Difference between revisions of "Team:Marburg/Protocols"
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<li><a href="https://2015.igem.org/Team:Marburg/Minicells">NUTRInity - Provide</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Minicells">NUTRInity - Provide</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Curli">NUTRInity - Pick up</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li> | + | <li><a href="https://2015.igem.org/Team:Marburg/CDI">NUTRInity - Cut off</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Marburg/Design">Future Application</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/InterLab">InterLab Study</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Measurement">Measurement Study</a></li> | ||
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<li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Hessentag">Hessentag</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Killswitch">KillSwitch Statistics</a></li> | ||
| − | |||
</ul> | </ul> | ||
</li> | </li> | ||
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<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol2">Competent E.Coli cells (Electroporation)</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol2">Competent E.Coli cells (Electroporation)</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol3">Competent E. coli cells (RbCl)</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol3">Competent E. coli cells (RbCl)</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li> | + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol27">Congo Red Staining</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol28">Crystal Violet Staining</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol5">CPEC Standard</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol5">CPEC Standard</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol6">Digests</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol6">Digests</a></li> | ||
| Line 343: | Line 345: | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol9">Gibson Assembly® Master Mix – Assembly</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol9">Gibson Assembly® Master Mix – Assembly</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol10">Glycerol stock generation</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol10">Glycerol stock generation</a></li> | ||
| + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol4">Competent yeast for electroporation</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol11">Macherey-Nagel Plasmid DNA Purification</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol11">Macherey-Nagel Plasmid DNA Purification</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol12">Modified Gel Extraction Protocol</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol12">Modified Gel Extraction Protocol</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/ | + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol29">Ligation</a></li> |
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol14">PCR only with primer</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol14">PCR only with primer</a></li> | ||
| − | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol15">Preparation of Mini Cells</a></li> | + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol15">Preparation of Mini Cells</a></li> |
| + | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol30">SDS-Page</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol16">Transformation into chemocompetent (RbCl) cells</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol16">Transformation into chemocompetent (RbCl) cells</a></li> | ||
<li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol17">Transformation into electrocompetent cells</a></li> | <li><a href="https://2015.igem.org/Team:Marburg/Protocols/Protocol17">Transformation into electrocompetent cells</a></li> | ||
Revision as of 16:06, 16 September 2015
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- Agarose gels
- Competent E.Coli cells (Electroporation)
- Competent E. coli cells (RbCl)
- Competent yeast for electroporation
- Congo Red Staining
- Crystal Violet Staining
- CPEC Standard
- Digests
- Electrocompetent E. coli cells
- GeneJET PCR Purification Kit
- Gibson Assembly® Master Mix – Assembly
- Glycerol stock generation
- Competent yeast for electroporation
- Macherey-Nagel Plasmid DNA Purification
- Modified Gel Extraction Protocol
- Ligation
- PCR only with primer
- Preparation of Mini Cells
- SDS-Page
- Transformation into chemocompetent (RbCl) cells
- Transformation into electrocompetent cells
- Transformation into not competent E.coli cells
- Transformation into yeast
- QIAprep® Spin Miniprep Kit Quick-StartProtocol
- QIAquick® PCR Purification Kit
- Yeast Miniprep
- Zymo DNA Clean & Concentrator™-5
- Zymo Frozen Yeast Tranformation II Kit
- Zyppy™ Plasmid Midiprep Kit
