Difference between revisions of "Team:HKUST-Rice/TXTL"

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<h1>What is cell free system?  </h1>
 
<h1>What is cell free system?  </h1>
 
 
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<p>Cell-free system is a platform for <i>in vitro</i> expression of genes. Cell-free system usually comprise of mixture of molecular machinery which will allow transcription and translation of genes to proteins. Compare with whole cell system, a cell-free system is not an organism, thus it is more versatile and can be applied to different media. </p>
+
<p>Cell-free system is a platform for <i>in vitro</i> expression of genes. Cell-free system usually comprises of a mixture of molecular machinery which will allow transcription and translation of genes to proteins. Compared with the whole cell system, since a cell-free system is not an organism, it is more versatile and can be applied to different media. </p>
 
 
 
</div>
 
</div>
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<p>TX-TL cell free system is a <b>safer choice</b> when applying a transgenic
 
<p>TX-TL cell free system is a <b>safer choice</b> when applying a transgenic
−
devices. Our project mainly due with agriculture business, any contamination of
+
device. Since our project mainly deals with the agriculture business, any contamination of
−
crop could lead to mass food poisoning. Therefore we always put <b
+
crops could lead to mass food poisoning. Therefore, this system addresses the <b
−
>biosafety</b> as our top priority.</p>
+
>biosafety</b> concerns.</p>
 
<p >We realize that applying any
 
<p >We realize that applying any
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living genetically modified organism into the field, either with or without built-in
+
living genetically modified organism into the field- whether or not it has a built-in
 
biosecurity measure, could potentially lead to uncontrolled spreading of the
 
biosecurity measure, could potentially lead to uncontrolled spreading of the
−
GMO. Life will finds a way. In view of this, we have to choose a chassis <b
+
GMO. Life will finds a way. In view of this, we chose the <b> cell-free
−
>without self-replicating ability</b> thus
+
system</b> as chassis as it is non-living and <b
−
we choose the non-living<b> cell-free
+
>does not have any self-replicating ability</b>. </p>
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system</b> as chassis. </p>
+
 
<p>Besides being a safer choice,
 
<p>Besides being a safer choice,
−
cell free system allows a more versatile design. Cell-free system get rid of
+
the cell free system allows a more versatile design. The use of <b>growth media</b> to maintain living organisms is not needed. This features make the cell-free system <b
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the <b>needs of growth medium</b> that is
+
−
used to maintain a living organism. This features make cell-free system <b
+
 
>easy to use</b> and <b
 
>easy to use</b> and <b
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normal'>easy to carry</b>. </p>
+
normal'>easy to transport</b>. </p>
 
 
 
</div>
 
</div>
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<h1>TXTL system in our project.</h1>
 
<h1>TXTL system in our project.</h1>
 
 
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<p>In our design, we plan to add the genetically engineered devices, the plasmid containing our biosensors, on a cell-free system on paper as a medium. Soil water sample will be added to this paper-based cell-free system. The paper-based biosensor will then show the result through color changes of paper. In such a devices, it is easy to use and the result is clearly visible to the user. </p>
+
<p>In our design, we plan to add the genetically engineered devices, the plasmid containing our biosensors, on a cell-free system on paper as a medium. Soil water sample will be added to this paper-based cell-free system. The paper-based biosensor will then show the result through color changes of the paper. In such a device, it is easy to use and the result is clearly visible to the user. </p>
 
 
 
</div>
 
</div>

Revision as of 08:52, 10 September 2015


Cell-Free Paper-Based TXTL System

What is cell free system?

Cell-free system is a platform for in vitro expression of genes. Cell-free system usually comprises of a mixture of molecular machinery which will allow transcription and translation of genes to proteins. Compared with the whole cell system, since a cell-free system is not an organism, it is more versatile and can be applied to different media.

Advantages of cell free system chassis

TX-TL cell free system is a safer choice when applying a transgenic device. Since our project mainly deals with the agriculture business, any contamination of crops could lead to mass food poisoning. Therefore, this system addresses the biosafety concerns.

We realize that applying any living genetically modified organism into the field- whether or not it has a built-in biosecurity measure, could potentially lead to uncontrolled spreading of the GMO. Life will finds a way. In view of this, we chose the cell-free system as chassis as it is non-living and does not have any self-replicating ability.

Besides being a safer choice, the cell free system allows a more versatile design. The use of growth media to maintain living organisms is not needed. This features make the cell-free system easy to use and easy to transport.

TXTL system in our project.

In our design, we plan to add the genetically engineered devices, the plasmid containing our biosensors, on a cell-free system on paper as a medium. Soil water sample will be added to this paper-based cell-free system. The paper-based biosensor will then show the result through color changes of the paper. In such a device, it is easy to use and the result is clearly visible to the user.

Result of this system

Coming real soon!!