Difference between revisions of "Team:HKUST-Rice/Modeling"
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} | } | ||
− | # | + | #parameters{ |
− | + | text-align:left; | |
− | + | color:#000000; | |
+ | } | ||
+ | |||
+ | #variables{ | ||
+ | text-align:left; | ||
+ | color:#000000; | ||
} | } | ||
</style> | </style> | ||
Line 125: | Line 130: | ||
</div> | </div> | ||
− | + | <div class="project_row"> | |
<hr class="para"> | <hr class="para"> | ||
+ | <h1>Parameters and Variables list</h1> | ||
+ | <center><table id="parameters"> | ||
+ | <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Parameters:<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: | ||
+ | solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Values:<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:1; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>k<sub>1</sub>: Rate constant of forward | ||
+ | auto-phosphorylation<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.23 1/µM <span >hr</span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:2; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>k<sub>-1</sub>:<sub> </sub>Rate constant of | ||
+ | backward auto-phosphorylation<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.0000051 1/µM <span >hr</span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:3; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>k<sub>2</sub>: Rate constant of forward <span | ||
+ | >phosphyl</span>-group transfer<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.00227 1/µM <span >hr</span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:4; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>k<sub>-2</sub>: Rate constant of backward <span | ||
+ | >phosphyl</span>-group transfer<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.00087 1/µM <span >hr</span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:5; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>K<sub>b</sub>: Dissociation constant (<span | ||
+ | ><i style='mso-bidi-font-style:normal'>KdpE</i></span>-DNA)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.0532 1/µM<sup><o:p></o:p></sup></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:6; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>K<sub>h</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>: Dissociation constant (<span ><i | ||
+ | style='mso-bidi-font-style:normal'>KdpF</i>-KdpE</span> <span >dephosphorylation</span> | ||
+ | system)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.0532 µM (adjustable)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:7; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>k<sup>0</sup><sub>h</sub>: Reference <span | ||
+ | >k<sub>h</sub></span> value<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.00023 1/µM <span >hr</span> | ||
+ | (adjustable)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:8; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>C<sup>0</sup><sub>K+</sub>: Reference | ||
+ | concentration of potassium ions <o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.02mM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:9; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>tr</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>: transcription rate constant<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>10600 hr<sup>-1</sup><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:10; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>z</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>: mRNA degradation rate of <span >kdpFABC</span> | ||
+ | and <span >kdpDE</span> coding region<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>21.74 hr<sup>-1<o:p></o:p></sup></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:11; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>d</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>: degradation rate of <span ><i | ||
+ | style='mso-bidi-font-style:normal'>KdpDE</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.2 hr<sup>-1<o:p></o:p></sup></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:12; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>d,F</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>: degradation of <span ><i | ||
+ | style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>4.8 hr<sup>-1</sup><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:13; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>tlD</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>: translation rate constant of <span | ||
+ | ><i style='mso-bidi-font-style:normal'>KdpD</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>160 hr<sup>-1<o:p></o:p></sup></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:14; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>tlE</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>: translation rate constant of <span | ||
+ | ><i style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>8100 hr<sup>-1<o:p></o:p></sup></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:15; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>tlF</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>: translation rate constant of <span | ||
+ | ><i style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>5.4 hr<sup>-1</sup><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:16; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>u: growth rate of the cell (in K minimal medium, | ||
+ | 2<sup>nd</sup> order)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-font-family:""Helvetica Neue", Helvetica, sans-serif";color:black;mso-fareast-language: | ||
+ | EN-US'>0.275466667 hr<sup>-1<o:p></o:p></sup></span></p> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:#660066;mso-fareast-language:ZH-CN'>(exp. obtained)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:17; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-fareast-language:ZH-CN'>Ψ: basal activity of the promoter</span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.0017<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:18; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;color:black;mso-fareast-language:ZH-CN'>k<sub>deg,GFP,mRNA</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-fareast-language:ZH-CN'>: GFP mRNA degradation rate (1<sup>st</sup> | ||
