Difference between revisions of "Team:HKUST-Rice/Safety"

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</td>
 
</td>
 
<td>E. coli used to conjugate with soil bacteria
 
<td>E. coli used to conjugate with soil bacteria
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</td>
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</td>
 
 
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
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</td>
 
</td>
 
<td>WT used to conjugate w/ MG1655</td>
 
<td>WT used to conjugate w/ MG1655</td>
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<tbody style="text-align:center">
 +
<tr>
 +
<td><i>E.coli</i> (NEB 10-beta DH10B derivative) <br></td>
 +
<td>Group 1</td>
 +
<td>
 +
U.S. Public Health Service Guidelines
 +
</td>
 +
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
 +
<td>Acquired from Tabor Lab in Rice University
 +
</td>
 +
<td>Standard strain used for cloning</td>
 +
<tbody style="text-align:center">
 +
<tr>
 +
<td><i>E.coli</i> (JW1212-1) <br></td>
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<td>Group 1</td>
 +
<td>
 +
U.S. Public Health Service Guidelines
 +
</td>
 +
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
 +
<td>Acquired from Yale CGSC
 +
</td>
 +
<td>As a chassis for carryout experiment related to PdusC promoters</td>
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
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<td><i>E.coli</i> (NEB 10-beta DH10B derivative) <br></td>
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<td><i>Azotobacter <br>vinelandii</i> (ATCC 12837) <br></td>
 
<td>Group 1</td>
 
<td>Group 1</td>
 
<td>
 
<td>
−
U.S. Public Health Service Guidelines
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<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a>
 
</td>
 
</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td>It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td>
−
<td>Acquired from Tabor Lab in Rice University
+
<td>ATCC
 
</td>
 
</td>
−
<td>Standard strain used for cloning</td>
+
<td>conjugation of E. coli to soil bacteria</td>
 +
 
<tbody style="text-align:center">
 
<tbody style="text-align:center">
 
<tr>
 
<tr>
−
<td><i>E.coli</i> (JW1212-1) <br></td>
 
−
<td>Group 1</td>
 
 
<td>
 
<td>
−
U.S. Public Health Service Guidelines
+
<i>Rhizobium <br>leguminosarum</i> (ATCC 10004) <br></td>
−
</td>
+
<td>Group 1</td>
−
<td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td>
+
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td>
−
<td>Acquired from Yale CGSC
+
<td>There is no known health effects.</td>
 +
<td>ATCC
 
</td>
 
</td>
−
<td>As a chassis for carryout experiment related to PdusC promoters</td>
+
<td>conjugation of E. coli to soil bacteria</td>
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<tbody style="text-align:center">
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</tr></table>
−
<tr>
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</div>
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<td><i>Azotobacter <br>vinelandii</i> (ATCC 12837) <br></td>
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−
<td>Group 1</td>
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<div class="project_row">
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<td>
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<hr class="para">
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<a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a>
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<h1>SAFE PROJECT DESIGN</h1>
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</td>
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</div>
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<td>It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td>
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<p></p>
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<td>ATCC
+
−
</td>
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<div class="project_row">
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<td>conjugation of E. coli to soil bacteria</td>
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<hr class="para">
−
+
<h1>SAFETY TRAINING COURSES</h1>
−
<tbody style="text-align:center">
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<p>This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:</p>  
−
<tr>
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<p style=" font-size: 140%; line-height: 140%;">For HKUST:
−
<td>
+
−
<i>Rhizobium <br>leguminosarum</i> (ATCC 10004) <br></td>
+
−
<td>Group 1</td>
+
−
<td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td>
+
−
<td>There is no known health effects.</td>
+
−
<td>ATCC
+
−
</td>
+
−
<td>conjugation of E. coli to soil bacteria</td>
+
−
</tr></table>
+
−
</div>
+
−
+
−
<div class="project_row">
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−
<hr class="para">
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−
<h1>SAFE PROJECT DESIGN</h1>
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</div>
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<p>Biological safety are our priority, especially when our focus are related to the agricultural business. Our team do not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.
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−
</p>
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<h1>Two approaches ensuring biosafety</h1>
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−
+
−
+
−
<div class="project_row">
+
−
<hr class="para">
+
−
<h1>SAFETY TRAINING COURSES</h1>
+
−
<p>This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:</p>  
+
−
<p style=" font-size: 140%; line-height: 140%;">For HKUST:
+
−
+
−
<br>1. Biological Safety
+
−
<br>2. Chemical Safety I/ Chemical Safety for Laboratory Users
+
−
<br>3. Chemical Safety II/ Hazardous Waste Management
+
−
+
−
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be
+
−
directed to: <a href="http://www.ab.ust.hk/hseo/training.htm" target="_blank">http://www.ab.ust.hk/hseo/training.htm</a></i>
+
−
</p>
+
−
+
−
<p style=" font-size: 140%; line-height: 140%;">For Rice University:
+
−
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<br>1. General Laboratory Safety
+
−
<br>2. Blood Safety and Blood Borne Pathogens
+
−
+
−
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (Rice University) can be directed to:
+
−
<a href="https://osr.rice.edu/Content.aspx?id=788" target="_blank">https://osr.rice.edu/Content.aspx?id=788</a></i>
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−
</p>
+
−
+
−
</div>
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−
+
−
</div>
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−
</html>
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 +
<br>1. Biological Safety
 +
<br>2. Chemical Safety I/ Chemical Safety for Laboratory Users
 +
<br>3. Chemical Safety II/ Hazardous Waste Management
 
