Difference between revisions of "Team:UCLA/Notebook/Materials"
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**Beta-sheet content (FTIR) | **Beta-sheet content (FTIR) | ||
| − | *Create | + | *Create films from recombinant silk |
| − | * | + | *Characterize recombinant silk films |
| − | + | **Elastic modulus | |
| − | ** | + | **Yield Stress |
| − | ** | + | |
==Achievements== | ==Achievements== | ||
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*August 19: co-spin with the automated stretcher: | *August 19: co-spin with the automated stretcher: | ||
**[https://2015.igem.org/Team:UCLA/Notebook/Materials/18_August_2015] | **[https://2015.igem.org/Team:UCLA/Notebook/Materials/18_August_2015] | ||
| + | |||
| + | |||
== What to accomplish next == | == What to accomplish next == | ||
| − | * | + | *Spin fibers from the recombinant spider silk dopes |
| − | + | ||
==Protocols== | ==Protocols== | ||
Latest revision as of 03:01, 19 September 2015
Materials Processing Notebook
Goals
- Wet-Spin fibers from mixtures of silkworm silk and recombinant functionalized spider silk
- Wet-Spin fibers from recombinant honeybee silk
- Construct a post-spin stretch apparatus
- Characterize all spun fibers
- Elastic modulus
- Yield stress
- Beta-sheet content (FTIR)
- Create films from recombinant silk
- Characterize recombinant silk films
- Elastic modulus
- Yield Stress
Achievements
- May 13: completed our practice spins on cellulose acetate!
- May 28: we reconstituted silk solution into fibers!
- July 9: preliminary success with co-spinning b.mori silk w/ recombinant Tamura silk-GFP hybrid
- July 14: a long contiguous co-spin:
- August 19: co-spin with the automated stretcher:
What to accomplish next
- Spin fibers from the recombinant spider silk dopes
Protocols
- [Team:UCLA/Notebook/Materials/degumming | Silk Degumming]
- [Team:UCLA/Notebook/Materials/solubilization | Silk Solubilization]
- Dialysis to remove LiBr
- https://2015.igem.org/Team:UCLA/Notebook/Materials/Dialysis_Concentrate Dialysis to concentrate silk solution]
- Wet Spinning
- Using the Instron
- Using the lyophilizer
- Dissolving silk in HFIP
- Quick Reference
Raw notebook entries
| May | ||||||
| M | T | W | T | F | S | S |
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| 25 | 26 | 27 | 28 | 29 | 30 | 31 |
| June | ||||||
| M | T | W | T | F | S | S |
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| 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| 22 | 23 | 24 | 25 | 26 | 27 | 28 |
| 29 | 30 | |||||
| July | ||||||
| M | T | W | T | F | S | S |
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| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | 31 | ||
| August | ||||||
| M | T | W | T | F | S | S |
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| 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| 10 | 11 | 12 | 13 | 14 | 15 | 16 |
| 17 | 18 | 19 | 20 | 21 | 22 | 23 |
| 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| 31 | ||||||
| September | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | 5 | 6 | |
| 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 |
| 28 | 29 | 30 | ||||
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