Team:Cambridge-JIC/Collaborations

CAMBRIDGE-JIC

Collaborations

Glasgow Team

We can see how you fluoresce

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William and Mary Team

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UK Teams Meetup (Organised by Westminster Team)

Bringing microscopy to iGEMmers

In order for UK iGEM teams to meet, socialise and discuss their projects ahead of the Jamboree, the Westminster iGEM team organised a two day meeting in London. As the Hardware track was new to this year’s competition, and being the only team following this track in the UK, we felt this would be a fantastic opportunity to introduce other teams to this track and to highlight the important role of hardware in synthetic biology.
Our workshop took the form of three stations, each covering a slightly different aspect of our project and each with a different discussion focus:

Optics Bench

A working set-up of our first optics bench, and demonstration 3D printed material. Discussion points:

  • 3D printing and rapid prototyping

  • Principles of open-source hardware

  • Inverting the Raspberry Pi camera lens

  • Field of view and resolution calculation

Microscopy Awaits

A working set-up of our latest microscope prototype, brightfield and fluorescence modes, fully motorised and video streaming to the webshell. Discussion points:

  • Importance of illumination, and difficulty with

  • Remote control over Webshell

  • Epi-cube explanation

  • Stage flex-principle demonstration

Build Your Own

An interactive demonstration on building our microscope from scratch. Discussion points:

  • Importance of documentation and instructions

  • Design choices

  • Discuss improvements required

  • How to use Arduino and Raspberry Pi

The entire team was involved, and this allowed members of other teams to chat and ask questions at all three stations while the equipment was being run. The level of enthusiasm from other team members was extremely encouraging, and we were very surprised by the number of questions about the hardware track in general. We hope that as a result of our worksop, some teams may consider it for next year’s competition.
Perhaps one of the most important outcomes of the workshop was in raising awareness of the role of open source hardware (OSH). We emphasised the degree of collaboration and community that it fosters, in the context of our own project. Underlying this is the ability of other iGEM teams to build, modify and improve our microscope in the spirit of OSH and the competition. We invited and encouraged other team members to look at our designs, tinker with them and think about how they might alter them to fit their specific needs.