Difference between revisions of "Team:HKUST-Rice/Safety"
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<tbody style="text-align:center"> | <tbody style="text-align:center"> | ||
<tr> | <tr> | ||
| − | <td><i> | + | <td><i>E.coli</i> (NEB 10-beta DH10B derivative) <br></td> |
<td>Group 1</td> | <td>Group 1</td> | ||
<td> | <td> | ||
| − | + | U.S. Public Health Service Guidelines | |
</td> | </td> | ||
| − | <td>It may be cause eye | + | <td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td> |
| − | <td> | + | <td>Acquired from Tabor Lab in Rice University |
</td> | </td> | ||
| − | <td> | + | <td>Standard strain used for cloning</td> |
| − | + | ||
<tbody style="text-align:center"> | <tbody style="text-align:center"> | ||
<tr> | <tr> | ||
| + | <td><i>E.coli</i> (JW1212-1) <br></td> | ||
| + | <td>Group 1</td> | ||
<td> | <td> | ||
| − | + | U.S. Public Health Service Guidelines | |
| − | + | </td> | |
| − | <td> | + | <td>It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.</td> |
| − | + | <td>Acquired from Yale CGSC | |
| − | <td> | + | |
</td> | </td> | ||
| − | <td>conjugation of E. coli to soil bacteria</td> | + | <td>As a chassis for carryout experiment related to PdusC promoters</td> |
| − | + | <tbody style="text-align:center"> | |
| − | + | <tr> | |
| − | + | <td><i>Azotobacter <br>vinelandii</i> (ATCC 12837) <br></td> | |
| − | + | <td>Group 1</td> | |
| − | + | <td> | |
| − | + | <a href="http://www.agrian.com/pdfs/HYTa_0-0-0.5_MSDS.pdf" target="_blank">http://www.agrian.<br>com/pdfs/HYTa_0-0-0.5_MSDS.pdf</a> | |
| − | + | </td> | |
| − | + | <td>It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally.</td> | |
| − | + | <td>ATCC | |
| − | + | </td> | |
| − | + | <td>conjugation of E. coli to soil bacteria</td> | |
| − | + | ||
| − | + | <tbody style="text-align:center"> | |
| − | + | <tr> | |
| + | <td> | ||
| + | <i>Rhizobium <br>leguminosarum</i> (ATCC 10004) <br></td> | ||
| + | <td>Group 1</td> | ||
| + | <td><a href="http://xitebio.ca/pea-&-lentil-inoculant/PeasRhizo-MSDS.pdf" target="_blank">http://xitebio.ca/pea-&-lentil-inoculant/<br>PeasRhizo-MSDS.pdf</a></td> | ||
| + | <td>There is no known health effects.</td> | ||
| + | <td>ATCC | ||
| + | </td> | ||
| + | <td>conjugation of E. coli to soil bacteria</td> | ||
| + | </tr></table> | ||
| + | </div> | ||
| + | |||
| + | <div class="project_row"> | ||
| + | <hr class="para"> | ||
| + | <h1>SAFE PROJECT DESIGN</h1> | ||
| + | <p>Biological safety is our priority, especially when our focus are related to the agricultural business. Our team do not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.</p> | ||
| + | <h1>Two approaches ensuring biosafety</h1> | ||
| + | <p>Quantization of Biological sample is the key to maintain biological safety. As a responsible iGEM team, we will not release any of our genetically modified outside the laboratory. In our design, we put emphasis on keeping the biosensors in the laboratory and bring the soil sample to the laboratory, instead of putting the biosensors to the field for in situ detection of ion le</p> | ||
| + | <p>Using non-living biosensor is another approach that came to our mind. Being a non-living sensor, even if the sensor is accidentally leaked to the field, it will not be able to sustain nor to propagate itself. We opt to use cell-free transcription-translation system (CF TX-TL) system based on XXX reference as the chassis of the non-living biosensors. | ||
| + | |||
| + | </p> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="project_row"> | ||
| + | <hr class="para"> | ||
| + | <h1>SAFETY TRAINING COURSES</h1> | ||
| + | <p>This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:</p> | ||
| + | <p style=" font-size: 140%; line-height: 140%;">For HKUST: | ||
| + | |||
| + | <br>1. Biological Safety | ||
| + | <br>2. Chemical Safety I/ Chemical Safety for Laboratory Users | ||
| + | <br>3. Chemical Safety II/ Hazardous Waste Management | ||
| + | |||
| + | <br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be | ||
| + | directed to: <a href="http://www.ab.ust.hk/hseo/training.htm" target="_blank">http://www.ab.ust.hk/hseo/training.htm</a></i> | ||
| + | </p> | ||
| + | |||
| + | <p style=" font-size: 140%; line-height: 140%;">For Rice University: | ||
| + | |||
| + | <br>1. General Laboratory Safety | ||
| + | <br>2. Blood Safety and Blood Borne Pathogens | ||
| + | |||
| + | <br><br> <i style="text-align: center; font-size: 90%; line-height: 110%;">The link to the laboratory safety training requirements of our institution (Rice University) can be directed to: | ||
| + | <a href="https://osr.rice.edu/Content.aspx?id=788" target="_blank">https://osr.rice.edu/Content.aspx?id=788</a></i> | ||
| + | </p> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | </html> | ||
| − | |||
| − | |||
| − | |||
| − | + | <!--<h2>Safety in iGEM</h2> | |
| − | + | ||
| − | + | <p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p> | |
