Difference between revisions of "Team:Marburg/Protocols"

 
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html, #bodyContent, body, #container {
 
html, #bodyContent, body, #container {
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/* General Style */
 
/* General Style */
  
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html, body, div, img, sub, sup, var, center, ol, label, table, caption, header, menu, nav {
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body {
 
body {
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background:url(pattern.png) repeat scroll 0 0;
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background-image:url('https://static.igem.org/mediawiki/2015/5/5c/MR_pic_bg_1.jpg');
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}
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}
 
 
 
/* Navigation */
 
/* Navigation */
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ol, {
 
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#nav {
 
#nav {
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}
 
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a {
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</style>
 
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});
 
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<!--HTML-MENU-->
 
<!--HTML-MENU-->
  
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<body style="height:100%;min-width:1280px;">
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<body style="height:100%;min-width:100%;margin:0px; padding:0px;">
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<div style="position:fixed;width:100%;height:70px;min-width:1280px;z-index:20;margin-top:0px;">
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<div style="position:fixed;width:100%; min-width:100%; height:100%;min-height:100%; background-image: url('https://static.igem.org/mediawiki/2015/6/6f/MR_pic_bg6.jpg');background-repeat: no-repeat;
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<div style="display:table;width:100%;height:100%;">
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<div style="display:table-row;">
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  -moz-background-size: cover;
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<div style="display:table-cell;width:188px;background:white;">
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  -o-background-size: cover;
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<a href="https://2015.igem.org/Team:Marburg" style="text-align:center;">
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  background-size: cover;
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<img src="https://static.igem.org/mediawiki/2015/1/14/MR_pic_Home.png" style="height:66px;margin:5px 8px;">
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</a>
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<div style="position:absolute;margin-left:-680px;left:50%;width:1360px;min-width:1360px;min-height:100%;background-color:white;padding-bottom:10px;">
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</div>
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<div style="position:fixed;width:1360px;min-width:1360;height:70px;z-index:20;margin-top:0px;">
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<div style="display:table-cell;background:#E0E0E0;padding-left:20px;">
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<div style="display:table;min-width:1360px;height:100%;">
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<nav id="nav">
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<div style="display:table-row;">
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<ul id="navigation" style="margin-top:9px;">
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<div style="display:table-cell;width:188px;background:white;">
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<li><a href="https://2015.igem.org/Team:Marburg/Projects" class="first"><img src="https://static.igem.org/mediawiki/2015/5/56/MR_pic_Projects.png" style="height:60px;text-align:center;"></a>
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<a href="https://2015.igem.org/Team:Marburg" style="text-align:center;">
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<ul>
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<img src="https://static.igem.org/mediawiki/2015/1/14/MR_pic_Home.png" style="position:relative;height:66px;padding-bottom:11px;padding-left:8px;padding-right:8px;">
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<li><a href="https://2015.igem.org/Team:Marburg/Overview">Overview</a></li>  
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</a>
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<li><a href="https://2015.igem.org/Team:Marburg/Background">Fact Sheets</a></li>
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</div>
−
<li><a href="https://2015.igem.org/Team:Marburg/Minicells">NUTRInity - Provide</a></li>
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<div style="display:table-cell;background:#E0E0E0;padding-left:20px;">
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<li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li>  
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<nav id="nav">
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<li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li>  
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<ul id="navigation" style="margin-top:9px;">
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<li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Projects" class="first"><img src="https://static.igem.org/mediawiki/2015/5/56/MR_pic_Projects.png" style="height:60px;text-align:center;"></a>
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<li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li>
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<ul>
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</ul>
