Difference between revisions of "Team:UCSF/Project Sub"
(18 intermediate revisions by one other user not shown) | |||
Line 19: | Line 19: | ||
</li> | </li> | ||
− | <li class = " | + | <li class = "projectModeling down"><a href="#pageModeling">MODELING</a> |
− | + | ||
− | + | ||
− | + | ||
</li> | </li> | ||
<li class = "projectResults down"><a href="#pageResults">RESULTS</a> | <li class = "projectResults down"><a href="#pageResults">RESULTS</a> | ||
− | |||
− | |||
− | |||
</li> | </li> | ||
Line 44: | Line 38: | ||
<div class="headerBreak"></div> | <div class="headerBreak"></div> | ||
− | <p class="content1"> | + | <div class="circuitCenter" style="height:250px"> |
− | + | <img src="https://static.igem.org/mediawiki/2015/e/ef/UCSF_Generalized_Circuit_Diagram.png" alt="General Circuit Diagram" width="431" height="250" style="margin:0 0 0 215px;"> | |
− | + | </div> | |
− | + | ||
+ | <p class="content1">We are building a synthetic model in <i>S. cerevisiae</i> to understand communication's role in biological decision-making. Our model consists of four main parts: Basic Circuit, Positive Feedback, Signal Degradation, and Clustering. Using the model, we hope to be able to cause a population divergence with an outside stimulus. | ||
+ | </p> | ||
+ | |||
+ | <p class="content2">To learn more about our synthetic model, click the button below. | ||
</p> | </p> | ||
Line 54: | Line 52: | ||
</div> | </div> | ||
− | + | <div class="navSub navProject"> | |
− | <a id=" | + | <a id="pageModeling"></a> |
<ul> | <ul> | ||
Line 62: | Line 60: | ||
</li> | </li> | ||
− | <li class = " | + | <li class = "projectModelingP"><p>MODELING</p> |
− | + | ||
− | + | ||
− | + | ||
</li> | </li> | ||
<li class = "projectResults down"><a href="#pageResults">RESULTS</a> | <li class = "projectResults down"><a href="#pageResults">RESULTS</a> | ||
− | |||
− | |||
− | |||
</li> | </li> | ||
Line 83: | Line 75: | ||
<div class="main-content"> | <div class="main-content"> | ||
− | <p class="headerProject"> | + | <p class="headerProject">MODELING</p> |
<div class="headerBreak"></div> | <div class="headerBreak"></div> | ||
− | <p class="content1"> | + | <p class="content1"> |
+ | Our project this year is centered around the emergence of distinct community phenotypes when individuals in a population are allowed to communicate. Focusing on the community phenotype of bimodal activation, our model aims to mathematically estimate distinct states of multiple cells in the population in different immediate extracellular environments. | ||
</p> | </p> | ||
− | + | <p class="content2"> | |
− | + | For more information on our modeling, click the button below. | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</p> | </p> | ||
− | <div class="readMore"><a href="https://2015.igem.org/Team:UCSF/ | + | <div class="readMore"><a href="https://2015.igem.org/Team:UCSF/Modeling">Read More <img class="readMoreArrow" src="https://static.igem.org/mediawiki/2015/6/68/UCSF_Read_More_Arrow.png" alt="Next Arrow" height="35" width="35"></a> |
</div> | </div> | ||
Line 144: | Line 100: | ||
</li> | </li> | ||
− | <li class = " | + | <li class = "projectModeling up"><a href="#pageModeling">MODELING</a> |
− | + | ||
− | + | ||
− | + | ||
</li> | </li> | ||
<li class = "projectResultsP"><p>RESULTS</p> | <li class = "projectResultsP"><p>RESULTS</p> | ||
− | |||
− | |||
− | |||
</li> | </li> | ||
Line 169: | Line 119: | ||
<div class="headerBreak"></div> | <div class="headerBreak"></div> | ||
− | <p class="content1"> | + | <p class="content1"> |
+ | Our goal for our circuit is to elicit a divergent community response from two genetically similar cells by varying different communication parameters. To measure distinct community responses in our circuit, we will look at INDIVIDUAL (GFP) response versus COMMUNITY (RFP) response. | ||
+ | </p> | ||
+ | |||
+ | <p class="content2">To learn more about our experiments and results, click the button below. | ||
</p> | </p> | ||
Line 177: | Line 131: | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
<div class="navSub navProject"> | <div class="navSub navProject"> | ||
Line 231: | Line 141: | ||
</li> | </li> | ||
− | <li class = " | + | <li class = "projectModeling up"><a href="#pageModeling">MODELING</a> |
− | + | ||
− | + | ||
− | + | ||
</li> | </li> | ||
<li class = "projectResults up"><a href="#pageResults">RESULTS</a> | <li class = "projectResults up"><a href="#pageResults">RESULTS</a> | ||
− | |||
− | |||
− | |||
</li> | </li> | ||
Line 256: | Line 160: | ||
<div class="headerBreak"></div> | <div class="headerBreak"></div> | ||
− | <p class="content1"> | + | <p class="content1"> |
+ | Our project consisted of different promoters, fluorescent proteins, and many other parts that contributed to our overall circuit. We are making many of these parts available to the public by submitting them to the parts registry. Our team has worked painstakingly throughout the final weeks of the summer to adapt our parts to be BioBrick compatible. By ordering our parts you can recreate 2015 UCSF iGEM project by constructing our circuit. | ||
</p> | </p> | ||
Line 268: | Line 173: | ||
<div><!--```````````````````````````````````````````````START OF FOOTER HTML```````````````````````````````````````````````--> | <div><!--```````````````````````````````````````````````START OF FOOTER HTML```````````````````````````````````````````````--> | ||
