Difference between revisions of "Team:Paris Bettencourt/Protocols"
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− | + | <div class="textBox" id="HeatShockTransformation"> | |
+ | <h3>Heat Shock Transformation</h3> | ||
+ | <ul> | ||
+ | <li>Thaw frozen chemically competent cells (20µL aliquots) on ice for 10min.</li> | ||
+ | <li>add 2µL of ligation product (or 0.5µL of miniprep) and incubate the cells 30sec at 42°C.</li> | ||
+ | <li>put the cells back on ice for 2min.</li> | ||
+ | <li>add 200µL of LB to the cells and incubate 2 hours at 37°C.</li> | ||
+ | <li>plate the cells on LB + erythromycin (150µg/mL) or LB + erythromycin (10µg/mL), incubation at 37°C overnight.</li> | ||
+ | </ul> | ||
+ | </div> | ||
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<div class="box"> | <div class="box"> | ||
<div class="innerBox yellow"> | <div class="innerBox yellow"> | ||
− | <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols | + | <a href="https://2015.igem.org/Team:Paris_Bettencourt/Protocols#HeatShocktransformation"> |
− | <div class="ptext"><p>Heat Shock | + | <div class="ptext"><p>Heat Shock Transformation</p></div> |
− | <img src=" | + | <img src=""> |
</a> | </a> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2015/2/24/ParisBettencourt_protocolsCentrifuge.JPG"> | <img src="https://static.igem.org/mediawiki/2015/2/24/ParisBettencourt_protocolsCentrifuge.JPG"> | ||
<img src="https://static.igem.org/mediawiki/2015/e/ec/ParisBettencourt_protocolsFrozenEnzymes.JPG"> | <img src="https://static.igem.org/mediawiki/2015/e/ec/ParisBettencourt_protocolsFrozenEnzymes.JPG"> | ||
+ | <img src="https://static.igem.org/mediawiki/2015/0/0d/ParisBettencourt_protocolsBanana.JPG"> | ||
</html> | </html> | ||
{{Paris_Bettencourt/footer}} | {{Paris_Bettencourt/footer}} |
Revision as of 09:14, 11 September 2015