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<li><a href="https://2015.igem.org/Team:Marburg/Description">Overview</a></li>
−
</li>
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                                                                <li><a href="https://2015.igem.org/Team:Marburg/Background">Fact Sheets</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Practices" class="first"><img src="https://static.igem.org/mediawiki/2015/3/3a/MR_pic_HP.png" style="height:60px;"></a>
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<li><a href="https://2015.igem.org/Team:Marburg/Minicells">NUTRInity - Provide</a></li>
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<ul>
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<li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/iGeneration">iGeneration</a></li>  
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<li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Design">Future Application</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li>  
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<li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li>
−
        <li><a href="https://2015.igem.org/Team:Marburg/FutureApplication">Future Application</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li>
−
</ul>
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</ul>
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</li>
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</li>
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<li><a href="https://2015.igem.org/Team:Marburg/Achievements" class="first"><img src="https://static.igem.org/mediawiki/2015/b/ba/MR_pic_Achievements.png" style="height:60px;"></a>
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<li><a href="https://2015.igem.org/Team:Marburg/Practices" class="first"><img src="https://static.igem.org/mediawiki/2015/3/3a/MR_pic_HP.png" style="height:60px;"></a>
−
<ul>
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<ul>
−
<li><a href="https://2015.igem.org/Team:Marburg/Parts">Parts</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/iGeneration">iGeneration</a></li>  
−
        <li><a href="https://2015.igem.org/Team:Marburg/Description">Description</a></li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Results">Results</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Medal Fulfillment">Medal Fulfillment</a></li>  
+
</ul>
−
</ul>
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</li>
−
</li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Achievements" class="first"><img src="https://static.igem.org/mediawiki/2015/b/ba/MR_pic_Achievements.png" style="height:60px;"></a>
−
<li><a href="https://2015.igem.org/Team:Marburg/Collaborations" class="first"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Collaborations.png" style="height:60px;"></a>
+
<ul>
−
<ul>
+
<li><a href="https://2015.igem.org/Team:Marburg/Parts">Parts</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Meetup">MeetUp</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Results">Results</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Gameofcells">Game of Cells</a></li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Medal Fulfillment">Medal Fulfillment</a></li>  
−
</ul>
+
</ul>
−
</li>
+
</li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Notebook" class="first"><img src="https://static.igem.org/mediawiki/2015/5/53/MR_pic_Notebook.png" style="height:60px;"></a>
+
<li><a href="https://2015.igem.org/Team:Marburg/Collaborations" class="first"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Collaborations.png" style="height:60px;"></a>
−
<ul>
+
<ul>
−
<li><a href="https://2015.igem.org/Team:Marburg/Labbook">Lab Book</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Meetup">MeetUp</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Protocols">Protocols</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Gameofcells">Game of Cells</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Bibliography">Bibliography</a></li>  
+
</ul>
−
</ul>
+
</li>
−
</li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Notebook" class="first"><img src="https://static.igem.org/mediawiki/2015/5/53/MR_pic_Notebook.png" style="height:60px;"></a>
−
<li><a href="https://2015.igem.org/Team:Marburg/Team" class="first"><img src="https://static.igem.org/mediawiki/2015/1/1d/MR_pic_Team.png" style="height:60px;"></a>
+
<ul>
−
<ul>
+
<li><a href="https://2015.igem.org/Team:Marburg/Labbook">Lab Book</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Members">Members</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols">Protocols</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Acknowledgement">Acknowledgement</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Bibliography">Bibliography</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Attributions">Attributions</a></li>  
+
</ul>
−
<li><a href="https://2015.igem.org/Team:Marburg/Gallery">Gallery</a></li>  
+
</li>
−
</ul>
+
<li><a href="https://2015.igem.org/Team:Marburg/Team" class="first"><img src="https://static.igem.org/mediawiki/2015/1/1d/MR_pic_Team.png" style="height:60px;"></a>
−
</li>
+
<ul>
−
<li><a href="https://2015.igem.org/Team:Marburg/Safety" class="first"><img src="https://static.igem.org/mediawiki/2015/6/6a/MR_pic_Safety.png" style="height:60px;"></a>
+
<li><a href="https://2015.igem.org/Team:Marburg/Members">Members</a></li>  
−
<ul>
+
<li><a href="https://2015.igem.org/Team:Marburg/Acknowledgement">Acknowledgement</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Attributions">Attributions</a></li>  
−
</ul>
+
<li><a href="https://2015.igem.org/Team:Marburg/Gallery">Gallery</a></li>  
−
</li>
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</ul>
 +
</li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Safety" class="first"><img src="https://static.igem.org/mediawiki/2015/6/6a/MR_pic_Safety.png" style="height:60px;"></a>
 +
<ul>
 +
                        <li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li>  
 +
</ul>
 +
</li>
  