+ | order)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.0048s<sup>-1 </sup><o:p></o:p></span></p> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>=17.28 /<span >hr</span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:19; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;color:black;mso-fareast-language:ZH-CN'>γ<sub>I</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-fareast-language:ZH-CN'>: Immature GFP degradation rate</span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.7 hr<sup>-1<o:p></o:p></sup></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:20; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;color:black;mso-fareast-language:ZH-CN'>γ<sub>m</sub></span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-fareast-language:ZH-CN'>: Mature GFP degradation rate<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>0.021 hr<sup>-1<o:p></o:p></sup></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:21; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-fareast-language:ZH-CN'>ρ: Immature GFP synthesis rate (</span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-themecolor:text1;mso-fareast-language:ZH-CN'>trans rate</span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-fareast-language:ZH-CN'>, 1<sup>st</sup> order)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-themecolor:text1;mso-fareast-language:ZH-CN'>1440 protein/<span | ||
+ | >hr</span>/mRNA (0.4protein/s/mRNA) <o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:22;mso-yfti-lastrow:yes; '> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | color:black;mso-fareast-language:ZH-CN'>w: GFP maturation rate (1<sup>st</sup> | ||
+ | order)<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '> | ||
+ | <p style='margin-right:-15.25pt'><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language: | ||
+ | ZH-CN'>6.48 hr<sup>-1</sup> <o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </center> | ||
+ | <p class="PICdiscription">Table 1. Parameters list 1. </p> | ||
− | <center>< | + | <center> |
− | + | <table id="parameters"> | |
− | + | <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>Parameters<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: | |
− | + | solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>Values<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | <tr style='mso-yfti-irow:1'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
− | + | padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>ADP concentration<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border-top:none;border-left: | |
− | + | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | |
− | + | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>0.305 <span >mM</span><o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | <tr style='mso-yfti-irow:2'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
− | + | padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>ATP concentration<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border-top:none;border-left: | |
− | + | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | |
− | + | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>3.05 <span >mM</span><o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | <tr style='mso-yfti-irow:3'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
− | + | padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>Initial concentration of mRNA (GFP)<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border-top:none;border-left: | |
− | + | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | |
− | + | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>0/cell<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | <tr style='mso-yfti-irow:4'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
− | + | padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>Concentration of <span ><i | |
− | + | style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border-top:none;border-left: | |
− | + | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | |
− | + | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>10 µM<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | <tr style='mso-yfti-irow:5'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
− | + | padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>Concentration of <span ><i | |
− | + | style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border-top:none;border-left: | |
− | + | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | |
− | + | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>2.6 µM<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | <tr style='mso-yfti-irow:6'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
− | + | padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>Concentration of <span ><i | |
− | + | style='mso-bidi-font-style:normal'>KdpD</i></span><o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border-top:none;border-left: | |
− | + | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | |
− | + | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>2.6 µM<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | <tr style='mso-yfti-irow:7'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
− | + | padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>Concentration of inserted plasmid<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border-top:none;border-left: | |
− | + | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | |
− | + | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>0.0183 µM<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | <tr style='mso-yfti-irow:8;mso-yfti-lastrow:yes'> | |
− | + | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | |
− | + | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | |
− | + | padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>Initial concentration of immature and mature GFP<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | <td valign=top style='width:50%;border-top:none;border-left: | |
− | + | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | |
− | + | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | |
− | + | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | |
− | + | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | |
− | + | mso-fareast-language:ZH-CN'>0 /cell<o:p></o:p></span></p> | |
− | + | </td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | </center> | |
− | + | <p class="PICdiscription">Table 2. Parameters list 2. </p> | |
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+ | <center> | ||
+ | <table id="variables"> | ||
+ | <tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Variables<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: | ||
+ | solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Denotation<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt: | ||
+ | solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Unit<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:1'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpD</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shapetype | ||
+ | id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t" | ||
+ | path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f"> | ||
+ | <v:stroke joinstyle="miter"/> | ||
+ | <v:formulas> | ||
+ | <v:f eqn="if lineDrawn pixelLineWidth 0"/> | ||
+ | <v:f eqn="sum @0 1 0"/> | ||
+ | <v:f eqn="sum 0 0 @1"/> | ||
+ | <v:f eqn="prod @2 1 2"/> | ||
+ | <v:f eqn="prod @3 21600 pixelWidth"/> | ||
+ | <v:f eqn="prod @3 21600 pixelHeight"/> | ||
+ | <v:f eqn="sum @0 0 1"/> | ||
+ | <v:f eqn="prod @6 1 2"/> | ||
+ | <v:f eqn="prod @7 21600 pixelWidth"/> | ||
+ | <v:f eqn="sum @8 21600 0"/> | ||
+ | <v:f eqn="prod @7 21600 pixelHeight"/> | ||
+ | <v:f eqn="sum @10 21600 0"/> | ||
+ | </v:formulas> | ||
+ | <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/> | ||
+ | <o:lock v:ext="edit" aspectratio="t"/> | ||
+ | </v:shapetype><v:shape id="_x0000_i1025" type="#_x0000_t75" style='width:43.2pt; | ||
+ | height:16.3pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image001.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=58 | ||
+ | src="Potassium%20Modeling%20wiki.files/image002.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of <span ><i | ||
+ | style='mso-bidi-font-style:normal'>KdpD</i></span><i style='mso-bidi-font-style: | ||
+ | normal'> </i><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:2'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSup><m:sSupPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style: | ||
+ | normal'><m:ctrlPr></m:ctrlPr></span></m:sSupPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpD</m:r></span></i></m:e><m:sup><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>P</m:r></span></i></m:sup></m:sSup></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:50.7pt;height:16.3pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image003.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img | ||
+ | src="Potassium%20Modeling%20wiki.files/image004.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of phosphorylated <span | ||
+ | ><i style='mso-bidi-font-style:normal'>KdpD</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:3'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSup><m:sSupPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style: | ||
+ | normal'><m:ctrlPr></m:ctrlPr></span></m:sSupPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpE</m:r></span></i></m:e><m:sup><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>P</m:r></span></i></m:sup></m:sSup></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:50.1pt;height:16.3pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image005.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=67 | ||
+ | src="Potassium%20Modeling%20wiki.files/image006.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family: | ||
+ | "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of phosphorylated <span | ||
+ | ><i style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:4'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>ADP</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:37.5pt;height:16.5pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image007.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=50 | ||
+ | src="Potassium%20Modeling%20wiki.files/image008.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of ADP molecules<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>mM</span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:5'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>ATP</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:36pt;height:16.5pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image009.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=48 | ||
+ | src="Potassium%20Modeling%20wiki.files/image010.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of ATP molecules<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>mM</span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:6'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpF</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:42.75pt;height:16.5pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image011.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=57 | ||
+ | src="Potassium%20Modeling%20wiki.files/image012.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of <span ><i | ||
+ | style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:7'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:sSub><m:sSubPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style: | ||
+ | normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i style='mso-bidi-font-style: | ||
+ | normal'><span style='font-size:14.0pt;font-family:"Cambria Math",serif; | ||
+ | mso-fareast-language:ZH-CN'><m:r>k</m:r></span></i></m:e><m:sub><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>3</m:r></span></i></m:sub></m:sSub></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:14.25pt;height:16.5pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image013.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=19 | ||
+ | src="Potassium%20Modeling%20wiki.files/image014.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Rate constant of <span >dephosphorylation</span> | ||
+ | of <span ><i style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p> </o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:8'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:sSub><m:sSubPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style: | ||
+ | normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i style='mso-bidi-font-style: | ||
+ | normal'><span style='font-size:14.0pt;font-family:"Cambria Math",serif; | ||
+ | mso-fareast-language:ZH-CN'><m:r>k</m:r></span></i></m:e><m:sub><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>h</m:r></span></i></m:sub></m:sSub></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:15pt;height:16.5pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image015.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=20 | ||
+ | src="Potassium%20Modeling%20wiki.files/image016.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p> </o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p> </o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:9'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:sSub><m:sSubPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style: | ||
+ | normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i style='mso-bidi-font-style: | ||
+ | normal'><span style='font-size:14.0pt;font-family:"Cambria Math",serif; | ||
+ | mso-fareast-language:ZH-CN'><m:r>c</m:r></span></i></m:e><m:sub><m:sSup><m:sSupPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style: | ||
+ | normal'><m:ctrlPr></m:ctrlPr></span></m:sSupPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>K</m:r></span></i></m:e><m:sup><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>+</m:r></span></i></m:sup></m:sSup></m:sub></m:sSub></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:21.3pt;height:16.3pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image017.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=28 | ||
+ | src="Potassium%20Modeling%20wiki.files/image018.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family: | ||
+ | "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of potassium ions<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span ><span style='font-size:14.0pt; | ||
+ | font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>mM</span></span><span | ||
+ | style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:10'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpE</m:r><m:r>-</m:r><m:r>DNA</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:88.3pt;height:16.3pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image019.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=118 | ||
+ | src="Potassium%20Modeling%20wiki.files/image020.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family: | ||
+ | "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of <span ><i | ||
+ | style='mso-bidi-font-style:normal'>KdpE</i></span> bound promoter <span | ||
+ | >P<sub>kdpF</sub></span><sub><o:p></o:p></sub></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<sub><o:p></o:p></sub></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:11'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style: | ||
+ | normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>DNA</m:r></span></i></m:e><m:sub><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>f</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:45.1pt;height:19.4pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image021.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=60 height=26 | ||
+ | src="Potassium%20Modeling%20wiki.files/image022.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family: | ||
+ | "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of Unbound promoter <span | ||
+ | >P<sub>kdpF</sub></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:12'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic; | ||
+ | mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style: | ||
+ | normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>DNA</m:r></span></i></m:e><m:sub><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>0</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:45pt;height:16.5pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image023.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=60 | ||
+ | src="Potassium%20Modeling%20wiki.files/image024.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family: | ||
+ | "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Total concentration of promoter <span | ||
+ | >P<sub>kdpF</sub></span><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:13'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";mso-bidi-font-family: | ||
+ | Times;font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";mso-bidi-font-family:Times; | ||
+ | font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times; | ||
+ | mso-fareast-language:ZH-CN'><m:r>mRNA</m:r></span></i></m:e><m:sub><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times; | ||
+ | mso-fareast-language:ZH-CN'><m:r>GFP</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
+ | id="_x0000_i1025" type="#_x0000_t75" style='width:69.75pt;height:16.5pt'> | ||
+ | <v:imagedata src="Potassium%20Modeling%20wiki.files/image025.png" o:title="" | ||
