Difference between revisions of "Team:HKUST-Rice/Safety"
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013/500015_MTR-NALT_EN.pdf<br></a> | 013/500015_MTR-NALT_EN.pdf<br></a> | ||
</td> | </td> | ||
| − | <td width="35%""> | + | <td width="35%"">Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td> |
<td width="15%">We acquired from Professor King Lau Chow's lab in HKUST </td> | <td width="15%">We acquired from Professor King Lau Chow's lab in HKUST </td> | ||
| − | <td width="15%">As a chassis for | + | <td width="15%">As a chassis for experiments and cloning |
| − | + | ||
<tbody style="text-align:center"> | <tbody style="text-align:center"> | ||
<tr> | <tr> | ||
| − | <td><i>E.coli</i> <br>(TK2240)</td> | + | <td><i>E. coli</i> <br>(TK2240)</td> |
<td>Group 1</td> | <td>Group 1</td> | ||
<td> | <td> | ||
National Institute of Health | National Institute of Health | ||
</td> | </td> | ||
| − | <td> | + | <td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td> |
<td>Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of <i>Escherichia coli</i> senses external K<sup>+</sup>. Molecular microbiology, 88(6), 1194-</td> | <td>Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of <i>Escherichia coli</i> senses external K<sup>+</sup>. Molecular microbiology, 88(6), 1194-</td> | ||
| − | <td>As a chassis for | + | <td>As a chassis for experiments with <i>P<sub>kdpF</sub></i> and its mutant</td> |
<tbody style="text-align:center"> | <tbody style="text-align:center"> | ||
<tr> | <tr> | ||
| Line 79: | Line 78: | ||
National Institute of Health | National Institute of Health | ||
</td> | </td> | ||
| − | <td> | + | <td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td> |
<td>Acquired from Professor Minjie Zhang's lab in HKUST</td> | <td>Acquired from Professor Minjie Zhang's lab in HKUST</td> | ||
| − | <td>For producing | + | <td>For producing crude cell extract for TX-TL cell free system</td> |
<tbody style="text-align:center"> | <tbody style="text-align:center"> | ||
| Line 90: | Line 89: | ||
U.S. Public Health Service Guidelines | U.S. Public Health Service Guidelines | ||
</td> | </td> | ||
| − | <td> | + | <td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td> |
<td>Acquired from Silberg Lab in Rice University | <td>Acquired from Silberg Lab in Rice University | ||
</td> | </td> | ||
| Line 98: | Line 97: | ||
<tbody style="text-align:center"> | <tbody style="text-align:center"> | ||
<tr> | <tr> | ||
| − | <td><i>E.coli</i><br> (XL-1 EC) <br></td> | + | <td><i>E. coli</i><br> (XL-1 EC) <br></td> |
<td>Group 1</td> | <td>Group 1</td> | ||
<td> | <td> | ||
U.S. Public Health Service Guidelines | U.S. Public Health Service Guidelines | ||
</td> | </td> | ||
| − | <td> | + | <td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td> |
<td>Acquired from Silberg Lab in Rice University | <td>Acquired from Silberg Lab in Rice University | ||
</td> | </td> | ||
| Line 114: | Line 113: | ||
U.S. Public Health Service Guidelines | U.S. Public Health Service Guidelines | ||
</td> | </td> | ||
| − | <td> | + | <td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td> |
<td>Acquired from Silberg Lab in Rice University | <td>Acquired from Silberg Lab in Rice University | ||
</td> | </td> | ||
| Line 125: | Line 124: | ||
U.S. Public Health Service Guidelines | U.S. Public Health Service Guidelines | ||
</td> | </td> | ||
| − | <td> | + | <td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td> |
<td>Acquired from Tabor Lab in Rice University | <td>Acquired from Tabor Lab in Rice University | ||
</td> | </td> | ||
| Line 136: | Line 135: | ||
U.S. Public Health Service Guidelines | U.S. Public Health Service Guidelines | ||
</td> | </td> | ||
| − | <td> | + | <td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td> |
<td>Acquired from Yale CGSC | <td>Acquired from Yale CGSC | ||
</td> | </td> | ||
| − | <td>As a chassis for | + | <td>As a chassis for experiments related to <i>P<sub>dcuS</sub></i></td> |
<tbody style="text-align:center"> | <tbody style="text-align:center"> | ||
<tr> | <tr> | ||
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<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a> | <a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a> | ||
</td> | </td> | ||
| − | <td> | + | <td>May cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td> |
<td>ATCC | <td>ATCC | ||
</td> | </td> | ||
| Line 158: | Line 157: | ||
<td>Group 1</td> | <td>Group 1</td> | ||
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td> | <td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td> | ||
| − | <td> | + | <td>No known health effects.</td> |
<td>ATCC | <td>ATCC | ||
</td> | </td> | ||
| Line 169: | Line 168: | ||
<h1>SAFE PROJECT DESIGN</h1> | <h1>SAFE PROJECT DESIGN</h1> | ||
