Difference between revisions of "Team:Marburg/Protocols"
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<li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Marburg/Design">Future Application</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li> | ||
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<li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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<div style="position:relative;text-align:justify;z-index:1;background:white;font-size:13pt;line-height:150%;min-width:100%;max-width:100%;padding-top:132px; padding-left:235px; padding-right:80px;padding-bottom:70px;box-sizing: border-box;"> | <div style="position:relative;text-align:justify;z-index:1;background:white;font-size:13pt;line-height:150%;min-width:100%;max-width:100%;padding-top:132px; padding-left:235px; padding-right:80px;padding-bottom:70px;box-sizing: border-box;"> | ||
| − | <div style="z-index:1;text-align:center;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/7/ | + | <div style="z-index:1;text-align:center;padding-bottom:30px;"><img src="https://static.igem.org/mediawiki/2015/7/76/MR_pic_PROTO2_Button.png" style="height:60px;"/></div> |
<p>Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.</p> | <p>Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.</p> | ||
| − | <ul style="list-style-type:square; style="color: FF6600;"> | + | <ul style="list-style-type:square; style="color: #FF6600;"> |
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol1">Agarose gels</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol1">Agarose gels</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol2">Competent E.Coli cells (Electroporation)</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol2">Competent E.Coli cells (Electroporation)</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol3">Competent E. coli cells (RbCl)</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol3">Competent E. coli cells (RbCl)</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li> | + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol27">Congo Red Staining</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol28">Crystal Violet Staining</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol5">CPEC Standard</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol5">CPEC Standard</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol6">Digests</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol6">Digests</a></li> | ||
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<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol9">Gibson Assembly® Master Mix – Assembly</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol9">Gibson Assembly® Master Mix – Assembly</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol10">Glycerol stock generation</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol10">Glycerol stock generation</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol29">Ligation</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol11">Macherey-Nagel Plasmid DNA Purification</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol11">Macherey-Nagel Plasmid DNA Purification</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol12">Modified Gel Extraction Protocol</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol12">Modified Gel Extraction Protocol</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol13">PCR Standard</a></li> | + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol13">PCR Standard</a></li> |
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol14">PCR only with primer</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol14">PCR only with primer</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol15">Preparation of Mini Cells</a></li> | + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol15">Preparation of Mini Cells</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol30">SDS-Page</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol16">Transformation into chemocompetent (RbCl) cells</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol16">Transformation into chemocompetent (RbCl) cells</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol17">Transformation into electrocompetent cells</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol17">Transformation into electrocompetent cells</a></li> | ||
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</ul> | </ul> | ||
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| + | <div><br><br></div> | ||
</div> | </div> | ||
Latest revision as of 17:27, 16 September 2015
Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.
- Agarose gels
- Competent E.Coli cells (Electroporation)
- Competent E. coli cells (RbCl)
- Competent yeast for electroporation
- Congo Red Staining
- Crystal Violet Staining
- CPEC Standard
- Digests
- Electrocompetent E. coli cells
- GeneJET PCR Purification Kit
- Gibson Assembly® Master Mix – Assembly
- Glycerol stock generation
- Ligation
- Macherey-Nagel Plasmid DNA Purification
- Modified Gel Extraction Protocol
- PCR Standard
- PCR only with primer
- Preparation of Mini Cells
- SDS-Page
- Transformation into chemocompetent (RbCl) cells
- Transformation into electrocompetent cells
- Transformation into not competent E.coli cells
- Transformation into yeast
- QIAprep® Spin Miniprep Kit Quick-StartProtocol
- QIAquick® PCR Purification Kit
- Yeast Miniprep
- Zymo DNA Clean & Concentrator™-5
- Zymo Frozen Yeast Tranformation II Kit
- Zyppy™ Plasmid Midiprep Kit
