Difference between revisions of "Team:HKUST-Rice/Modeling"

Line 37: Line 37:
 
}
 
}
  
#Kgraph{
+
#parameters{
width:90%;
+
text-align:left;
height:500px;
+
color:#000000;
 +
}
 +
 
 +
#variables{
 +
text-align:left;
 +
color:#000000;
 
}
 
}
 
     </style>  
 
     </style>  
Line 125: Line 130:
 
</div>
 
</div>
  
                    <div class="project_row">
+
                    <div class="project_row">
 
<hr class="para">
 
<hr class="para">
 +
<h1>Parameters and Variables list</h1>
 +
<center><table id="parameters">
 +
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Parameters:<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Values:<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:1; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>k<sub>1</sub>: Rate constant of forward
 +
  auto-phosphorylation<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.23 1/&micro;M <span  >hr</span><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:2; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>k<sub>-1</sub>:<sub> </sub>Rate constant of
 +
  backward auto-phosphorylation<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.0000051 1/&micro;M <span  >hr</span><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:3; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>k<sub>2</sub>: Rate constant of forward <span
 +
  >phosphyl</span>-group transfer<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.00227 1/&micro;M <span  >hr</span><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:4; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>k<sub>-2</sub>: Rate constant of backward <span
 +
  >phosphyl</span>-group transfer<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.00087 1/&micro;M <span  >hr</span><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:5; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>K<sub>b</sub>: Dissociation constant (<span
 +
  ><i style='mso-bidi-font-style:normal'>KdpE</i></span>-DNA)<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.0532 1/&micro;M<sup><o:p></o:p></sup></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:6; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>K<sub>h</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>: Dissociation constant (<span  ><i
 +
  style='mso-bidi-font-style:normal'>KdpF</i>-KdpE</span> <span  >dephosphorylation</span>
 +
  system)<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.0532 &micro;M (adjustable)<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:7; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>k<sup>0</sup><sub>h</sub>: Reference <span
 +
  >k<sub>h</sub></span> value<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.00023 1/&micro;M <span  >hr</span>
 +
  (adjustable)<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:8; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>C<sup>0</sup><sub>K+</sub>: Reference
 +
  concentration of potassium ions <o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.02mM<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:9; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>tr</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>: transcription rate constant<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>10600 hr<sup>-1</sup><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:10; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>z</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>: mRNA degradation rate of <span  >kdpFABC</span>
 +
  and <span  >kdpDE</span> coding region<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>21.74 hr<sup>-1<o:p></o:p></sup></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:11; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>d</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>: degradation rate of <span  ><i
 +
  style='mso-bidi-font-style:normal'>KdpDE</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.2 hr<sup>-1<o:p></o:p></sup></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:12; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>d,F</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>: degradation of <span  ><i
 +
  style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>4.8 hr<sup>-1</sup><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:13; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>tlD</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>: translation rate constant of <span
 +
  ><i style='mso-bidi-font-style:normal'>KdpD</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>160 hr<sup>-1<o:p></o:p></sup></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:14; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>tlE</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>: translation rate constant of <span
 +
  ><i style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>8100 hr<sup>-1<o:p></o:p></sup></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:15; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>k<sub>tlF</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>: translation rate constant of <span
 +
  ><i style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>5.4 hr<sup>-1</sup><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:16; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>u: growth rate of the cell (in K minimal medium,
 +
  2<sup>nd</sup> order)<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-font-family:""Helvetica Neue", Helvetica, sans-serif";color:black;mso-fareast-language:
 +
  EN-US'>0.275466667 hr<sup>-1<o:p></o:p></sup></span></p>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:#660066;mso-fareast-language:ZH-CN'>(exp. obtained)<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:17; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-fareast-language:ZH-CN'>Ψ: basal activity of the promoter</span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.0017<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:18; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;color:black;mso-fareast-language:ZH-CN'>k<sub>deg,GFP,mRNA</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-fareast-language:ZH-CN'>: GFP mRNA degradation rate (1<sup>st</sup>
 +
  order)<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.0048s<sup>-1 </sup><o:p></o:p></span></p>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>=17.28 /<span  >hr</span><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:19; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;color:black;mso-fareast-language:ZH-CN'>γ<sub>I</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-fareast-language:ZH-CN'>: Immature GFP degradation rate</span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.7 hr<sup>-1<o:p></o:p></sup></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:20; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;color:black;mso-fareast-language:ZH-CN'>γ<sub>m</sub></span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-fareast-language:ZH-CN'>: Mature GFP degradation rate<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>0.021 hr<sup>-1<o:p></o:p></sup></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:21; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-fareast-language:ZH-CN'>ρ: Immature GFP synthesis rate (</span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-themecolor:text1;mso-fareast-language:ZH-CN'>trans rate</span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-fareast-language:ZH-CN'>, 1<sup>st</sup> order)<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-themecolor:text1;mso-fareast-language:ZH-CN'>1440 protein/<span
 +
  >hr</span>/mRNA (0.4protein/s/mRNA) <o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:22;mso-yfti-lastrow:yes; '>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  color:black;mso-fareast-language:ZH-CN'>w: GFP maturation rate (1<sup>st</sup>
 +
  order)<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt; '>
 +
  <p  style='margin-right:-15.25pt'><span 
 +
  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:
 +
  ZH-CN'>6.48 hr<sup>-1</sup> <o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
</center>
 +
<p class="PICdiscription">Table 1. Parameters list 1. </p>
 