+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=93 | ||
+ | src="Potassium%20Modeling%20wiki.files/image026.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family: | ||
+ | "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of mRNA molecules for green | ||
+ | fluorescence protein<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'><o:p> </o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:14'> | ||
+ | <td valign=top style='width:50%;border:solid windowtext 1.0pt; | ||
+ | border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt; | ||
+ | padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc | ||
+ | m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr | ||
+ | m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt; | ||
+ | mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family: | ||
+ | "Cambria Math";mso-hansi-font-family:"Cambria Math";mso-bidi-font-family: | ||
+ | Times;font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span | ||
+ | style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math"; | ||
+ | mso-hansi-font-family:"Cambria Math";mso-bidi-font-family:Times; | ||
+ | font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times; | ||
+ | mso-fareast-language:ZH-CN'><m:r>GFP</m:r></span></i></m:e><m:sub><i | ||
+ | style='mso-bidi-font-style:normal'><span style='font-size: | ||
+ | 14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times; | ||
+ | mso-fareast-language:ZH-CN'><m:r>I</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span | ||
+ | style='font-size:12.0pt;font-family:"Calibri",sans-serif; | ||
+ | mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font: | ||
+ | minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US; | ||
+ | mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape | ||
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+ | chromakey="white"/> | ||
+ | </v:shape><![endif]--><![if !vml]><img width=52 | ||
+ | src="Potassium%20Modeling%20wiki.files/image028.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
+ | style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family: | ||
+ | "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif"; | ||
+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of immature green fluorescent | ||
+ | protein<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
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+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style='mso-yfti-irow:15;mso-yfti-lastrow:yes'> | ||
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+ | src="Potassium%20Modeling%20wiki.files/image030.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span | ||
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+ | mso-fareast-language:ZH-CN'><o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
+ | mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt; | ||
+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>Concentration of mature green fluorescent protein<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | <td valign=top style='width:50%;border-top:none;border-left: | ||
+ | none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt; | ||
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+ | mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'> | ||
+ | <p ><span style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif; | ||
+ | mso-fareast-language:ZH-CN'>µM<o:p></o:p></span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </center> | ||
+ | <p class="PICdiscription">Table 3. Variables list. </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="project_row"> | ||
+ | <hr class="para"> | ||
<h1>Reference:</h1> | <h1>Reference:</h1> | ||
<p><br>Heermann R, Zigann K, Gayer S, Rodriguez-Fernandez M, Banga JR, et al. (2014) Dynamics of an Interactive Network Composed of a Bacterial Two- Component System, a Transporter and K+ as Mediator. PLoS ONE 9(2): e89671. doi:10.1371/journal.pone.0089671 | <p><br>Heermann R, Zigann K, Gayer S, Rodriguez-Fernandez M, Banga JR, et al. (2014) Dynamics of an Interactive Network Composed of a Bacterial Two- Component System, a Transporter and K+ as Mediator. PLoS ONE 9(2): e89671. doi:10.1371/journal.pone.0089671 | ||
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Revision as of 14:41, 3 September 2015
Modeling
Model's Assumption
The effect of the endogenous Kdp system of E.coli was neglected.
In our engineered E.coli, it contains the inserted plasmid of PKdpF plus green fluorescence protein generator (BBa_E0240) in pSB3K3 backbone as well as the endogenous Kdp operon, and both operons have the same promoter, PKdpF. As a matter of fact, titration by the endogenous kdp operon of the transcription inducer, phosphorylated KdpE which binds to PKdpF was expected initially. And the titration of phosphorylated KdpE is anticipated to lower the expression of GFP. However, when the DNA copy number of both endogenous and the inserted operons were determined, it was found that the number of endogenous kdp operon is 10 times smaller than that of the inserted one:
This implied that the number of endogenous promoter PKdpF is ten times smaller than that of the inserted operon and accounts only 8.33% of the total number of promoter PKdpF in the engineered E.coli. Therefore, the titration effect of phosphorylated KdpE becomes insignificant. As a result, the effect of endogenous Kdp system was negligible.
Level of KdpD, KdpE and KdpF were assumed to be constant
In accordance to [Kremling A. 04], in the potassium ions concentration range which we were studying, from 0mM to 0.02 mM, the fluctuation of the concentration KdpF as well as KdpD and KdpE was only within 10 µM and 3 µM respectively. Due to the small fluctuation range compared to the gene expression of GFP reporter, it was reasonable to assume the concentration of KdpD, KdpE and KdpF to be constant in the model.
It was assumed that the initial concentration of mRNA for GFP, immature GFP and mature GFP equal to zero.
