Difference between revisions of "Team:HKUST-Rice/Safety"

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<div id= "page_title"><h1>SAFETY</h1>
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<div class="project_content">
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<div class="project_content">
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<div class="project_row"><br>
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<div class="project_row"><br>
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<h1>SAFE ORGANISM</h1>
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<h1>SAFE ORGANISM</h1>
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        <table id="safety_table" border="2px" align="center">
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<thead>
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        <tr>
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−
  <th>Species name (including strain)</th>
+
−
  <th>Risk Group</th>
+
−
    <th>Source</th>
+
−
    <th>Risks to human</th>
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−
    <th>Ways for acquiring it</th>
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−
    <th>Ways of usage</th>
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−
</tr>
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</thead>
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                <tbody style="text-align:center">
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<tr>
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−
<td><i>E.coli</i> (DH10B)</td>
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−
<td>Group 1</td>
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−
<td>
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−
<a href="http://tools.invitrogen.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf" target="_blank">
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−
http://tools.invitrogen<br>
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−
.com/content/sfs/msds/2<br>
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−
013/500015_MTR-NALT_EN.pdf<br></a>
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</td>
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<td style="padding-left:10px">It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
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<td> Prof. King, Chow </td>
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<td>As our chassis.</td>
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−
               
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−
 
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                <tbody style="text-align:center">
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<tr>
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                <td><i>E.coli</i> (TK2240)</td>
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<td>Group 1</td>
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<td>
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−
<a href=#></a>
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−
</td>
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<td></td>
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<td> German Institute</td>
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<td> Used in Potassium sensor experiments.</td>
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−
 
+
−
                <tbody style="text-align:center">
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−
<tr>
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−
<td><i>E.coli</i> (S17) <br></td>
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−
<td>Group 1</td>
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−
<td>
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−
<a href=#></a>
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−
</td>
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−
<td></td>
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−
<td></td>
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<td>Proof-of-concept experiments in application</td>
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−
 
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−
                <tbody style="text-align:center">
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−
<tr>
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−
<td><i>E.coli</i> (XL-1) <br></td>
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−
<td>Group 1</td>
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−
<td>
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−
<a href="https://www.agilent.com/cs/library/msds/200268_NAEnglish.pdf" 
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−
                target="_blank">https://www.agilent.<br>com/cs/library/<br>
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−
                msds/200268_NAEnglish.pdf</a>
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</td>
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−
<td></td>
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−
<td></td>
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<td>Proof-of-concept experiments in application</td>
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−
 
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                <tbody style="text-align:center">
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−
<tr>
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−
<td><i>Azotobacter <br>vinelandii</i> (#) <br></td>
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−
<td>Group 1</td>
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−
<td>
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−
<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a>
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−
</td>
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−
<td>It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td>
+
−
<td></td>
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−
<td>Proof-of-concept experiments in application</td>
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−
 
+
−
                <tbody style="text-align:center">
+
−
<tr>
+
−
<td>
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−
<i>Rhizobium <br>leguminosarum</i> (#) <br></td>
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−
<td>Group 1</td>
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−
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td>
+
−
<td>There is no known health effects.</td>
+
−
<td></td>
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−
<td>Proof-of-concept experiments in application</td>
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−
                </tr></table>
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−
</div>
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−
 
+
−
<div class="project_row">
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−
<hr class="para">
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<h1>SAFE PROJECT DESIGN</h1>
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                </div>
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−
+
−
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−
<div class="project_row">
+
−
<hr class="para">
+
−
<h1>SAFETY TRAINING COURSES</h1>
+
−
<p>This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:</p>
+
−
<p style=" font-size: 140%; line-height: 140%;">For HKUST:
+
−
 
+
−
<br>1. Biological Safety
+
−
<br>2. Chemical Safety I/ Chemical Safety for Laboratory Users
+
−
<br>3. Chemical Safety II/ Hazardous Waste Management
+
−
 