 
<center><div style="width:90%">
+
<center>
<table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0 width=652
+
<table id="parameters">
style='width:489.1pt;margin-left:-15.9pt;border-collapse:collapse;border:none;
+
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
mso-border-alt:solid windowtext .5pt;mso-yfti-tbllook:1184;mso-padding-alt:
+
  <td   valign=top style='width:50%;border:solid windowtext 1.0pt;
0cm 5.4pt 0cm 5.4pt'>
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;height:21.6pt'>
+
  <p ><span   style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  mso-fareast-language:ZH-CN'>Parameters<o:p></o:p></span></p>
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  </td>
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  <td   valign=top style='width:50%;border:solid windowtext 1.0pt;
  mso-fareast-language:ZH-CN'>Parameters:<o:p></o:p></span></p>
+
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
  </td>
+
  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
  <td width=316 valign=top style='width:236.7pt;border:solid windowtext 1.0pt;
+
  <p ><span   style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
+
  mso-fareast-language:ZH-CN'>Values<o:p></o:p></span></p>
  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  </td>
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
</tr>
  mso-fareast-language:ZH-CN'>Values:<o:p></o:p></span></p>
+
<tr style='mso-yfti-irow:1'>
  </td>
+
  <td   valign=top style='width:50%;border:solid windowtext 1.0pt;
</tr>
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
<tr style='mso-yfti-irow:1;height:21.6pt'>
+
  padding:0cm 5.4pt 0cm 5.4pt'>
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  <p ><span   style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  mso-fareast-language:ZH-CN'>ADP concentration<o:p></o:p></span></p>
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  </td>
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  <td   valign=top style='width:50%;border-top:none;border-left:
  mso-fareast-language:ZH-CN'>k<sub>1</sub>: Rate constant of forward
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
  auto-phosphorylation<o:p></o:p></span></p>
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
  </td>
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  <p ><span   style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-fareast-language:ZH-CN'>0.305 <span >mM</span><o:p></o:p></span></p>
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  </td>
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
</tr>
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
<tr style='mso-yfti-irow:2'>
  mso-fareast-language:ZH-CN'>0.23 1/&micro;M <span class=SpellE>hr</span><o:p></o:p></span></p>
+
  <td   valign=top style='width:50%;border:solid windowtext 1.0pt;
  </td>
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
</tr>
+
  padding:0cm 5.4pt 0cm 5.4pt'>
<tr style='mso-yfti-irow:2;height:21.6pt'>
+
  <p ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  mso-fareast-language:ZH-CN'>ATP concentration<o:p></o:p></span></p>
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  </td>
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <td  valign=top style='width:50%;border-top:none;border-left:
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
   mso-fareast-language:ZH-CN'>k<sub>-1</sub>:<sub> </sub>Rate constant of
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
  backward auto-phosphorylation<o:p></o:p></span></p>
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
  </td>
+
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  mso-fareast-language:ZH-CN'>3.05 <span >mM</span><o:p></o:p></span></p>
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  </td>
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
</tr>
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
<tr style='mso-yfti-irow:3'>
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  <td   valign=top style='width:50%;border:solid windowtext 1.0pt;
  mso-fareast-language:ZH-CN'>0.0000051 1/&micro;M <span class=SpellE>hr</span><o:p></o:p></span></p>
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
  </td>
+
  padding:0cm 5.4pt 0cm 5.4pt'>
</tr>
+
  <p ><span   style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
<tr style='mso-yfti-irow:3;height:21.6pt'>
+
  mso-fareast-language:ZH-CN'>Initial concentration of mRNA (GFP)<o:p></o:p></span></p>
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  </td>
   border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  <td   valign=top style='width:50%;border-top:none;border-left:
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
  mso-fareast-language:ZH-CN'>k<sub>2</sub>: Rate constant of forward <span
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
  class=SpellE>phosphyl</span>-group transfer<o:p></o:p></span></p>
+
  <p ><span   style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  </td>
+
  mso-fareast-language:ZH-CN'>0/cell<o:p></o:p></span></p>
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  </td>
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
</tr>
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
<tr style='mso-yfti-irow:4'>
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <td   valign=top style='width:50%;border:solid windowtext 1.0pt;
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
  mso-fareast-language:ZH-CN'>0.00227 1/&micro;M <span class=SpellE>hr</span><o:p></o:p></span></p>
+
  padding:0cm 5.4pt 0cm 5.4pt'>
  </td>
+
  <p ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  </tr>
+
  mso-fareast-language:ZH-CN'>Concentration of <span ><i
<tr style='mso-yfti-irow:4;height:21.6pt'>
+
  style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p>
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  </td>
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  <td   valign=top style='width:50%;border-top:none;border-left:
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
   mso-fareast-language:ZH-CN'>k<sub>-2</sub>: Rate constant of backward <span