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<a href="https://www.facebook.com/igemmarburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Facebook.png" style="width:30px;"></a>
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<a href="https://www.facebook.com/igemmarburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/71/MR_pic_Facebook.png" style="width:30px;"></a>
−
<a href="https://twitter.com/igem_marburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/75/MR_pic_Twitter.png" style="width:30px;"></a>
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<a href="https://twitter.com/igem_marburg" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/7/75/MR_pic_Twitter.png" style="width:30px;"></a>
−
<a id="maillink" href="#" style="padding:5px;" class="second"><img src="https://static.igem.org/mediawiki/2015/a/a0/MR_pic_Mailus.png" style="width:30px;" alt="submit"></a>
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<a id="maillink" href="#" style="padding:5px;" class="second"><img src="https://static.igem.org/mediawiki/2015/a/a0/MR_pic_Mailus.png" style="width:30px;" alt="submit"></a>
−
<a href="https://2015.igem.org/Main_Page" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/9/9b/MR_pic_Igem.png" style="width:30px;"></a>
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<a href="https://2015.igem.org/Main_Page" class="second" style="padding:5px;"><img src="https://static.igem.org/mediawiki/2015/9/9b/MR_pic_Igem.png" style="width:30px;"></a>
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</div><!-- row -->
 
 
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<div style="position:fixed;height:100%;width:195px;background:#E0E0E0;top:115px;z-index:20;vertical-align:middle;white-space:nowrap;">
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<div style="position:fixed;min-height:100%;height:100%;width:195px;background:#E0E0E0;top:115px;z-index:20;vertical-align:middle;">
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<span style="display:inline-block;height:100%;vertical-align:middle;"></span>
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<span style="margin:0px; padding:0px;display:inline-block;height:100%;vertical-align:middle;">
−
<img src="https://static.igem.org/mediawiki/2015/b/bb/MR_pic_Sidebanner_Notebook.png" style="width:184px;vertical-align:middle;">
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</span>
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</div>
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<img src="https://static.igem.org/mediawiki/2015/1/1c/MR_pic_Sidebanner_Home.png" style="width:184px;vertical-align:middle;">
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</div>
−
 
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<div style="position:fixed;margin-left:640px;height:100%;min-height:100%;min-width:40px; width:40px; top:115px;background:#E0E0E0; left:50%;z-index:2;"></div>
−
<div style="position:absolute;text-align:justify;z-index:1;background:none;scroll:scroll-y;left:280px;top:0;margin-top:132px;font-size:13pt;line-height:150%;margin-right:100px;max-width:950px;">
+
  
 +
<div>
 +
<div style="position:relative;text-align:justify;z-index:1;background:white;font-size:13pt;line-height:150%;min-width:100%;max-width:100%;padding-top:132px; padding-left:235px; padding-right:80px;padding-bottom:70px;box-sizing: border-box;">
  
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<div style="left:10px;z-index:1;text-align:left;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/7/70/MR_pic_Button_Protocols1.png" style="height:70px;"/></div>
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<div style="z-index:1;text-align:center;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/7/76/MR_pic_PROTO2_Button.png" style="height:60px;"/></div>
  
 
<p>Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.</p>
 
<p>Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.</p>
  
  
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<ul style="list-style-type:square;>
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<ul style="list-style-type:square; style="color: #FF6600;">
−
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol1" style="color: FF6600;">Agarose gels</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol1">Agarose gels</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol2">Competent E.Coli cells (Electroporation)</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol2">Competent E.Coli cells (Electroporation)</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol3">Competent E. coli cells (RbCl)</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol3">Competent E. coli cells (RbCl)</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol27">Congo Red Staining</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol28">Crystal Violet Staining</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol5">CPEC Standard</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol5">CPEC Standard</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol6">Digests</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol6">Digests</a></li>
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<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol9">Gibson Assembly® Master Mix – Assembly</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol9">Gibson Assembly® Master Mix – Assembly</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol10">Glycerol stock generation</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol10">Glycerol stock generation</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol29">Ligation</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol11">Macherey-Nagel Plasmid DNA Purification</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol11">Macherey-Nagel Plasmid DNA Purification</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol12">Modified Gel Extraction Protocol</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol12">Modified Gel Extraction Protocol</a></li>  
−
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol13">PCR Standard</a></li>
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol13">PCR Standard</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol14">PCR only with primer</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol14">PCR only with primer</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol15">Preparation of Mini Cells</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol15">Preparation of Mini Cells</a></li>
 +
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol30">SDS-Page</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol16">Transformation into chemocompetent (RbCl) cells</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol16">Transformation into chemocompetent (RbCl) cells</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol17">Transformation into electrocompetent cells</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol17">Transformation into electrocompetent cells</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol18">Transformation into incompetent E.coli cells</a></li>  
+
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol18">Transformation into not competent E.coli cells</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol19">Transformation into yeast</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol19">Transformation into yeast</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol20">QIAprep® Spin Miniprep Kit Quick-StartProtocol</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol20">QIAprep® Spin Miniprep Kit Quick-StartProtocol</a></li>
−
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol21">Measurement Study</a></li>
 
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol22">QIAquick® PCR Purification Kit</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol22">QIAquick® PCR Purification Kit</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol23">Yeast Miniprep</a></li>
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol23">Yeast Miniprep</a></li>
Line 352: Line 363:
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol26">Zyppy™ Plasmid Midiprep Kit</a></li>  
 
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol26">Zyppy™ Plasmid Midiprep Kit</a></li>  
 
</ul>
 
</ul>
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<img src="https://static.igem.org/mediawiki/2015/5/58/MR_pic_mpii.png" style="height:40px;padding:10px;"/>
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<span style="margin-left:60px;">
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<img src="https://static.igem.org/mediawiki/2015/f/f0/MR_pic_unii.png" style="height:40px;padding:10px;"/>
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    <span style="font-size:8pt; color:white;"> iGEM Marburg - ZSM Karl-von-Frisch-Straße 16, D - 35043 Marburg</span>
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Latest revision as of 17:27, 16 September 2015

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iGEM Marburg - ZSM Karl-von-Frisch-Straße 16, D - 35043 Marburg