 
−
<!--<h2>Safety in iGEM</h2>
+
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be
−
+
directed to: <a href="http://www.ab.ust.hk/hseo/training.htm" target="_blank">http://www.ab.ust.hk/hseo/training.htm</a></i>
−
<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
</p>
−
+
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
<p style=" font-size: 140%; line-height: 140%;">For Rice University:
−
+
−
+
<br>1. General Laboratory Safety
−
<h4>Safe Project Design</h4>
+
<br>2. Blood Safety and Blood Borne Pathogens
−
+
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
<br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (Rice University) can be directed to:
−
+
<a href="https://osr.rice.edu/Content.aspx?id=788" target="_blank">https://osr.rice.edu/Content.aspx?id=788</a></i>
−
<ul>
+
</p>
−
<li>Choosing a non-pathogenic chassis</li>
+
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
</div>
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
</div>
−
</ul>
+
</html>
−
+
−
<h4>Safe Lab Work</h4>
+
−
+
<!--<h2>Safety in iGEM</h2>
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
−
+
<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
−
<h4>Safe Shipment</h4>
+
−
+
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>-->
+
 +
 +
<h4>Safe Project Design</h4>
 +
 +
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 +
 +
<ul>
 +
<li>Choosing a non-pathogenic chassis</li>
 +
<li>Choosing parts that will not harm humans / animals / plants</li>
 +
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 +
<li>Including an "induced lethality" or "kill-switch" device</li>
 +
</ul>
 +
 +
<h4>Safe Lab Work</h4>
 +
 +
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 +
 +
<h4>Safe Shipment</h4>
 +
 +
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>-->

Revision as of 03:57, 29 August 2015

SAFETY


SAFE ORGANISM

Species name (including strain) Risk Group Source Risks to human Ways for acquiring it Ways of usage
E.coli (DH10B) Group 1 http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf
013/500015_MTR-NALT_EN.pdf
 		It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. We acquired from Professor King Lau Chow's lab in HKUST As a chassis for carryout experiment, as a chassis for molecular cloning
E.coli (TK2240) Group 1 National Institute of Health It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194- As a chassis for carryout experiment related to PkdpF promoter and its mutant
E.coli (BL21 Rosetta) Group 1 National Institute of Health It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Professor Minjie Zhang's lab in HKUST For producing Crude cell extract in For TX-TL cell free system
E.coli (S17)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Silberg Lab in Rice University E. coli used to conjugate with soil bacteria
E.coli (XL-1 EC)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Silberg Lab in Rice University Standard strain used for cloning
E.coli (MG1655)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Silberg Lab in Rice University WT used to conjugate w/ MG1655
E.coli (NEB 10-beta DH10B derivative)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Tabor Lab in Rice University Standard strain used for cloning
E.coli (JW1212-1)
Group 1 U.S. Public Health Service Guidelines It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. Acquired from Yale CGSC As a chassis for carryout experiment related to PdusC promoters
Azotobacter
vinelandii (ATCC 12837)
Group 1 http://www.agrian.
com/pdfs/HYTa_0-0-0.5_MSDS.pdf 		It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally. ATCC conjugation of E. coli to soil bacteria
Rhizobium
leguminosarum (ATCC 10004)
Group 1 http://xitebio.ca/pea-&-lentil-inoculant/
PeasRhizo-MSDS.pdf		 There is no known health effects. ATCC conjugation of E. coli to soil bacteria

SAFE PROJECT DESIGN

SAFETY TRAINING COURSES

This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:

For HKUST:
1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management

The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to: http://www.ab.ust.hk/hseo/training.htm

For Rice University:
1. General Laboratory Safety
2. Blood Safety and Blood Borne Pathogens

The link to the laboratory safety training requirements of our institution (Rice University) can be directed to: https://osr.rice.edu/Content.aspx?id=788