Difference between revisions of "Team:HKUST-Rice/Safety"

 
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<div class= "project_superrow">
 
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<div id= "page_title"><h1>SAFETY</h1>
 
<div id= "page_title"><h1>SAFETY</h1>
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</div></div>
 
 
 
 
 
<div class="project_content">
 
<div class="project_content">
 
<div class="project_row"><br>
 
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<h1>SAFE ORGANISM</h1>
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<br><h1>SAFE ORGANISMS</h1>
 
 
 
<table id="safety_table" border="2px" align="center">
 
<table id="safety_table" border="2px" align="center">
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<th>Risk Group</th>
 
<th>Risk Group</th>
 
<th>Source</th>
 
<th>Source</th>
−
<th>Risks to human</th>
+
<th>Human Risks</th>
−
<th>Ways for acquiring it</th>
+
<th>Aquisition</th>
−
<th>Ways of usage</th>
+
<th>Use</th>
 
</tr>
 
</tr>
 
</thead>
 
</thead>
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<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
−
<td><i>E.coli</i> (DH10B)</td>
+
<td width="5%"><i>E. coli</i> <br>(DH10B)</td>
−
<td>Group 1</td>
+
<td width="5%">Group 1</td>
−
<td>
+
<td width="25%">
 
<a href="http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf" target="_blank">
 
<a href="http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf" target="_blank">
−
http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf<br>
+
http://tools.thermofisher.com/content/sfs<br>/msds/2013/500015_MTR-NALT_EN.pdf<br>
 
013/500015_MTR-NALT_EN.pdf<br></a>
 
013/500015_MTR-NALT_EN.pdf<br></a>
 
</td>
 
</td>
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<td style="padding-left:10px">It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td width="35%"">Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
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<td>We acquired from Professor King Lau Chow's lab in HKUST </td>
+
<td width="15%">We acquired from Professor King Lau Chow's lab in HKUST </td>
−
<td>As a chassis for carryout experiment, as a chassis for molecular cloning </td>
+
<td width="15%">As a chassis for experiments and cloning
−
+
 
 
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
−
<td><i>E.coli</i> (TK2240)</td>
+
<td><i>E. coli</i> <br>(TK2240)</td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
 
National Institute of Health
 
National Institute of Health
 
</td>
 
</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
−
<td>Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194-</td>
+
<td>Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of <i>Escherichia coli</i> senses external K<sup>+</sup>. Molecular microbiology, 88(6), 1194-</td>
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<td>As a chassis for carryout experiment related to PkdpF promoter and its mutant</td>
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<td>As a chassis for experiments with <i>P<sub>kdpF</sub></i> and its mutant</td>
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
−
<td><i>E.coli</i> (BL21 Rosetta)</td>
+
<td><i>E. coli</i><br> (BL21 Rosetta)</td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
 
National Institute of Health
 
National Institute of Health
 
</td>
 
</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Acquired from Professor Minjie Zhang's lab in HKUST</td>
 
<td>Acquired from Professor Minjie Zhang's lab in HKUST</td>
−
<td>For producing Crude cell extract in For TX-TL cell free system</td>
+
<td>For producing crude cell extract for TX-TL cell free system</td>
 
 
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
−
<td><i>E.coli</i> (S17) <br></td>
+
<td><i>E. coli</i><br> (S17)<br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
 
U.S. Public Health Service Guidelines
 
U.S. Public Health Service Guidelines
 
</td>
 
</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Acquired from Silberg Lab in Rice University
 
<td>Acquired from Silberg Lab in Rice University
 
</td>
 
</td>
−
<td>E. coli used to conjugate with soil bacteria
+
<td><i>E. coli</i> used to conjugate with soil bacteria
 
</td>
 
</td>
 
 
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
−
<td><i>E.coli</i> (XL-1 EC) <br></td>
+
<td><i>E. coli</i><br> (XL-1 EC) <br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
 
U.S. Public Health Service Guidelines
 
U.S. Public Health Service Guidelines
 
</td>
 
</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Acquired from Silberg Lab in Rice University
 