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
  class=SpellE>phosphyl</span>-group transfer<o:p></o:p></span></p>
+
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  </td>
+
  mso-fareast-language:ZH-CN'>10 &micro;M<o:p></o:p></span></p>
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  </td>
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
</tr>
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
<tr style='mso-yfti-irow:5'>
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
  mso-fareast-language:ZH-CN'>0.00087 1/&micro;M <span class=SpellE>hr</span><o:p></o:p></span></p>
+
  padding:0cm 5.4pt 0cm 5.4pt'>
  </td>
+
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
</tr>
+
  mso-fareast-language:ZH-CN'>Concentration of <span  ><i
<tr style='mso-yfti-irow:5;height:21.6pt'>
+
  style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p>
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  </td>
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  <td   valign=top style='width:50%;border-top:none;border-left:
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
  mso-fareast-language:ZH-CN'>K<sub>b</sub>: Dissociation constant (<span
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
  class=SpellE><i style='mso-bidi-font-style:normal'>KdpE</i></span>-DNA)<o:p></o:p></span></p>
+
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  </td>
+
  mso-fareast-language:ZH-CN'>2.6 &micro;M<o:p></o:p></span></p>
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  </td>
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
</tr>
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
<tr style='mso-yfti-irow:6'>
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <td   valign=top style='width:50%;border:solid windowtext 1.0pt;
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
  mso-fareast-language:ZH-CN'>0.0532 1/&micro;M<sup><o:p></o:p></sup></span></p>
+
  padding:0cm 5.4pt 0cm 5.4pt'>
  </td>
+
  <p ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
</tr>
+
  mso-fareast-language:ZH-CN'>Concentration of <span ><i
<tr style='mso-yfti-irow:6;height:21.6pt'>
+
  style='mso-bidi-font-style:normal'>KdpD</i></span><o:p></o:p></span></p>
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  </td>
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  <td   valign=top style='width:50%;border-top:none;border-left:
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
   font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:ZH-CN'>K<sub>h</sub></span></span><span
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  mso-fareast-language:ZH-CN'>: Dissociation constant (<span class=SpellE><i
+
  mso-fareast-language:ZH-CN'>2.6 &micro;M<o:p></o:p></span></p>
  style='mso-bidi-font-style:normal'>KdpF</i>-KdpE</span> <span class=SpellE>dephosphorylation</span>
+
  </td>
  system)<o:p></o:p></span></p>
+
</tr>
  </td>
+
<tr style='mso-yfti-irow:7'>
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  <td   valign=top style='width:50%;border:solid windowtext 1.0pt;
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt'>
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>Concentration of inserted plasmid<o:p></o:p></span></p>
  mso-fareast-language:ZH-CN'>0.0532 &micro;M (adjustable)<o:p></o:p></span></p>
+
  </td>
  </td>
+
  <td   valign=top style='width:50%;border-top:none;border-left:
  </tr>
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
<tr style='mso-yfti-irow:7;height:21.6pt'>
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  <p ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  mso-fareast-language:ZH-CN'>0.0183 &micro;M<o:p></o:p></span></p>
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  </td>
   mso-fareast-language:ZH-CN'>k<sup>0</sup><sub>h</sub>: Reference <span
+
</tr>
  class=SpellE>k<sub>h</sub></span> value<o:p></o:p></span></p>
+
<tr style='mso-yfti-irow:8;mso-yfti-lastrow:yes'>
  </td>
+
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  padding:0cm 5.4pt 0cm 5.4pt'>
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  mso-fareast-language:ZH-CN'>Initial concentration of immature and mature GFP<o:p></o:p></span></p>
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  </td>
  mso-fareast-language:ZH-CN'>0.00023 1/&micro;M <span class=SpellE>hr</span>
+
  <td  valign=top style='width:50%;border-top:none;border-left:
  (adjustable)<o:p></o:p></span></p>
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
  </td>
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
</tr>
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
<tr style='mso-yfti-irow:8;height:21.6pt'>
+
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  mso-fareast-language:ZH-CN'>0 /cell<o:p></o:p></span></p>
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  </td>
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
</tr>
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
</table>
  mso-fareast-language:ZH-CN'>C<sup>0</sup><sub>K+</sub>: Reference
+
</center>
  concentration of potassium ions <o:p></o:p></span></p>
+
<p class="PICdiscription">Table 2. Parameters list 2. </p>
  </td>
+
   <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>0.02mM<o:p></o:p></span></p>
+
  </td>
+
</tr>
+
<tr style='mso-yfti-irow:9;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
  font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:ZH-CN'>k<sub>tr</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>: transcription rate constant<o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>10600 hr<sup>-1</sup><o:p></o:p></span></p>
+
  </td>
+
  </tr>
+
<tr style='mso-yfti-irow:10;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
   font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:ZH-CN'>k<sub>z</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>: mRNA degradation rate of <span class=SpellE>kdpFABC</span>
+
  and <span class=SpellE>kdpDE</span> coding region<o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>21.74 hr<sup>-1<o:p></o:p></sup></span></p>
+
  </td>
+
  </tr>
+
<tr style='mso-yfti-irow:11;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