+
−
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be
+
−
directed to: <a href="http://www.ab.ust.hk/hseo/training.htm" target="_blank">http://www.ab.ust.hk/hseo/training.htm</a></i>
+
−
</p>
+
−
 
+
−
<p style=" font-size: 140%; line-height: 140%;">For Rice University:
+
−
 
+
−
<br>1. General Laboratory Safety
+
−
<br>2. Blood Safety and Blood Borne Pathogens
+
−
 
+
−
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (Rice University) can be directed to:
+
−
<a href="https://osr.rice.edu/Content.aspx?id=788" target="_blank">https://osr.rice.edu/Content.aspx?id=788</a></i>
+
−
</p>  
+
 
 
−
                </div>
+
<table id="safety_table" border="2px" align="center">
−
 
+
<thead>
−
</div>
+
<tr>
−
</html>
+
<th>Species name (including strain)</th>
−
 
+
<th>Risk Group</th>
−
 
+
<th>Source</th>
−
<!--<h2>Safety in iGEM</h2>
+
<th>Risks to human</th>
−
 
+
<th>Ways for acquiring it</th>
−
<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
<th>Ways of usage</th>
−
 
+
</tr>
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
</thead>
−
 
+
−
 
+
<tbody style="text-align:center">
−
<h4>Safe Project Design</h4>
+
<tr>
−
 
+
<td><i>E.coli</i> (DH10B)</td>
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
<td>Group 1</td>
−
 
+
<td>
−
<ul>
+
<a href="http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf" target="_blank">
−
<li>Choosing a non-pathogenic chassis</li>
+
http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf<br>
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
013/500015_MTR-NALT_EN.pdf<br></a>
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
</td>
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
<td style="padding-left:10px">It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
−
</ul>
+
<td> We acquired from Professor King Lau Chow's lab in HKUST </td>
−
 
+
<td>as a chassis for carryout experiment, as a chassis for molecular cloning </td>
−
<h4>Safe Lab Work</h4>
+
−
 
+
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
<tbody style="text-align:center">
−
 
