Difference between revisions of "Team:HKUST-Rice/Safety"

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Revision as of 03:54, 29 August 2015

SAFETY


SAFE ORGANISM

Species name (including strain) Risk Group Source Risks to human Ways for acquiring it Ways of usage
E.coli (DH10B) Group 1 http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf
013/500015_MTR-NALT_EN.pdf
 		It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. We acquired from Professor King Lau Chow's lab in HKUST As a chassis for carryout experiment, as a chassis for molecular cloning
E.coli (TK2240) Group 1 National Institute of Health It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194- As a chassis for carryout experiment related to PkdpF promoter and its mutant
E.coli (BL21 Rosetta) Group 1 National Institute of Health It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Professor Minjie Zhang's lab in HKUST For producing Crude cell extract in For TX-TL cell free system
E.coli (S17)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Silberg Lab in Rice University E. coli used to conjugate with soil bacteria
E.coli (XL-1 EC)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Silberg Lab in Rice University Standard strain used for cloning
E.coli (MG1655)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Silberg Lab in Rice University WT used to conjugate w/ MG1655
E.coli (NEB 10-beta DH10B derivative)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Tabor Lab in Rice University Standard strain used for cloning
E.coli (JW1212-1)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Yale CGSC As a chassis for carryout experiment related to PdusC promoters
Azotobacter
vinelandii (ATCC 12837)
Group 1 http://www.agrian.
com/pdfs/HYTa_0-0-0.5_MSDS.pdf 		It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally. ATCC conjugation of E. coli to soil bacteria
Rhizobium
leguminosarum (ATCC 10004)
Group 1 http://xitebio.ca/pea-&-lentil-inoculant/
PeasRhizo-MSDS.pdf		 There is no known health effects. ATCC conjugation of E. coli to soil bacteria

SAFE PROJECT DESIGN

SAFETY TRAINING COURSES

This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:

For HKUST:
1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management

The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to: http://www.ab.ust.hk/hseo/training.htm

For Rice University:
1. General Laboratory Safety
2. Blood Safety and Blood Borne Pathogens

The link to the laboratory safety training requirements of our institution (Rice University) can be directed to: https://osr.rice.edu/Content.aspx?id=788