  font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:ZH-CN'>k<sub>d</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>: degradation rate of <span class=SpellE><i
+
  style='mso-bidi-font-style:normal'>KdpDE</i></span><o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>0.2 hr<sup>-1<o:p></o:p></sup></span></p>
+
  </td>
+
  </tr>
+
<tr style='mso-yfti-irow:12;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
   font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:ZH-CN'>k<sub>d,F</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>: degradation of <span class=SpellE><i
+
  style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>4.8 hr<sup>-1</sup><o:p></o:p></span></p>
+
  </td>
+
</tr>
+
<tr style='mso-yfti-irow:13;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
   font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:ZH-CN'>k<sub>tlD</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>: translation rate constant of <span
+
  class=SpellE><i style='mso-bidi-font-style:normal'>KdpD</i></span><o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>160 hr<sup>-1<o:p></o:p></sup></span></p>
+
  </td>
+
  </tr>
+
<tr style='mso-yfti-irow:14;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
   font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:ZH-CN'>k<sub>tlE</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>: translation rate constant of <span
+
  class=SpellE><i style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>8100 hr<sup>-1<o:p></o:p></sup></span></p>
+
  </td>
+
</tr>
+
<tr style='mso-yfti-irow:15;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
   font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:ZH-CN'>k<sub>tlF</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>: translation rate constant of <span
+
  class=SpellE><i style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
   mso-fareast-language:ZH-CN'>5.4 hr<sup>-1</sup><o:p></o:p></span></p>
+
  </td>
+
</tr>
+
<tr style='mso-yfti-irow:16;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>u: growth rate of the cell (in K minimal medium,
+
  2<sup>nd</sup> order)<o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-font-family:""Helvetica Neue", Helvetica, sans-serif;";color:black;mso-fareast-language:
+
  EN-US'>0.275466667 hr<sup>-1<o:p></o:p></sup></span></p>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:#660066;mso-fareast-language:ZH-CN'>(exp. obtained)<o:p></o:p></span></p>
+
  </td>
+
</tr>
+
<tr style='mso-yfti-irow:17;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:black;mso-fareast-language:ZH-CN'>Ψ: basal activity of the promoter</span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
+
  </td>
+
   <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>0.0017<o:p></o:p></span></p>
+
  </td>
+
</tr>
+
<tr style='mso-yfti-irow:18;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
  font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;color:black;mso-fareast-language:ZH-CN'>k<sub>deg,GFP,mRNA</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:black;mso-fareast-language:ZH-CN'>: GFP mRNA degradation rate (1<sup>st</sup>
+
  order)<o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>0.0048s<sup>-1 </sup><o:p></o:p></span></p>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>=17.28 /<span class=SpellE>hr</span><o:p></o:p></span></p>
+
  </td>
+
  </tr>
+
<tr style='mso-yfti-irow:19;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
   font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;color:black;mso-fareast-language:ZH-CN'>γ<sub>I</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:black;mso-fareast-language:ZH-CN'>: Immature GFP degradation rate</span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
   none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>0.7 hr<sup>-1<o:p></o:p></sup></span></p>
+
  </td>
+
</tr>
+
<tr style='mso-yfti-irow:20;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span class=SpellE><span lang=EN-US style='font-size:14.0pt;
+
  font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;color:black;mso-fareast-language:ZH-CN'>γ<sub>m</sub></span></span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:black;mso-fareast-language:ZH-CN'>: Mature GFP degradation rate<o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  mso-fareast-language:ZH-CN'>0.021 hr<sup>-1<o:p></o:p></sup></span></p>
+
  </td>
+
  </tr>
+
<tr style='mso-yfti-irow:21;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
   color:black;mso-fareast-language:ZH-CN'>ρ: Immature GFP synthesis rate (</span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:black;mso-themecolor:text1;mso-fareast-language:ZH-CN'>trans rate</span><span
+
  lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:black;mso-fareast-language:ZH-CN'>, 1<sup>st</sup> order)<o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:black;mso-themecolor:text1;mso-fareast-language:ZH-CN'>1440 protein/<span
+
  class=SpellE>hr</span>/mRNA (0.4protein/s/mRNA) <o:p></o:p></span></p>
+
  </td>
+
</tr>
+
<tr style='mso-yfti-irow:22;mso-yfti-lastrow:yes;height:21.6pt'>
+
  <td width=337 valign=top style='width:252.4pt;border:solid windowtext 1.0pt;
+
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
+
  padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal><span lang=EN-US style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;
+
  color:black;mso-fareast-language:ZH-CN'>w: GFP maturation rate (1<sup>st</sup>
+
  order)<o:p></o:p></span></p>
+
  </td>
+
  <td width=316 valign=top style='width:236.7pt;border-top:none;border-left:
+
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
+
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
+
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt;height:21.6pt'>
+
  <p class=MsoNormal style='margin-right:-15.25pt'><span lang=EN-US
+
  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif;",serif;mso-fareast-language:
+
  ZH-CN'>6.48 hr<sup>-1</sup> <o:p></o:p></span></p>
+
  </td>
+
</tr>
+
</table>
+
</div></center>
+
  