<td>Acquired from Silberg Lab in Rice University
 
</td>
 
</td>
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<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
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<td><i>E.coli</i> (MG1655) <br></td>
+
<td><i>E. coli</i><br> (MG1655) <br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
 
U.S. Public Health Service Guidelines
 
U.S. Public Health Service Guidelines
 
</td>
 
</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Acquired from Silberg Lab in Rice University
 
<td>Acquired from Silberg Lab in Rice University
 
</td>
 
</td>
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<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
−
<td><i>E.coli</i> (NEB 10-beta DH10B derivative) <br></td>
+
<td><i>E. coli</i><br> (NEB 10-beta <br>DH10B derivative) <br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
 
U.S. Public Health Service Guidelines
 
U.S. Public Health Service Guidelines
 
</td>
 
</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Acquired from Tabor Lab in Rice University
 
<td>Acquired from Tabor Lab in Rice University
 
</td>
 
</td>
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<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
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<td><i>E.coli</i> (JW1212-1) <br></td>
+
<td><i>E. coli</i><br> (JW1212-1) <br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
 
U.S. Public Health Service Guidelines
 
U.S. Public Health Service Guidelines
 
</td>
 
</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Acquired from Yale CGSC
 
<td>Acquired from Yale CGSC
 
</td>
 
</td>
−
<td>As a chassis for carryout experiment related to PdusC promoters</td>
+
<td>As a chassis for experiments related to <i>P<sub>dcuS</sub></i></td>
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
−
<td><i>Azotobacter <br>vinelandii</i> (ATCC 12837) <br></td>
+
<td><i>Azotobacter <br>vinelandii</i><br> (ATCC 12837) <br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
 
<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a>
 
<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a>
 
</td>
 
</td>
−
<td>It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td>
+
<td>May cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td>
 
<td>ATCC
 
<td>ATCC
 
</td>
 
</td>
−
<td>conjugation of E. coli to soil bacteria</td>
+
<td>conjugation of <i>E. coli</i> to soil bacteria</td>
 
 
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
 
<td>
 
<td>
−
<i>Rhizobium <br>leguminosarum</i> (ATCC 10004) <br></td>
+
<i>Rhizobium <br>leguminosarum</i><br> (ATCC 10004) <br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td>
 
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td>
−
<td>There is no known health effects.</td>
+
<td>No known health effects.</td>
 
<td>ATCC
 
<td>ATCC
 
</td>
 
</td>
−
<td>conjugation of E. coli to soil bacteria</td>
+
<td>conjugation of <i>E. coli</i> to soil bacteria</td>
 
</tr></table>
 
</tr></table>
 
</div>
 
</div>
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<hr class="para">
 
<hr class="para">
 
<h1>SAFE PROJECT DESIGN</h1>
 
<h1>SAFE PROJECT DESIGN</h1>
 +
 +
<p>Biological safety is our priority, especially when our focus are related to the agricultural business. Our team does not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.
 +
</p>
 +
<h1>Two Approaches Ensuring Biosafety</h1>
 +
<p>Keeping a biological sample quarantined is the key to maintaining biological safety. As a responsible iGEM team, we will not release any of our genetically modified organism outside the laboratory. In our design, we emphasize keeping the biosensors in the laboratory and bringing a soil sample to the laboratory, instead of putting the biosensors to the field for in situ detection of NPK levels.</p><p>
 +
Using a non-living biosensor is another approach that came to mind. This way, even if the sensor is accidentally released into the field, it will not be able to sustain nor to propagate itself. We opt to use a cell-free transcription-translation system (CF TX-TL) system as the chassis of the non-living biosensors.
 +
 +
</p>
 
</div>
 
</div>
−
<p>Biological safety are our priority, especially when our focus are related to the agricultural business. Our team do not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.
 