| − | + | ||
| − | + | <p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p> | |
| − | + | ||
| − | + | ||
| − | + | <h4>Safe Project Design</h4> | |
| − | + | ||
| − | + | <p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p> | |
| − | + | ||
| − | + | <ul> | |
| − | + | <li>Choosing a non-pathogenic chassis</li> | |
| − | + | <li>Choosing parts that will not harm humans / animals / plants</li> | |
| − | + | <li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li> | |
| − | + | <li>Including an "induced lethality" or "kill-switch" device</li> | |
| − | + | </ul> | |
| − | + | ||
| − | + | <h4>Safe Lab Work</h4> | |
| − | + | ||
| − | + | <p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p> | |
| − | + | ||
| − | + | <h4>Safe Shipment</h4> | |
| − | + | ||
| − | + | <p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>--> | |
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Revision as of 04:04, 29 August 2015
SAFETY
SAFE ORGANISM
| Species name (including strain) | Risk Group | Source | Risks to human | Ways for acquiring it | Ways of usage |
|---|---|---|---|---|---|
| E.coli (DH10B) | Group 1 |
http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf 013/500015_MTR-NALT_EN.pdf |
It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. | We acquired from Professor King Lau Chow's lab in HKUST | As a chassis for carryout experiment, as a chassis for molecular cloning |
| E.coli (TK2240) | Group 1 | National Institute of Health | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. | Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194- | As a chassis for carryout experiment related to PkdpF promoter and its mutant |
| E.coli (BL21 Rosetta) | Group 1 | National Institute of Health | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. | Acquired from Professor Minjie Zhang's lab in HKUST | For producing Crude cell extract in For TX-TL cell free system |
| E.coli (S17) |
Group 1 | U.S. Public Health Service Guidelines | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. | Acquired from Silberg Lab in Rice University | E. coli used to conjugate with soil bacteria |
| E.coli (XL-1 EC) |
Group 1 | U.S. Public Health Service Guidelines | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. | Acquired from Silberg Lab in Rice University | Standard strain used for cloning |
| E.coli (MG1655) |
Group 1 | U.S. Public Health Service Guidelines | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. | Acquired from Silberg Lab in Rice University | WT used to conjugate w/ MG1655 |
| E.coli (NEB 10-beta DH10B derivative) |
Group 1 | U.S. Public Health Service Guidelines | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. | Acquired from Tabor Lab in Rice University | Standard strain used for cloning |
| E.coli (JW1212-1) |
Group 1 | U.S. Public Health Service Guidelines | It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation. | Acquired from Yale CGSC | As a chassis for carryout experiment related to PdusC promoters |
| Azotobacter vinelandii (ATCC 12837) |
Group 1 |
http://www.agrian. com/pdfs/HYTa_0-0-0.5_MSDS.pdf |
It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally. | ATCC | conjugation of E. coli to soil bacteria |
|
Rhizobium leguminosarum (ATCC 10004) |
Group 1 | http://xitebio.ca/pea-&-lentil-inoculant/ PeasRhizo-MSDS.pdf |
There is no known health effects. | ATCC | conjugation of E. coli to soil bacteria |
SAFE PROJECT DESIGN
Biological safety is our priority, especially when our focus are related to the agricultural business. Our team do not want to release and genetically engineered organism into the field, nor to contaminate crop by directly applying the bacteria into the field.
Two approaches ensuring biosafety
Quantization of Biological sample is the key to maintain biological safety. As a responsible iGEM team, we will not release any of our genetically modified outside the laboratory. In our design, we put emphasis on keeping the biosensors in the laboratory and bring the soil sample to the laboratory, instead of putting the biosensors to the field for in situ detection of ion le
Using non-living biosensor is another approach that came to our mind. Being a non-living sensor, even if the sensor is accidentally leaked to the field, it will not be able to sustain nor to propagate itself. We opt to use cell-free transcription-translation system (CF TX-TL) system based on XXX reference as the chassis of the non-living biosensors.
SAFETY TRAINING COURSES
This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:
For HKUST:
1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management
The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be
directed to: http://www.ab.ust.hk/hseo/training.htm
For Rice University:
1. General Laboratory Safety
2. Blood Safety and Blood Borne Pathogens
The link to the laboratory safety training requirements of our institution (Rice University) can be directed to:
https://osr.rice.edu/Content.aspx?id=788