Overview

The ability to image fluorescent stains and proteins is integral to modern biology, but the necessary equipment can be prohibitively expensive, particularly for schools and labs in developing countries. To address this issue, we aim to provide an affordable and well documented fluorescence microscope - easy to build and modify.

The mechanics of our microscope will be 3D printable, and all other parts will be cheap and easy to source. The figure below shows our basic set-up: the sensor and fluorescence cube will move in the Z direction to achieve the necessary focus, whilst the sample will move in the X and Y directions (in order to achieve this translation, we will use Dr Richard Bowman's innovative method, which exploits the flexibility of the 3D printed parts).

As proof-of-concept, we will develop a fluorescent cube for wild-type GFP and one for RFP. They will be interchangeable as necessary, and can be removed for bright-field imaging. Ultimately, we aim to achieve < 10 micron resolution, both in bright-field and fluorescent modes. We are also developing user-friendly software to control the microscope and automate image processing.

We believe that everyone should have access to good education and facilities, regardless of financial status: this is why we want to bring you the best possible low-cost microscope.