></a> | |
− | + | <a id="maillink" href="#" style="padding:5px;" class="second"><img src="https://static.igem.org/mediawiki/2015/a/a0/MR_pic_Mailus.png" style="width:30px;" alt="submit"></a> | |
− | + | <a href="https://2015.igem.org/Main_Page" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/9/9b/MR_pic_Igem.png" style="width:30px;"></a> | |
− | + | </span> | |
− | </div><!-- cell --> | + | </div><!-- cell --> |
− | </div> | + | </div> |
− | </div> | + | </div> |
− | </div><!-- row --> | + | </div><!-- row --> |
− | <div style="position:fixed;height:100%;width:195px;background:#E0E0E0;top:115px;z-index:20;vertical-align:middle | + | <div style="position:fixed;min-height:100%;height:100%;width:195px;background:#E0E0E0;top:115px;z-index:20;vertical-align:middle;"> |
− | + | <span style="margin:0px; padding:0px;display:inline-block;height:100%;vertical-align:middle;"> | |
− | + | </span> | |
− | </div> | + | <img src="https://static.igem.org/mediawiki/2015/1/1c/MR_pic_Sidebanner_Home.png" style="width:184px;vertical-align:middle;"> |
+ | </div> | ||
+ | <div style="position:fixed;margin-left:640px;height:100%;min-height:100%;min-width:40px; width:40px; top:115px;background:#E0E0E0; left:50%;z-index:2;"></div> | ||
+ | <div> | ||
+ | <div style="position:relative;text-align:justify;z-index:1;background:white;font-size:13pt;line-height:150%;min-width:100%;max-width:100%;padding-top:132px; padding-left:235px; padding-right:80px;padding-bottom:70px;box-sizing: border-box;"> | ||
− | <div style=" | + | <div style="z-index:1;text-align:center;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/7/76/MR_pic_PROTO2_Button.png" style="height:60px;"/></div> |
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− | <p> | + | <p>Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.</p> |
− | + | ||
− | < | + | <ul style="list-style-type:square; style="color: #FF6600;"> |
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol1">Agarose gels</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol2">Competent E.Coli cells (Electroporation)</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol3">Competent E. coli cells (RbCl)</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol27">Congo Red Staining</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol28">Crystal Violet Staining</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol5">CPEC Standard</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol6">Digests</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol7">Electrocompetent E. coli cells</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol8">GeneJET PCR Purification Kit</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol9">Gibson Assembly® Master Mix – Assembly</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol10">Glycerol stock generation</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol29">Ligation</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol11">Macherey-Nagel Plasmid DNA Purification</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol12">Modified Gel Extraction Protocol</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol13">PCR Standard</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol14">PCR only with primer</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol15">Preparation of Mini Cells</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol30">SDS-Page</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol16">Transformation into chemocompetent (RbCl) cells</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol17">Transformation into electrocompetent cells</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol18">Transformation into not competent E.coli cells</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol19">Transformation into yeast</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol20">QIAprep® Spin Miniprep Kit Quick-StartProtocol</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol22">QIAquick® PCR Purification Kit</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol23">Yeast Miniprep</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol24">Zymo DNA Clean & Concentrator™-5</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol25">Zymo Frozen Yeast Tranformation II Kit</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol26">Zyppy™ Plasmid Midiprep Kit</a></li> | ||
+ | </ul> | ||
+ | <div><br><br></div> | ||
+ | </div> | ||
− | < | + | <div style="position:absolute;text-align:center;z-index:2;background:#e0e0e0;line-height:150%;bottom:0px;padding-top:3px;margin-bottom:0px; padding-left:235px; padding-right:80px;min-height:60px;min-width:100%;max-width:100%;box-sizing:border-box;clear:both;"><!----> |
− | + | <div> | |
+ | <span style="margin-right:60px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/1/1d/MR_pic_syn.png" style="height:40px;padding:10px;"/> | ||
+ | </span> | ||
+ | <span> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/5/58/MR_pic_mpii.png" style="height:40px;padding:10px;"/> | ||
+ | </span> | ||
+ | <span style="margin-left:60px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/f/f0/MR_pic_unii.png" style="height:40px;padding:10px;"/> | ||
+ | </span> | ||
+ | </div> | ||
+ | <span style="font-size:8pt; color:white;"> iGEM Marburg - ZSM Karl-von-Frisch-Straße 16, D - 35043 Marburg</span> | ||
+ | </div> | ||
+ | </div> | ||
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Latest revision as of 17:27, 16 September 2015
Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.
- Agarose gels
- Competent E.Coli cells (Electroporation)
- Competent E. coli cells (RbCl)
- Competent yeast for electroporation
- Congo Red Staining
- Crystal Violet Staining
- CPEC Standard
- Digests
- Electrocompetent E. coli cells
- GeneJET PCR Purification Kit
- Gibson Assembly® Master Mix – Assembly
- Glycerol stock generation
- Ligation
- Macherey-Nagel Plasmid DNA Purification
- Modified Gel Extraction Protocol
- PCR Standard
- PCR only with primer
- Preparation of Mini Cells
- SDS-Page
- Transformation into chemocompetent (RbCl) cells
- Transformation into electrocompetent cells
- Transformation into not competent E.coli cells
- Transformation into yeast
- QIAprep® Spin Miniprep Kit Quick-StartProtocol
- QIAquick® PCR Purification Kit
- Yeast Miniprep
- Zymo DNA Clean & Concentrator™-5
- Zymo Frozen Yeast Tranformation II Kit
- Zyppy™ Plasmid Midiprep Kit