Difference between revisions of "Team:Cambridge-JIC/Make Your Own"
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<h1 style="line-height:1.295em"> Make Your Own OpenScope</h1> | <h1 style="line-height:1.295em"> Make Your Own OpenScope</h1> | ||
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| + | <source src="//2015.igem.org/wiki/images/f/fa/CamJIC-Video-Setup.mp4" type="video/mp4"> | ||
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| + | <p>Having problems seeing the video above? Download the video <a href="//2015.igem.org/wiki/images/f/fa/CamJIC-Video-Setup.mp4" class="blue">here</a>.</p> | ||
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Revision as of 18:28, 18 September 2015
If you want to make your very own OpenScope, this is the page for you!
OpenScope is a modular microscope: you can use it in manual mode, or you can add motors for finer control. It is very easy to swap between manual and motorized modes. It is also possible to make the microscope fully battery-powered (ideal for fieldwork!).
You can use your OpenScope for bright-field imaging only, or extend it to include fluorescence imaging or dark-field imaging. We have successfully imaged samples in bright-field, dark-field and GFP fluorescence modes using the materials detailed below. If you want to image RFP or other fluorescent proteins, just make an epicube as explained in the instructions, but change the LEDs, filters and mirrors to suit the FP to be imaged. And if you manage to get it working, we'd love to hear about it!
OpenScope is a modular microscope: you can use it in manual mode, or you can add motors for finer control. It is very easy to swap between manual and motorized modes. It is also possible to make the microscope fully battery-powered (ideal for fieldwork!).
You can use your OpenScope for bright-field imaging only, or extend it to include fluorescence imaging or dark-field imaging. We have successfully imaged samples in bright-field, dark-field and GFP fluorescence modes using the materials detailed below. If you want to image RFP or other fluorescent proteins, just make an epicube as explained in the instructions, but change the LEDs, filters and mirrors to suit the FP to be imaged. And if you manage to get it working, we'd love to hear about it!
Open Scope Documentation by Simon Swan, Katerina Naydenova, Richard Bowman is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
