Potassium Sensor
June
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
July
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
August
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
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Phosphate Sensor
June
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
July
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
August
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
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Nitrate Sensor
June
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
July
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
August
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040
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