Difference between revisions of "Team:HKUST-Rice/Safety"

 
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<td width="35%"">Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td width="35%"">Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td width="15%">We acquired from Professor King Lau Chow's lab in HKUST </td>
 
<td width="15%">We acquired from Professor King Lau Chow's lab in HKUST </td>
−
<td width="15%">As a chassis for carryout experiment, as a chassis for molecular cloning </td>
+
<td width="15%">As a chassis for experiments and cloning
−
+
 
 
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
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<td><i>E.coli</i> <br>(TK2240)</td>
+
<td><i>E. coli</i> <br>(TK2240)</td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
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<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of <i>Escherichia coli</i> senses external K<sup>+</sup>. Molecular microbiology, 88(6), 1194-</td>
 
<td>Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of <i>Escherichia coli</i> senses external K<sup>+</sup>. Molecular microbiology, 88(6), 1194-</td>
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<td>As a chassis for carryout experiment related to <i>P<sub>kdpF</sub></i> and its mutant</td>
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<td>As a chassis for experiments with <i>P<sub>kdpF</sub></i> and its mutant</td>
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
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<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
 
<td>Acquired from Professor Minjie Zhang's lab in HKUST</td>
 
<td>Acquired from Professor Minjie Zhang's lab in HKUST</td>
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<td>For producing Crude cell extract in For TX-TL cell free system</td>
+
<td>For producing crude cell extract for TX-TL cell free system</td>
 
 
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
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<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
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<td><i>E.coli</i><br> (XL-1 EC) <br></td>
+
<td><i>E. coli</i><br> (XL-1 EC) <br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
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<td>Acquired from Yale CGSC
 
<td>Acquired from Yale CGSC
 
</td>
 
</td>
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<td>As a chassis for carryout experiment related to <i>P<sub>dcuS</sub></i></td>
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<td>As a chassis for experiments related to <i>P<sub>dcuS</sub></i></td>
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
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<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a>
 
<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a>
 
</td>
 
</td>
−
<td>Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
+
<td>May cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td>
 
<td>ATCC
 
<td>ATCC
 
</td>
 
</td>
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<td>Group 1</td>
 
<td>Group 1</td>
 
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td>
 
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td>
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<td>There is no known health effects.</td>
+
<td>No known health effects.</td>
 
<td>ATCC
 
<td>ATCC
 
</td>
 
</td>
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<h1>SAFE PROJECT DESIGN</h1>
 
<h1>SAFE PROJECT DESIGN</h1>
 
 
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<p>Biological safety is our priority, especially when our focus are related to the agricultural business. Our team do not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.
+
<p>Biological safety is our priority, especially when our focus are related to the agricultural business. Our team does not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.
 
</p>
 
</p>
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<h1>Two approaches ensuring biosafety</h1>
+
<h1>Two Approaches Ensuring Biosafety</h1>
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<p>Quarantining of Biological sample is the key to maintain biological safety. As a responsible iGEM team, we will not release any of our genetically modified organism outside the laboratory. In our design, we put emphasis on keeping the biosensors in the laboratory and bring the soil sample to the laboratory, instead of putting the biosensors to the field for <i>in situ</i> detection of ion level.</p>
+
<p>Keeping a biological sample quarantined is the key to maintaining biological safety. As a responsible iGEM team, we will not release any of our genetically modified organism outside the laboratory. In our design, we emphasize keeping the biosensors in the laboratory and bringing a soil sample to the laboratory, instead of putting the biosensors to the field for in situ detection of NPK levels.</p><p>
−
<p>Using non-living biosensor is another approach that came to our mind. Being a non-living sensor, even if the sensor is accidentally leaked to the field, it will not be able to sustain nor to propagate itself. We opt to use cell-free transcription-translation system (CF TX-TL) system as the chassis of the non-living biosensors.  
+
Using a non-living biosensor is another approach that came to mind. This way, even if the sensor is accidentally released into the field, it will not be able to sustain nor to propagate itself. We opt to use a cell-free transcription-translation system (CF TX-TL) system as the chassis of the non-living biosensors.
 
 
 
</p>
 
</p>

Latest revision as of 04:41, 18 September 2015

SAFETY



SAFE ORGANISMS

Species name (including strain) Risk Group Source Human Risks Aquisition Use
E. coli
(DH10B)		 Group 1 http://tools.thermofisher.com/content/sfs
/msds/2013/500015_MTR-NALT_EN.pdf
013/500015_MTR-NALT_EN.pdf
 		Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. We acquired from Professor King Lau Chow's lab in HKUST As a chassis for experiments and cloning
E. coli
(TK2240)		 Group 1 National Institute of Health Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194- As a chassis for experiments with PkdpF and its mutant
E. coli
(BL21 Rosetta)		 Group 1 National Institute of Health Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Professor Minjie Zhang's lab in HKUST For producing crude cell extract for TX-TL cell free system
E. coli
(S17)
 Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Silberg Lab in Rice University E. coli used to conjugate with soil bacteria
E. coli
(XL-1 EC)
Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Silberg Lab in Rice University Standard strain used for cloning
E. coli
(MG1655)
Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Silberg Lab in Rice University WT used to conjugate w/ MG1655
E. coli
(NEB 10-beta
DH10B derivative)
Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Tabor Lab in Rice University Standard strain used for cloning
E. coli
(JW1212-1)
Group 1 U.S. Public Health Service Guidelines Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. Acquired from Yale CGSC As a chassis for experiments related to PdcuS
Azotobacter
vinelandii
(ATCC 12837)
Group 1 http://www.agrian.
com/pdfs/HYTa_0-0-0.5_MSDS.pdf 		May cause eye irritation; discomfort and temporary nausea if swallowed accidentally. ATCC conjugation of E. coli to soil bacteria
Rhizobium
leguminosarum
(ATCC 10004)
Group 1 http://xitebio.ca/pea-&-lentil-inoculant/
PeasRhizo-MSDS.pdf		 No known health effects. ATCC conjugation of E. coli to soil bacteria

SAFE PROJECT DESIGN

Biological safety is our priority, especially when our focus are related to the agricultural business. Our team does not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.

Two Approaches Ensuring Biosafety

Keeping a biological sample quarantined is the key to maintaining biological safety. As a responsible iGEM team, we will not release any of our genetically modified organism outside the laboratory. In our design, we emphasize keeping the biosensors in the laboratory and bringing a soil sample to the laboratory, instead of putting the biosensors to the field for in situ detection of NPK levels.

Using a non-living biosensor is another approach that came to mind. This way, even if the sensor is accidentally released into the field, it will not be able to sustain nor to propagate itself. We opt to use a cell-free transcription-translation system (CF TX-TL) system as the chassis of the non-living biosensors.

SAFETY TRAINING COURSES

This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as follows:

For HKUST:
1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management

The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to: http://www.ab.ust.hk/hseo/training.htm

For Rice University:
1. General Laboratory Safety
2. Blood Safety and Blood Borne Pathogens

The link to the laboratory safety training requirements of our institution (Rice University) can be directed to: http://safety.rice.edu/Training/Safety_Training_Requirements/