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Cloning
Blunt End Ligation
Competent Cells
Cryo Stocks
gBlock Procedure
PCR Template Digest
Protein Purification & Analysis
AmidoBlack
Ni-NTA Bead Regeneration
Buffers for Analysis
Cell Lysis
Desalting of Proetin Solutions (Columns)
DotBlot
Freezing and Thawing Cells
Microwave Destaining
Overexpression
Periplasmic Preparation
Purification
SDS-Page
Blood Serum Preparation
Transfection
Western Blot
Cell-free Expression
Cell-free Expression on PDMS Flow Cell
Surface Chemistry
Plasma Activation
GOPTS Surface
APTES-PDITC Surface
Ni-NTA Surface
HALO Surface (Silane)
Glass Slide Preparation (full)
iRIF Slide Preparation (full)
Spotting
Moulding Flowcells
PDITC Surface on PDMS
iRIf
iRIf Measurement
Protein Stamping
iRIf Slide Cleaning