Difference between revisions of "Team:Marburg/Protocols"

 
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/* General Style */
 
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html, body, div, img, sub, sup, var, center, ol, label, table, caption, header, menu, nav {
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body {
 
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background-image:url('https://static.igem.org/mediawiki/2015/5/5c/MR_pic_bg_1.jpg');
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/* Navigation */
 
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<div style="position:fixed;width:100%; min-width:100%; height:100%;min-height:100%; background-image: url('https://static.igem.org/mediawiki/2015/6/6f/MR_pic_bg6.jpg');background-repeat: no-repeat;
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<a href="https://2015.igem.org/Team:Marburg" style="text-align:center;">
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<div style="display:table-row;">
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<ul id="navigation" style="margin-top:9px;">
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<div style="display:table-cell;width:188px;background:white;">
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<li><a href="https://2015.igem.org/Team:Marburg/Projects" class="first"><img src="https://static.igem.org/mediawiki/2015/5/56/MR_pic_Projects.png" style="height:60px;text-align:center;"></a>
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<a href="https://2015.igem.org/Team:Marburg" style="text-align:center;">
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<ul>
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<img src="https://static.igem.org/mediawiki/2015/1/14/MR_pic_Home.png" style="position:relative;height:66px;padding-bottom:11px;padding-left:8px;padding-right:8px;">
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<li><a href="https://2015.igem.org/Team:Marburg/Overview">Overview</a></li>  
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</a>
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<li><a href="https://2015.igem.org/Team:Marburg/Background">Fact Sheets</a></li>
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</div>
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<li><a href="https://2015.igem.org/Team:Marburg/Minicells">NUTRInity - Provide</a></li>
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<div style="display:table-cell;background:#E0E0E0;padding-left:20px;">
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<li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li>  
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<nav id="nav">
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<li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li>  
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<ul id="navigation" style="margin-top:9px;">
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<li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Projects" class="first"><img src="https://static.igem.org/mediawiki/2015/5/56/MR_pic_Projects.png" style="height:60px;text-align:center;"></a>
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<li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li>
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<ul>
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</ul>
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<li><a href="https://2015.igem.org/Team:Marburg/Description">Overview</a></li>
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</li>
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                                                                <li><a href="https://2015.igem.org/Team:Marburg/Background">Fact Sheets</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Practices" class="first"><img src="https://static.igem.org/mediawiki/2015/3/3a/MR_pic_HP.png" style="height:60px;"></a>
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<li><a href="https://2015.igem.org/Team:Marburg/Minicells">NUTRInity - Provide</a></li>
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<ul>
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<li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li>  
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<li><a href="https://2015.igem.org/Team:Marburg/iGeneration">iGeneration</a></li>  
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<li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li>  
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<li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Design">Future Application</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li>  
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<li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li>
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        <li><a href="https://2015.igem.org/Team:Marburg/FutureApplication">Future Application</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li>
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</ul>
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</ul>
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</li>