| − | <p>Biological safety is our priority, especially when our focus are related to the agricultural business. Our team | + | <p>Biological safety is our priority, especially when our focus are related to the agricultural business. Our team does not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field. |
</p> | </p> | ||
| − | <h1>Two | + | <h1>Two Approaches Ensuring Biosafety</h1> |
| − | <p> | + | <p>Keeping a biological sample quarantined is the key to maintaining biological safety. As a responsible iGEM team, we will not release any of our genetically modified organism outside the laboratory. In our design, we emphasize keeping the biosensors in the laboratory and bringing a soil sample to the laboratory, instead of putting the biosensors to the field for in situ detection of NPK levels.</p><p> |
| − | + | Using a non-living biosensor is another approach that came to mind. This way, even if the sensor is accidentally released into the field, it will not be able to sustain nor to propagate itself. We opt to use a cell-free transcription-translation system (CF TX-TL) system as the chassis of the non-living biosensors. | |
</p> | </p> | ||
Latest revision as of 04:41, 18 September 2015
SAFETY
SAFE ORGANISMS
| Species name (including strain) | Risk Group | Source | Human Risks | Aquisition | Use |
|---|---|---|---|---|---|
| E. coli (DH10B) |
Group 1 |
http://tools.thermofisher.com/content/sfs /msds/2013/500015_MTR-NALT_EN.pdf 013/500015_MTR-NALT_EN.pdf |
Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. | We acquired from Professor King Lau Chow's lab in HKUST | As a chassis for experiments and cloning |
| E. coli (TK2240) |
Group 1 | National Institute of Health | Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. | Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194- | As a chassis for experiments with PkdpF and its mutant |
| E. coli (BL21 Rosetta) |
Group 1 | National Institute of Health | Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. | Acquired from Professor Minjie Zhang's lab in HKUST | For producing crude cell extract for TX-TL cell free system |
| E. coli (S17) |
Group 1 | U.S. Public Health Service Guidelines | Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. | Acquired from Silberg Lab in Rice University | E. coli used to conjugate with soil bacteria |
| E. coli (XL-1 EC) |
Group 1 | U.S. Public Health Service Guidelines | Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. | Acquired from Silberg Lab in Rice University | Standard strain used for cloning |
| E. coli (MG1655) |
Group 1 | U.S. Public Health Service Guidelines | Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. | Acquired from Silberg Lab in Rice University | WT used to conjugate w/ MG1655 |
| E. coli (NEB 10-beta DH10B derivative) |
Group 1 | U.S. Public Health Service Guidelines | Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. | Acquired from Tabor Lab in Rice University | Standard strain used for cloning |
| E. coli (JW1212-1) |
Group 1 | U.S. Public Health Service Guidelines | Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. | Acquired from Yale CGSC | As a chassis for experiments related to PdcuS |
| Azotobacter vinelandii (ATCC 12837) |
Group 1 |
http://www.agrian. com/pdfs/HYTa_0-0-0.5_MSDS.pdf |
May cause eye irritation; discomfort and temporary nausea if swallowed accidentally. | ATCC | conjugation of E. coli to soil bacteria |
|
Rhizobium leguminosarum (ATCC 10004) |
Group 1 | http://xitebio.ca/pea-&-lentil-inoculant/ PeasRhizo-MSDS.pdf |
No known health effects. | ATCC | conjugation of E. coli to soil bacteria |
SAFE PROJECT DESIGN
Biological safety is our priority, especially when our focus are related to the agricultural business. Our team does not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.
Two Approaches Ensuring Biosafety
Keeping a biological sample quarantined is the key to maintaining biological safety. As a responsible iGEM team, we will not release any of our genetically modified organism outside the laboratory. In our design, we emphasize keeping the biosensors in the laboratory and bringing a soil sample to the laboratory, instead of putting the biosensors to the field for in situ detection of NPK levels.
Using a non-living biosensor is another approach that came to mind. This way, even if the sensor is accidentally released into the field, it will not be able to sustain nor to propagate itself. We opt to use a cell-free transcription-translation system (CF TX-TL) system as the chassis of the non-living biosensors.
SAFETY TRAINING COURSES
This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as follows:
For HKUST:
1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management
The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be
directed to: http://www.ab.ust.hk/hseo/training.htm
For Rice University:
1. General Laboratory Safety
2. Blood Safety and Blood Borne Pathogens
The link to the laboratory safety training requirements of our institution (Rice University) can be directed to:
http://safety.rice.edu/Training/Safety_Training_Requirements/