<footer> | <footer> | ||
− | + | <div id="backToTop"><a style="text-decoration:none" id="Top"> | |
− | <p class=" | + | <img id="backToTopArrow" src="https://static.igem.org/mediawiki/2015/6/68/UCSF_Read_More_Arrow.png" alt="Back To Top Arrow" width="90" height="90"> |
− | </ | + | </br> |
− | + | <p>BACK TO TOP</p></a> | |
− | < | + | </div> |
− | </ | + | <div id="siteMap"> |
− | + | <ul> | |
+ | <h3 class="hoverHome"><a href="https://2015.igem.org/Team:UCSF">HOME</a></h3> | ||
+ | </ul> | ||
+ | <ul id="hoverProjectSections"> | ||
+ | <h3 class="hoverProject"><a href="https://2015.igem.org/Team:UCSF/Project_Sub">PROJECT</a></h3> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Description">DESCRIPTION</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Modeling">MODELING</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Results">RESULTS</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Parts">PARTS</a></li> | ||
+ | </ul> | ||
+ | <ul id="hoverJudgingSections"> | ||
+ | <h3 class="hoverJudging"><a href="https://2015.igem.org/Team:UCSF/Judging_Sub">JUDGING</a></h3> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Medal_Requirements">MEDAL</br>REQUIREMENTS</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Safety">SAFETY</a></li> | ||
+ | </ul> | ||
+ | <ul id="hoverHumanPracticesSections"> | ||
+ | <h3 class="hoverHumanPractices"><a href="https://2015.igem.org/Team:UCSF/Human_Practices_Sub">HUMAN PRACTICES</a></h3> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Practices">HUMAN PRACTICES</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Wiki_Flicks">WIKI FLICKS</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Collaborations">COLLABORATIONS</a></li> | ||
+ | </ul> | ||
+ | <ul id="hoverNotebooksSections"> | ||
+ | <h3 class="hoverNotebooks"><a href="https://2015.igem.org/Team:UCSF/Notebook_Sub">NOTEBOOKS</a></h3> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Notebook">NOTEBOOKS</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Protocols">PROTOCOLS</a></li> | ||
+ | </ul> | ||
+ | <ul id="hoverTeamSections"> | ||
+ | <h3 class="hoverTeam"><a href="https://2015.igem.org/Team:UCSF/Team_Sub">TEAM</a></h3> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Team">TEAM</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Attributions">ATTRIBUTIONS</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Team#pageTeamAdvisors">ADVISORS</a></li> | ||
+ | <li><a href="https://2015.igem.org/Team:UCSF/Team_Sub#pageSponsors">SPONSORS</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div id="socialMedia"> | ||
+ | <ul> | ||
+ | <li><a href="https://twitter.com/iGEM_UCSF"><img src="https://static.igem.org/mediawiki/2015/3/33/UCSF_Twitter_Logo.png" alt="Twitter Logo" width="30" height="30"> @IGEM_UCSF</a></li> | ||
+ | <li><a href="https://www.facebook.com/pages/UCSF-IGEM/290769630936213"><img src="https://static.igem.org/mediawiki/2015/a/a1/UCSF_Facebook_Logo.png" alt="Twitter Logo" width="30" height="30"> UCSF IGEM</a></li> | ||
+ | <li><a href="mailto:syssynbio@ucsf.edu"><img src="https://static.igem.org/mediawiki/2015/8/82/Email_Logo.png" alt="Email Logo" width="30" height="30"> syssynbio@ucsf.edu</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
</footer> | </footer> | ||
</div><!--````````````````````````````````````````END OF FOOTER HTML```````````````````````````````````````````````````````--> | </div><!--````````````````````````````````````````END OF FOOTER HTML```````````````````````````````````````````````````````--> |
Latest revision as of 02:22, 19 September 2015
DESCRIPTION
We are building a synthetic model in S. cerevisiae to understand communication's role in biological decision-making. Our model consists of four main parts: Basic Circuit, Positive Feedback, Signal Degradation, and Clustering. Using the model, we hope to be able to cause a population divergence with an outside stimulus.
To learn more about our synthetic model, click the button below.
MODELING
Our project this year is centered around the emergence of distinct community phenotypes when individuals in a population are allowed to communicate. Focusing on the community phenotype of bimodal activation, our model aims to mathematically estimate distinct states of multiple cells in the population in different immediate extracellular environments.
For more information on our modeling, click the button below.
RESULTS
Our goal for our circuit is to elicit a divergent community response from two genetically similar cells by varying different communication parameters. To measure distinct community responses in our circuit, we will look at INDIVIDUAL (GFP) response versus COMMUNITY (RFP) response.
To learn more about our experiments and results, click the button below.
PARTS
Our project consisted of different promoters, fluorescent proteins, and many other parts that contributed to our overall circuit. We are making many of these parts available to the public by submitting them to the parts registry. Our team has worked painstakingly throughout the final weeks of the summer to adapt our parts to be BioBrick compatible. By ordering our parts you can recreate 2015 UCSF iGEM project by constructing our circuit.