 +
<center>
 +
<table id="variables">
 +
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Variables<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Denotation<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Unit<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:1'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i
 +
  style='mso-bidi-font-style:normal'><span  style='font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpD</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shapetype
 +
  id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t"
 +
  path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
 +
  <v:stroke joinstyle="miter"/>
 +
  <v:formulas>
 +
<v:f eqn="if lineDrawn pixelLineWidth 0"/>
 +
<v:f eqn="sum @0 1 0"/>
 +
<v:f eqn="sum 0 0 @1"/>
 +
<v:f eqn="prod @2 1 2"/>
 +
<v:f eqn="prod @3 21600 pixelWidth"/>
 +
<v:f eqn="prod @3 21600 pixelHeight"/>
 +
<v:f eqn="sum @0 0 1"/>
 +
<v:f eqn="prod @6 1 2"/>
 +
<v:f eqn="prod @7 21600 pixelWidth"/>
 +
<v:f eqn="sum @8 21600 0"/>
 +
<v:f eqn="prod @7 21600 pixelHeight"/>
 +
<v:f eqn="sum @10 21600 0"/>
 +
  </v:formulas>
 +
  <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
 +
  <o:lock v:ext="edit" aspectratio="t"/>
 +
  </v:shapetype><v:shape id="_x0000_i1025" type="#_x0000_t75" style='width:43.2pt;
 +
  height:16.3pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image001.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=58 
 +
  src="Potassium%20Modeling%20wiki.files/image002.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of <span  ><i
 +
  style='mso-bidi-font-style:normal'>KdpD</i></span><i style='mso-bidi-font-style:
 +
  normal'> </i><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:2'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSup><m:sSupPr><span
 +
style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style:
 +
normal'><m:ctrlPr></m:ctrlPr></span></m:sSupPr><m:e><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpD</m:r></span></i></m:e><m:sup><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>P</m:r></span></i></m:sup></m:sSup></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:50.7pt;height:16.3pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image003.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img   
 +
  src="Potassium%20Modeling%20wiki.files/image004.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of phosphorylated <span
 +
  ><i style='mso-bidi-font-style:normal'>KdpD</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:3'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSup><m:sSupPr><span
 +
style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style:
 +
normal'><m:ctrlPr></m:ctrlPr></span></m:sSupPr><m:e><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpE</m:r></span></i></m:e><m:sup><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>P</m:r></span></i></m:sup></m:sSup></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:50.1pt;height:16.3pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image005.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=67 
 +
  src="Potassium%20Modeling%20wiki.files/image006.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family:
 +
  "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of phosphorylated <span
 +
  ><i style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:4'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i
 +
  style='mso-bidi-font-style:normal'><span  style='font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>ADP</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:37.5pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image007.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=50 
 +
  src="Potassium%20Modeling%20wiki.files/image008.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of ADP molecules<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>mM</span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:5'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i
 +
  style='mso-bidi-font-style:normal'><span  style='font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>ATP</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:36pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image009.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=48 
 +
  src="Potassium%20Modeling%20wiki.files/image010.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of ATP molecules<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>mM</span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:6'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i
 +
  style='mso-bidi-font-style:normal'><span  style='font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpF</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:42.75pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image011.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=57 
 +
  src="Potassium%20Modeling%20wiki.files/image012.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of <span  ><i
 +
  style='mso-bidi-font-style:normal'>KdpF</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:7'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:sSub><m:sSubPr><span
 +
  style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
  mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style:
 +
  normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i style='mso-bidi-font-style:
 +
  normal'><span  style='font-size:14.0pt;font-family:"Cambria Math",serif;
 +
  mso-fareast-language:ZH-CN'><m:r>k</m:r></span></i></m:e><m:sub><i
 +
  style='mso-bidi-font-style:normal'><span  style='font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>3</m:r></span></i></m:sub></m:sSub></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:14.25pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image013.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=19 
 +
  src="Potassium%20Modeling%20wiki.files/image014.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Rate constant of <span  >dephosphorylation</span>
 +
  of <span  ><i style='mso-bidi-font-style:normal'>KdpE</i></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:8'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:sSub><m:sSubPr><span
 +
  style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
  mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style:
 +
  normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i style='mso-bidi-font-style:
 +
  normal'><span  style='font-size:14.0pt;font-family:"Cambria Math",serif;
 +
  mso-fareast-language:ZH-CN'><m:r>k</m:r></span></i></m:e><m:sub><i
 +
  style='mso-bidi-font-style:normal'><span  style='font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>h</m:r></span></i></m:sub></m:sSub></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:15pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image015.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=20 
 +
  src="Potassium%20Modeling%20wiki.files/image016.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:9'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:sSub><m:sSubPr><span
 +
  style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
  mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style:
 +
  normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i style='mso-bidi-font-style:
 +
  normal'><span  style='font-size:14.0pt;font-family:"Cambria Math",serif;
 +
  mso-fareast-language:ZH-CN'><m:r>c</m:r></span></i></m:e><m:sub><m:sSup><m:sSupPr><span
 +
style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style:
 +
normal'><m:ctrlPr></m:ctrlPr></span></m:sSupPr><m:e><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>K</m:r></span></i></m:e><m:sup><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>+</m:r></span></i></m:sup></m:sSup></m:sub></m:sSub></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:21.3pt;height:16.3pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image017.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=28 