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</li>
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<li><a href="https://2015.igem.org/Team:Marburg/Achievements" class="first"><img src="https://static.igem.org/mediawiki/2015/b/ba/MR_pic_Achievements.png" style="height:60px;"></a>
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<li><a href="https://2015.igem.org/Team:Marburg/Practices" class="first"><img src="https://static.igem.org/mediawiki/2015/3/3a/MR_pic_HP.png" style="height:60px;"></a>
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<ul>
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<ul>
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<li><a href="https://2015.igem.org/Team:Marburg/Parts">Parts</a></li>  
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<li><a href="https://2015.igem.org/Team:Marburg/iGeneration">iGeneration</a></li>  
−
        <li><a href="https://2015.igem.org/Team:Marburg/Description">Description</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Results">Results</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Medal Fulfillment">Medal Fulfillment</a></li>  
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</ul>
−
</ul>
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</li>
−
</li>
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<li><a href="https://2015.igem.org/Team:Marburg/Achievements" class="first"><img src="https://static.igem.org/mediawiki/2015/b/ba/MR_pic_Achievements.png" style="height:60px;"></a>
−
<li><a href="https://2015.igem.org/Team:Marburg/Collaborations" class="first"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Collaborations.png" style="height:60px;"></a>
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<ul>
−
<ul>
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<li><a href="https://2015.igem.org/Team:Marburg/Parts">Parts</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Meetup">MeetUp</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Results">Results</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Gameofcells">Game of Cells</a></li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Medal Fulfillment">Medal Fulfillment</a></li>  
−
</ul>
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</ul>
−
</li>
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</li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Notebook" class="first"><img src="https://static.igem.org/mediawiki/2015/5/53/MR_pic_Notebook.png" style="height:60px;"></a>
+
<li><a href="https://2015.igem.org/Team:Marburg/Collaborations" class="first"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Collaborations.png" style="height:60px;"></a>
−
<ul>
+
<ul>
−
<li><a href="https://2015.igem.org/Team:Marburg/Labbook">Lab Book</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Meetup">MeetUp</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Protocols">Protocols</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Gameofcells">Game of Cells</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Bibliography">Bibliography</a></li>  
+
</ul>
−
</ul>
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</li>
−
</li>
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<li><a href="https://2015.igem.org/Team:Marburg/Notebook" class="first"><img src="https://static.igem.org/mediawiki/2015/5/53/MR_pic_Notebook.png" style="height:60px;"></a>
−
<li><a href="https://2015.igem.org/Team:Marburg/Team" class="first"><img src="https://static.igem.org/mediawiki/2015/1/1d/MR_pic_Team.png" style="height:60px;"></a>
+
<ul>
−
<ul>
+
<li><a href="https://2015.igem.org/Team:Marburg/Labbook">Lab Book</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Members">Members</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols">Protocols</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Acknowledgement">Acknowledgement</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Bibliography">Bibliography</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Attributions">Attributions</a></li>  
+
</ul>
−
<li><a href="https://2015.igem.org/Team:Marburg/Gallery">Gallery</a></li>  
+
</li>
−
</ul>
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<li><a href="https://2015.igem.org/Team:Marburg/Team" class="first"><img src="https://static.igem.org/mediawiki/2015/1/1d/MR_pic_Team.png" style="height:60px;"></a>
−
</li>
+
<ul>
−
<li><a href="https://2015.igem.org/Team:Marburg/Safety" class="first"><img src="https://static.igem.org/mediawiki/2015/6/6a/MR_pic_Safety.png" style="height:60px;"></a>
+
<li><a href="https://2015.igem.org/Team:Marburg/Members">Members</a></li>  
−
<ul>
+
<li><a href="https://2015.igem.org/Team:Marburg/Acknowledgement">Acknowledgement</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Attributions">Attributions</a></li>  
−
</ul>
+
<li><a href="https://2015.igem.org/Team:Marburg/Gallery">Gallery</a></li>  
−
</li>
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</ul>
 +
</li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Safety" class="first"><img src="https://static.igem.org/mediawiki/2015/6/6a/MR_pic_Safety.png" style="height:60px;"></a>
 +
<ul>
 +
                        <li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li>  
 +
</ul>
 +
</li>
  
