Difference between revisions of "Team:Freiburg/Protocols"
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<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Competent_Cells">Competent Cells</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Competent_Cells">Competent Cells</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cryo_Stocks">Cryo Stocks</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cryo_Stocks">Cryo Stocks</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/IDT_Procedure">gBlock Procedure</a></li> |
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/PCR_Digest">PCR Template Digest</a></li> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 48: | Line 42: | ||
<h1>Protein Purification & Analysis</h1> | <h1>Protein Purification & Analysis</h1> | ||
<ul class="horizontal_list"> | <ul class="horizontal_list"> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/AmidoBlack">AmidoBlack</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Bead_Regeneration">Ni-NTA Bead Regeneration</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Bead_Regeneration">Ni-NTA Bead Regeneration</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Buffers">Buffers for Analysis</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Buffers">Buffers for Analysis</a></li> | ||
| Line 53: | Line 48: | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Desalting">Desalting of Proetin Solutions (Columns)</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Desalting">Desalting of Proetin Solutions (Columns)</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Dot_Blot">DotBlot</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Dot_Blot">DotBlot</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Freeze_Thaw"> | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Freeze_Thaw">Freezing and Thawing Cells</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Microwave_Destaining">Microwave Destaining</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Overexpression">Overexpression</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Periplasmatic_Preparation">Periplasmic Preparation</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Purification">Purification</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/SDS-PAGE">SDS-Page</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Serum_Preparation">Blood Serum Preparation</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Transfection">Transfection</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Western_Blot">Western Blot</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 65: | Line 63: | ||
<h1>Cell-free Expression</h1> | <h1>Cell-free Expression</h1> | ||
<ul class="horizontal_list"> | <ul class="horizontal_list"> | ||
| − | |||
| − | |||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex_PDMS">Cell-free Expression on PDMS Flow Cell</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Cellfex_PDMS">Cell-free Expression on PDMS Flow Cell</a></li> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
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</ul> | </ul> | ||
</div> | </div> | ||
| Line 82: | Line 70: | ||
<h1>Surface Chemistry</h1> | <h1>Surface Chemistry</h1> | ||
<ul class="horizontal_list"> | <ul class="horizontal_list"> | ||
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Plasmaactivation">Plasma Activation</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/GOPTS_Surface">GOPTS Surface</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/GOPTS_Surface">GOPTS Surface</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/APTES_PDITC">APTES-PDITC Surface</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Ni_NTA_2">Ni-NTA Surface</a></li> | ||
<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Halo_ABCR">HALO Surface (Silane)</a></li> | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Halo_ABCR">HALO Surface (Silane)</a></li> | ||
| − | <li><a href=https://2015.igem.org/Team:Freiburg/Protocols/Halo_Piehler>HALO Surface (Jakob Piehler)</a></li> | + | <!--<li><a href=https://2015.igem.org/Team:Freiburg/Protocols/Halo_Piehler>HALO Surface (Jakob Piehler)</a></li>--> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <!--<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Halo_Wiesmueller">HALO Surface (Wiesmüller)</a></li>--> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Glassslide">Glass Slide Preparation (full)</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/SlidePrep">iRIF Slide Preparation (full)</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Spotting">Spotting</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <!--<li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Strep_Cy5">Regenration of Tris-NTA Slides</a></li>--> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Moulding_Flowcells">Moulding Flowcells</a></li> |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/PDMS_PDITC">PDITC Surface on PDMS</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 99: | Line 89: | ||
<h1>iRIf</h1> | <h1>iRIf</h1> | ||
<ul class="horizontal_list"> | <ul class="horizontal_list"> | ||
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/iRIf_Measurement">iRIf Measurement</a></li> | |
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Protein_Stamping">Protein Stamping</a></li> | |
| − | + | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/Slide_Wash">iRIf Slide Cleaning</a></li> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | |
| − | <li><a href="https://2015.igem.org/Team:Freiburg/Protocols/ | + | |
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 08:36, 10 September 2015
Protein Purification & Analysis
Cell-free Expression
Surface Chemistry