 +
  src="Potassium%20Modeling%20wiki.files/image018.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family:
 +
  "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of potassium ions<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  ><span  style='font-size:14.0pt;
 +
  font-family:""Helvetica Neue", Helvetica, sans-serif",serif;mso-fareast-language:ZH-CN'>mM</span></span><span
 +
style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:10'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><i
 +
  style='mso-bidi-font-style:normal'><span  style='font-size:
 +
  14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>kdpE</m:r><m:r>-</m:r><m:r>DNA</m:r></span></i></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:88.3pt;height:16.3pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image019.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=118 
 +
  src="Potassium%20Modeling%20wiki.files/image020.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family:
 +
  "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of <span  ><i
 +
  style='mso-bidi-font-style:normal'>KdpE</i></span> bound promoter <span
 +
  >P<sub>kdpF</sub></span><sub><o:p></o:p></sub></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<sub><o:p></o:p></sub></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:11'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span
 +
style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style:
 +
normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>DNA</m:r></span></i></m:e><m:sub><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>f</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:45.1pt;height:19.4pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image021.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=60 height=26
 +
  src="Potassium%20Modeling%20wiki.files/image022.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family:
 +
  "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of Unbound promoter <span
 +
  >P<sub>kdpF</sub></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:12'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";font-style:italic;
 +
  mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span
 +
style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
mso-hansi-font-family:"Cambria Math";font-style:italic;mso-bidi-font-style:
 +
normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>DNA</m:r></span></i></m:e><m:sub><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-fareast-language:ZH-CN'><m:r>0</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:45pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image023.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=60 
 +
  src="Potassium%20Modeling%20wiki.files/image024.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family:
 +
  "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Total concentration of promoter <span
 +
  >P<sub>kdpF</sub></span><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:13'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";mso-bidi-font-family:
 +
  Times;font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span
 +
style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
mso-hansi-font-family:"Cambria Math";mso-bidi-font-family:Times;
 +
font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times;
 +
mso-fareast-language:ZH-CN'><m:r>mRNA</m:r></span></i></m:e><m:sub><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times;
 +
mso-fareast-language:ZH-CN'><m:r>GFP</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:69.75pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image025.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=93 
 +
  src="Potassium%20Modeling%20wiki.files/image026.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family:
 +
  "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of mRNA molecules for green
 +
  fluorescence protein<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:14'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";mso-bidi-font-family:
 +
  Times;font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span
 +
style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
mso-hansi-font-family:"Cambria Math";mso-bidi-font-family:Times;
 +
font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times;
 +
mso-fareast-language:ZH-CN'><m:r>GFP</m:r></span></i></m:e><m:sub><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times;
 +
mso-fareast-language:ZH-CN'><m:r>I</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:39pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image027.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=52 
 +
  src="Potassium%20Modeling%20wiki.files/image028.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family:
 +
  "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of immature green fluorescent
 +
  protein<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:15;mso-yfti-lastrow:yes'>
 +
  <td  valign=top style='width:50%;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><!--[if gte msEquation 12]><m:oMathPara><m:oMathParaPr><m:jc
 +
m:val="left"/></m:oMathParaPr><m:oMath><m:d><m:dPr><m:begChr m:val="["/><m:endChr
 +
  m:val="]"/><span style='font-size:14.0pt;mso-ansi-font-size:14.0pt;
 +
  mso-bidi-font-size:14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:
 +
  "Cambria Math";mso-hansi-font-family:"Cambria Math";mso-bidi-font-family:
 +
  Times;font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:dPr><m:e><m:sSub><m:sSubPr><span
 +
style='font-size:14.0pt;mso-ansi-font-size:14.0pt;mso-bidi-font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-ascii-font-family:"Cambria Math";
 +
mso-hansi-font-family:"Cambria Math";mso-bidi-font-family:Times;
 +
font-style:italic;mso-bidi-font-style:normal'><m:ctrlPr></m:ctrlPr></span></m:sSubPr><m:e><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times;
 +
mso-fareast-language:ZH-CN'><m:r>GFP</m:r></span></i></m:e><m:sub><i
 +
style='mso-bidi-font-style:normal'><span  style='font-size:
 +
14.0pt;font-family:"Cambria Math",serif;mso-bidi-font-family:Times;
 +
mso-fareast-language:ZH-CN'><m:r>M</m:r></span></i></m:sub></m:sSub></m:e></m:d></m:oMath></m:oMathPara><![endif]--><![if !msEquation]><span
 +
style='font-size:12.0pt;font-family:"Calibri",sans-serif;
 +
  mso-ascii-theme-font:minor-latin;mso-fareast-font-family:宋体;mso-fareast-theme-font:
 +
  minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ansi-language:EN-US;
 +
  mso-fareast-language:ZH-TW;mso-bidi-language:AR-SA'><!--[if gte vml 1]><v:shape
 +
  id="_x0000_i1025" type="#_x0000_t75" style='width:44.25pt;height:16.5pt'>
 +
  <v:imagedata src="Potassium%20Modeling%20wiki.files/image029.png" o:title=""
 +
chromakey="white"/>
 +
  </v:shape><![endif]--><![if !vml]><img
 +
  src="Potassium%20Modeling%20wiki.files/image030.png" v:shapes="_x0000_i1025"><![endif]></span><![endif]><span
 +
style='font-size:14.0pt;mso-ascii-font-family:Cambria;mso-fareast-font-family:
 +
  "MS Mincho";mso-hansi-font-family:Cambria;mso-bidi-font-family:""Helvetica Neue", Helvetica, sans-serif";
 +
  mso-fareast-language:ZH-CN'><o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>Concentration of mature green fluorescent protein<o:p></o:p></span></p>
 +
  </td>
 +
  <td  valign=top style='width:50%;border-top:none;border-left:
 +
  none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0cm 5.4pt 0cm 5.4pt'>
 +
  <p  ><span  style='font-size:14.0pt;font-family:""Helvetica Neue", Helvetica, sans-serif",serif;
 +
  mso-fareast-language:ZH-CN'>&micro;M<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
</center>
 +
<p class="PICdiscription">Table 3. Variables list. </p>
 