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<a href="https://www.facebook.com/igemmarburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Facebook.png" style="width:30px;"></a>
−
<a href="https://twitter.com/igem_marburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/75/MR_pic_Twitter.png" style="width:30px;"></a>
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<a href="https://twitter.com/igem_marburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/75/MR_pic_Twitter.png" style="width:30px;"></a>
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<a id="maillink" href="#" style="padding:5px;" class="second"><img src="https://static.igem.org/mediawiki/2015/a/a0/MR_pic_Mailus.png" style="width:30px;" alt="submit"></a>
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<a id="maillink" href="#" style="padding:5px;" class="second"><img src="https://static.igem.org/mediawiki/2015/a/a0/MR_pic_Mailus.png" style="width:30px;" alt="submit"></a>
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<img src="https://static.igem.org/mediawiki/2015/1/1c/MR_pic_Sidebanner_Home.png" style="width:184px;vertical-align:middle;">
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<div style="position:fixed;margin-left:640px;height:100%;min-height:100%;min-width:40px; width:40px; top:115px;background:#E0E0E0; left:50%;z-index:2;"></div>
  
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<div>
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<div style="position:relative;text-align:justify;z-index:1;background:white;font-size:13pt;line-height:150%;min-width:100%;max-width:100%;padding-top:132px; padding-left:235px; padding-right:80px;padding-bottom:70px;box-sizing: border-box;">
  
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<div style="position:absolute;text-align:justify;z-index:1;background:none;scroll:scroll-y;left:280px;top:0;margin-top:132px;font-size:13pt;line-height:150%;margin-right:100px;max-width:950px;">
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<div style="z-index:1;text-align:center;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/7/76/MR_pic_PROTO2_Button.png" style="height:60px;"/></div>
  
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<p>Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.</p>
  
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<div style="left:10px;z-index:1;text-align:left;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/7/70/MR_pic_Button_Protocols1.png" style="height:70px;"/></div>
 
  
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<h1>TEXT</h1>
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<ul style="list-style-type:square; style="color: #FF6600;">
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<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol1">Agarose gels</a></li>  
−
<p>
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<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol2">Competent E.Coli cells (Electroporation)</a></li>
−
<ul style="list-style-image: url(https://static.igem.org/mediawiki/2015/1/10/MR_pic_lisstyle.png)">
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol3">Competent E. coli cells (RbCl)</a></li>
−
<li>jmlmnklm</li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li>
−
<li>jmlmnklm</li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol27">Congo Red Staining</a></li>
−
<li>jmlmnklm</li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol28">Crystal Violet Staining</a></li>
−
<li>jmlmnklm</li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol5">CPEC Standard</a></li>
−
<li>jmlmnklm</li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol6">Digests</a></li>
−
<li>jmlmnklm</li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol7">Electrocompetent E. coli cells</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol8">GeneJET PCR Purification Kit</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol9">Gibson Assembly® Master Mix – Assembly</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol10">Glycerol stock generation</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol29">Ligation</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol11">Macherey-Nagel Plasmid DNA Purification</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol12">Modified Gel Extraction Protocol</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol13">PCR Standard</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol14">PCR only with primer</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol15">Preparation of Mini Cells</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol30">SDS-Page</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol16">Transformation into chemocompetent (RbCl) cells</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol17">Transformation into electrocompetent cells</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol18">Transformation into not competent E.coli cells</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol19">Transformation into yeast</a></li>  
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol20">QIAprep® Spin Miniprep Kit Quick-StartProtocol</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol22">QIAquick® PCR Purification Kit</a></li>  
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol23">Yeast Miniprep</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol24">Zymo DNA Clean & Concentrator™-5</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol25">Zymo Frozen Yeast Tranformation II Kit</a></li>  
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol26">Zyppy™ Plasmid Midiprep Kit</a></li>  
 
</ul>
 
</ul>
  
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</p>
 
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</div>
 
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</body>
 
  
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<div style="position:fixed;height:100%;width:40px;background:#E0E0E0;top:105px;right:0px;z-index:0;vertical-align:middle;white-space:nowrap;">
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<div><br><br></div>
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</div>
  
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<img src="https://static.igem.org/mediawiki/2015/f/f0/MR_pic_unii.png" style="height:40px;padding:10px;"/>
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    <span style="font-size:8pt; color:white;"> iGEM Marburg - ZSM Karl-von-Frisch-Straße 16, D - 35043 Marburg</span>
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Latest revision as of 17:27, 16 September 2015

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iGEM Marburg - ZSM Karl-von-Frisch-Straße 16, D - 35043 Marburg