It was assumed that all reactions below were in steady state such that:
Equations of the Model:
Phosphorylation of KdpD:
Phosphyl-group Transfer:
Binding of KdpE to promoter PkdpF:
Transcription:
Translation:
Green Fluorescent Protein maturation:
Data point fitting to the model:
The experimental data from FACS were used to fit in the prediction model; then we adapt the unit conversion from [Caitlin C, Jeniffer B 13] to convert GFP per cell fluorescence intensity to concentration of GFP per cell:
Prediction model:
Parameters and Variables list
Parameters: |
Values: |
k1: Rate constant of forward
auto-phosphorylation |
0.23 1/µM hr |
k-1: Rate constant of
backward auto-phosphorylation |
0.0000051 1/µM hr |
k2: Rate constant of forward phosphyl-group transfer |
0.00227 1/µM hr |
k-2: Rate constant of backward phosphyl-group transfer |
0.00087 1/µM hr |
Kb: Dissociation constant (KdpE-DNA) |
0.0532 1/µM |
Kh: Dissociation constant (KdpF-KdpE dephosphorylation
system) |
0.0532 µM (adjustable) |
k0h: Reference kh value |
0.00023 1/µM hr
(adjustable) |
C0K+: Reference
concentration of potassium ions |
0.02mM |
ktr: transcription rate constant |
10600 hr-1 |
kz: mRNA degradation rate of kdpFABC
and kdpDE coding region |
21.74 hr-1 |
kd: degradation rate of KdpDE |
0.2 hr-1 |
kd,F: degradation of KdpF |
4.8 hr-1 |
ktlD: translation rate constant of KdpD |
160 hr-1 |
ktlE: translation rate constant of KdpE |
8100 hr-1 |
ktlF: translation rate constant of KdpF |
5.4 hr-1 |
u: growth rate of the cell (in K minimal medium,
2nd order) |
0.275466667 hr-1 (exp. obtained) |
Ψ: basal activity of the promoter |
0.0017 |
kdeg,GFP,mRNA: GFP mRNA degradation rate (1st
order) |
0.0048s-1 =17.28 /hr |
γI: Immature GFP degradation rate |
0.7 hr-1 |
γm: Mature GFP degradation rate |
0.021 hr-1 |
ρ: Immature GFP synthesis rate (trans rate, 1st order) |
1440 protein/hr/mRNA (0.4protein/s/mRNA) |
w: GFP maturation rate (1st
order) |
6.48 hr-1 |
Table 1. Parameters list 1.
Parameters |
Values |
ADP concentration |
0.305 mM |
ATP concentration |
3.05 mM |
Initial concentration of mRNA (GFP) |
0/cell |
Concentration of KdpF |
10 µM |
Concentration of KdpE |
2.6 µM |
Concentration of KdpD |
2.6 µM |
Concentration of inserted plasmid |
0.0183 µM |
Initial concentration of immature and mature GFP |
0 /cell |
Table 2. Parameters list 2.
Variables |
Denotation |
Unit |
|
Concentration of KdpD |
µM |
|
Concentration of phosphorylated KdpD |
µM |
|
Concentration of phosphorylated KdpE |
µM |
|
Concentration of ADP molecules |
mM |
|
Concentration of ATP molecules |
mM |
|
Concentration of KdpF |
µM |
|
Rate constant of dephosphorylation
of KdpE |
|
|
|
|
|
Concentration of potassium ions |
mM |
|
Concentration of KdpE bound promoter PkdpF |
µM |
|
Concentration of Unbound promoter PkdpF |
µM |
|
Total concentration of promoter PkdpF |
µM |
|
Concentration of mRNA molecules for green
fluorescence protein |
|
|
Concentration of immature green fluorescent
protein |
µM |
|
Concentration of mature green fluorescent protein |
µM |
Table 3. Variables list.
Reference:
Heermann R, Zigann K, Gayer S, Rodriguez-Fernandez M, Banga JR, et al. (2014) Dynamics of an Interactive Network Composed of a Bacterial Two- Component System, a Transporter and K+ as Mediator. PLoS ONE 9(2): e89671. doi:10.1371/journal.pone.0089671
Brewster RC, Jones DL, Phillips R (2012) Tuning Promoter Strength through RNA Polymerase Binding Site Design in Escherichia coli. PLoS Comput Biol 8(12): e1002811. doi:10.1371/journal.pcbi.1002811
Modeling, Simulation and Identification of the Dynamics of K Uptake in E. coli. (2014). Universitätsbibliothek der TU München.
Kelly, Jason et al. “Measuring the activity of BioBrick promoters using an in vivo reference standard.” Journal of Biological Engineering 3.1 (2009): 4.
J. Gayer, Stefan. "Modeling, Simulation and Identification of the Dynamics of K Uptake in E. Coli." Technische Universität München Fachgebiet Für Systembiotechnologie (2013). Print.
Kremling, A., Heermann, R., Centler, F., & Gilles, E. (2004). Analysis of two-component signal transduction by mathematical modeling using the KdpD/KdpE system of Escherichia coli.
Conboy, C., & Braff, J. (2013, May 29). Molecules of Equivalent GFP. Retrieved from http://openwetware.org/wiki/MEG