 
 +
</div>
 +
 +
                    <div class="project_row">
 +
<hr class="para">
 
<h1>Reference:</h1>
 
<h1>Reference:</h1>
 
<p><br>Heermann R, Zigann K, Gayer S, Rodriguez-Fernandez M, Banga JR, et al. (2014) Dynamics of an Interactive Network Composed of a Bacterial Two- Component System, a Transporter and K+ as Mediator. PLoS ONE 9(2): e89671. doi:10.1371/journal.pone.0089671  
 
<p><br>Heermann R, Zigann K, Gayer S, Rodriguez-Fernandez M, Banga JR, et al. (2014) Dynamics of an Interactive Network Composed of a Bacterial Two- Component System, a Transporter and K+ as Mediator. PLoS ONE 9(2): e89671. doi:10.1371/journal.pone.0089671  
Line 552: Line 1,430:
 
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Revision as of 14:41, 3 September 2015

Modeling

Model's Assumption

The effect of the endogenous Kdp system of E.coli was neglected.

In our engineered E.coli, it contains the inserted plasmid of PKdpF plus green fluorescence protein generator (BBa_E0240) in pSB3K3 backbone as well as the endogenous Kdp operon, and both operons have the same promoter, PKdpF. As a matter of fact, titration by the endogenous kdp operon of the transcription inducer, phosphorylated KdpE which binds to PKdpF was expected initially. And the titration of phosphorylated KdpE is anticipated to lower the expression of GFP. However, when the DNA copy number of both endogenous and the inserted operons were determined, it was found that the number of endogenous kdp operon is 10 times smaller than that of the inserted one:

image caption

This implied that the number of endogenous promoter PKdpF is ten times smaller than that of the inserted operon and accounts only 8.33% of the total number of promoter PKdpF in the engineered E.coli. Therefore, the titration effect of phosphorylated KdpE becomes insignificant. As a result, the effect of endogenous Kdp system was negligible.

Level of KdpD, KdpE and KdpF were assumed to be constant

In accordance to [Kremling A. 04], in the potassium ions concentration range which we were studying, from 0mM to 0.02 mM, the fluctuation of the concentration KdpF as well as KdpD and KdpE was only within 10 µM and 3 µM respectively. Due to the small fluctuation range compared to the gene expression of GFP reporter, it was reasonable to assume the concentration of KdpD, KdpE and KdpF to be constant in the model.

It was assumed that the initial concentration of mRNA for GFP, immature GFP and mature GFP equal to zero.