+
<tr>
−
<h4>Safe Shipment</h4>
+
<td><i>E.coli</i> (TK2240)</td>
−
 
+
<td>Group 1</td>
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>-->
+
<td>
 +
NIH
 +
</td>
 +
<td></td>
 +
<td> Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194-</td>
 +
<td> As a chassis for carryout experiment related to PkdpF promoter and its mutant</td>
 +
<tbody style="text-align:center">
 +
<tr>
 +
<td><i>E.coli</i> (BL21 Rosetta)</td>
 +
<td>Group 1</td>
 +
<td>
 +
NIH
 +
</td>
 +
<td></td>
 +
<td> Acquired from Professor Minjie Zhang's lab in HKUST</td>
 +
<td> For producing Crude cell extract in For TX-TL cell free system</td>
 +
 +
<tbody style="text-align:center">
 +
<tr>
 +
<td><i>E.coli</i> (S17) <br></td>
 +
<td>Group 1</td>
 +
<td>
 +
U.S. Public Health Service Guidelines
 +
</td>
 +
<td></td>
 +
<td></td>
 +
<td>Proof-of-concept experiments in application</td>
 +
 +
<tbody style="text-align:center">
 +
<tr>
 +
<td><i>E.coli</i> (XL-1 EC) <br></td>
 +
<td>Group 1</td>
 +
<td>
 +
U.S. Public Health Service Guidelines
 +
</td>
 +
<td></td>
 +
<td></td>
 +
<td>Standard strain used for cloning</td>
 +
 +
<tbody style="text-align:center">
 +
<tr>
 +
<td><i>Azotobacter <br>vinelandii</i> (ATCC 12837) <br></td>
 +
<td>Group 1</td>
 +
<td>
 +
<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a>
 +
</td>
 +
<td>It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td>
 +
<td></td>
 +
<td>Proof-of-concept experiments in application</td>
 +
 +
<tbody style="text-align:center">
 +
<tr>
 +
<td>
 +
<i>Rhizobium <br>leguminosarum</i> (ATCC 10004) <br></td>
 +
<td>Group 1</td>
 +
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td>
 +
<td>There is no known health effects.</td>
 +
<td></td>
 +
<td>Proof-of-concept experiments in application</td>
 +
</tr></table>
 +
</div>
 +
 +
<div class="project_row">
 +
<hr class="para">
 +
<h1>SAFE PROJECT DESIGN</h1>
 +
</div>
 +
 +
 +
<div class="project_row">
 +
<hr class="para">
 +
<h1>SAFETY TRAINING COURSES</h1>
 +
<p>This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:</p>
 +
<p style=" font-size: 140%; line-height: 140%;">For HKUST:
 +
 +
<br>1. Biological Safety
 +
<br>2. Chemical Safety I/ Chemical Safety for Laboratory Users
 +
<br>3. Chemical Safety II/ Hazardous Waste Management
 +
 +
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be
 +
directed to: <a href="http://www.ab.ust.hk/hseo/training.htm" target="_blank">http://www.ab.ust.hk/hseo/training.htm</a></i>
 +
</p>
 +
 +
<p style=" font-size: 140%; line-height: 140%;">For Rice University:
 +
 +
<br>1. General Laboratory Safety
 +
<br>2. Blood Safety and Blood Borne Pathogens
 +
 +
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (Rice University) can be directed to:
 +
<a href="https://osr.rice.edu/Content.aspx?id=788" target="_blank">https://osr.rice.edu/Content.aspx?id=788</a></i>
 +
</p>
 +
 +
</div>
 +
 +
</div>
 +
</html>
 +
 +
 +
<!--<h2>Safety in iGEM</h2>
 +
 +
<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 +
 +
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 +
 +
 +
<h4>Safe Project Design</h4>
 +
 +
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 +
 +
<ul>
 +
<li>Choosing a non-pathogenic chassis</li>
 +
<li>Choosing parts that will not harm humans / animals / plants</li>
 +
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 +
<li>Including an "induced lethality" or "kill-switch" device</li>
 +
</ul>
 +
 +
<h4>Safe Lab Work</h4>
 +
 +
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 +
 +
<h4>Safe Shipment</h4>
 +
 +
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>-->

Revision as of 03:37, 29 August 2015

SAFETY


SAFE ORGANISM

Species name (including strain) Risk Group Source Risks to human Ways for acquiring it Ways of usage
E.coli (DH10B) Group 1 http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf
013/500015_MTR-NALT_EN.pdf
 		It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. We acquired from Professor King Lau Chow's lab in HKUST as a chassis for carryout experiment, as a chassis for molecular cloning
E.coli (TK2240) Group 1 NIH Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194- As a chassis for carryout experiment related to PkdpF promoter and its mutant
E.coli (BL21 Rosetta) Group 1 NIH Acquired from Professor Minjie Zhang's lab in HKUST For producing Crude cell extract in For TX-TL cell free system
E.coli (S17)
Group 1 U.S. Public Health Service Guidelines Proof-of-concept experiments in application
E.coli (XL-1 EC)
Group 1 U.S. Public Health Service Guidelines Standard strain used for cloning
Azotobacter
vinelandii (ATCC 12837)
Group 1 http://www.agrian.
com/pdfs/HYTa_0-0-0.5_MSDS.pdf 		It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally. Proof-of-concept experiments in application
Rhizobium
leguminosarum (ATCC 10004)
Group 1 http://xitebio.ca/pea-&-lentil-inoculant/
PeasRhizo-MSDS.pdf		 There is no known health effects. Proof-of-concept experiments in application

SAFE PROJECT DESIGN

SAFETY TRAINING COURSES

This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:

For HKUST:
1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management

The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to: http://www.ab.ust.hk/hseo/training.htm

For Rice University:
1. General Laboratory Safety
2. Blood Safety and Blood Borne Pathogens

The link to the laboratory safety training requirements of our institution (Rice University) can be directed to: https://osr.rice.edu/Content.aspx?id=788