−
</p>
 
−
<h1>Two approaches ensuring biosafety</h1>
 
 
 
 
 
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<hr class="para">
 
<hr class="para">
 
<h1>SAFETY TRAINING COURSES</h1>
 
<h1>SAFETY TRAINING COURSES</h1>
−
<p>This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:</p>  
+
<p>This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as follows:</p>  
 
<p style=" font-size: 140%; line-height: 140%;">For HKUST:
 
<p style=" font-size: 140%; line-height: 140%;">For HKUST:
 
 
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<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (Rice University) can be directed to:  
 
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (Rice University) can be directed to:  
−
<a href="https://osr.rice.edu/Content.aspx?id=788" target="_blank">https://osr.rice.edu/Content.aspx?id=788</a></i>
+
<a href="http://safety.rice.edu/Training/Safety_Training_Requirements/" target="_blank">http://safety.rice.edu/Training/Safety_Training_Requirements/</a></i>
−
</p>  
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</p>  
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</html>
 
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{{HKUST-Rice Directory}}
 
 
 
<!--<h2>Safety in iGEM</h2>
 
<!--<h2>Safety in iGEM</h2>

Latest revision as of 04:41, 18 September 2015

SAFETY



SAFE ORGANISMS

Species name (including strain) Risk Group Source Human Risks Aquisition Use
E. coli
(DH10B)		 Group 1 http://tools.thermofisher.com/content/sfs
/msds/2013/500015_MTR-NALT_EN.pdf
013/500015_MTR-NALT_EN.pdf
 		Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. We acquired from Professor King Lau Chow's lab in HKUST As a chassis for experiments and cloning
E. coli
(TK2240)		 Group 1 National Institute of Health Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194- As a chassis for experiments with PkdpF and its mutant
E. coli
(BL21 Rosetta)		 Group 1 National Institute of Health Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Professor Minjie Zhang's lab in HKUST For producing crude cell extract for TX-TL cell free system
E. coli
(S17)
 Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Silberg Lab in Rice University E. coli used to conjugate with soil bacteria
E. coli
(XL-1 EC)
Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Silberg Lab in Rice University Standard strain used for cloning
E. coli
(MG1655)
Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Silberg Lab in Rice University WT used to conjugate w/ MG1655
E. coli
(NEB 10-beta
DH10B derivative)
Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Tabor Lab in Rice University Standard strain used for cloning
E. coli
(JW1212-1)
Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Yale CGSC As a chassis for experiments related to PdcuS
Azotobacter
vinelandii
(ATCC 12837)
Group 1 http://www.agrian.
com/pdfs/HYTa_0-0-0.5_MSDS.pdf 		May cause eye irritation; discomfort and temporary nausea if swallowed accidentally. ATCC conjugation of E. coli to soil bacteria
Rhizobium
leguminosarum
(ATCC 10004)
Group 1 http://xitebio.ca/pea-&-lentil-inoculant/
PeasRhizo-MSDS.pdf		 No known health effects. ATCC conjugation of E. coli to soil bacteria

SAFE PROJECT DESIGN

Biological safety is our priority, especially when our focus are related to the agricultural business. Our team does not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.

Two Approaches Ensuring Biosafety

Keeping a biological sample quarantined is the key to maintaining biological safety. As a responsible iGEM team, we will not release any of our genetically modified organism outside the laboratory. In our design, we emphasize keeping the biosensors in the laboratory and bringing a soil sample to the laboratory, instead of putting the biosensors to the field for in situ detection of NPK levels.

Using a non-living biosensor is another approach that came to mind. This way, even if the sensor is accidentally released into the field, it will not be able to sustain nor to propagate itself. We opt to use a cell-free transcription-translation system (CF TX-TL) system as the chassis of the non-living biosensors.

SAFETY TRAINING COURSES

This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as follows:

For HKUST:
1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management

The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to: http://www.ab.ust.hk/hseo/training.htm

For Rice University:
1. General Laboratory Safety
2. Blood Safety and Blood Borne Pathogens

The link to the laboratory safety training requirements of our institution (Rice University) can be directed to: http://safety.rice.edu/Training/Safety_Training_Requirements/