It was assumed that all reactions below were in steady state such that:

image caption

Equations of the Model:

Phosphorylation of KdpD:

image caption

Phosphyl-group Transfer:

image caption

Binding of KdpE to promoter PkdpF:

image caption

Transcription:

image caption

Translation:

image caption

Green Fluorescent Protein maturation:

image caption

Data point fitting to the model:

The experimental data from FACS were used to fit in the prediction model; then we adapt the unit conversion from [Caitlin C, Jeniffer B 13] to convert GFP per cell fluorescence intensity to concentration of GFP per cell:

image caption

Prediction model:

image caption

Parameters and Variables list

Parameters:

Values:

k1: Rate constant of forward auto-phosphorylation

0.23 1/µM hr

k-1: Rate constant of backward auto-phosphorylation

0.0000051 1/µM hr

k2: Rate constant of forward phosphyl-group transfer

0.00227 1/µM hr

k-2: Rate constant of backward phosphyl-group transfer

0.00087 1/µM hr

Kb: Dissociation constant (KdpE-DNA)

0.0532 1/µM

Kh: Dissociation constant (KdpF-KdpE dephosphorylation system)

0.0532 µM (adjustable)

k0h: Reference kh value

0.00023 1/µM hr (adjustable)

C0K+: Reference concentration of potassium ions

0.02mM

ktr: transcription rate constant

10600 hr-1

kz: mRNA degradation rate of kdpFABC and kdpDE coding region

21.74 hr-1

kd: degradation rate of KdpDE

0.2 hr-1

kd,F: degradation of KdpF

4.8 hr-1

ktlD: translation rate constant of KdpD

160 hr-1

ktlE: translation rate constant of KdpE

8100 hr-1

ktlF: translation rate constant of KdpF

5.4 hr-1

u: growth rate of the cell (in K minimal medium, 2nd order)

0.275466667 hr-1

(exp. obtained)

Ψ: basal activity of the promoter

0.0017

kdeg,GFP,mRNA: GFP mRNA degradation rate (1st order)

0.0048s-1

=17.28 /hr

γI: Immature GFP degradation rate

0.7 hr-1

γm: Mature GFP degradation rate

0.021 hr-1

ρ: Immature GFP synthesis rate (trans rate, 1st order)

1440 protein/hr/mRNA (0.4protein/s/mRNA)

w: GFP maturation rate (1st order)

6.48 hr-1

Table 1. Parameters list 1.

Parameters

Values

ADP concentration

0.305 mM

ATP concentration

3.05 mM

Initial concentration of mRNA (GFP)

0/cell

Concentration of KdpF

10 µM

Concentration of KdpE

2.6 µM

Concentration of KdpD

2.6 µM

Concentration of inserted plasmid

0.0183 µM

Initial concentration of immature and mature GFP

0 /cell

Table 2. Parameters list 2.

Variables

Denotation

Unit

Concentration of KdpD

µM

Concentration of phosphorylated KdpD

µM

Concentration of phosphorylated KdpE

µM

Concentration of ADP molecules

mM

Concentration of ATP molecules

mM

Concentration of KdpF

µM

Rate constant of dephosphorylation of KdpE

 

 

 

Concentration of potassium ions

mM

Concentration of KdpE bound promoter PkdpF

µM

Concentration of Unbound promoter PkdpF

µM

Total concentration of promoter PkdpF

µM

Concentration of mRNA molecules for green fluorescence protein

 

Concentration of immature green fluorescent protein

µM

Concentration of mature green fluorescent protein

µM

Table 3. Variables list.


Reference:


Heermann R, Zigann K, Gayer S, Rodriguez-Fernandez M, Banga JR, et al. (2014) Dynamics of an Interactive Network Composed of a Bacterial Two- Component System, a Transporter and K+ as Mediator. PLoS ONE 9(2): e89671. doi:10.1371/journal.pone.0089671

Brewster RC, Jones DL, Phillips R (2012) Tuning Promoter Strength through RNA Polymerase Binding Site Design in Escherichia coli. PLoS Comput Biol 8(12): e1002811. doi:10.1371/journal.pcbi.1002811

Modeling, Simulation and Identification of the Dynamics of K Uptake in E. coli. (2014). Universitätsbibliothek der TU München.

Kelly, Jason et al. “Measuring the activity of BioBrick promoters using an in vivo reference standard.” Journal of Biological Engineering 3.1 (2009): 4.

J. Gayer, Stefan. "Modeling, Simulation and Identification of the Dynamics of K Uptake in E. Coli." Technische Universität München Fachgebiet Für Systembiotechnologie (2013). Print.

Kremling, A., Heermann, R., Centler, F., & Gilles, E. (2004). Analysis of two-component signal transduction by mathematical modeling using the KdpD/KdpE system of Escherichia coli.

Conboy, C., & Braff, J. (2013, May 29). Molecules of Equivalent GFP. Retrieved from http://openwetware.org/wiki/MEG