Team:HKUST-Rice/Safety

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SAFETY

SAFE ORGANISM

Species name (including strain)	Risk Group	Source	Risks to human	Ways for acquiring it	Ways of usage
E.coli (DH10B)	Group 1	http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf 013/500015_MTR-NALT_EN.pdf	It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.	We acquired from Professor King Lau Chow's lab in HKUST	As a chassis for carryout experiment, as a chassis for molecular cloning
E.coli (TK2240)	Group 1	National Institute of Health	It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.	Laermann, V., Ćudić, E., Kipschull, K., Zimmann, P., & Altendorf, K. (2013). The sensor kinase KdpD of Escherichia coli senses external K+. Molecular microbiology, 88(6), 1194-	As a chassis for carryout experiment related to PkdpF promoter and its mutant
E.coli (BL21 Rosetta)	Group 1	National Institute of Health	It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.	Acquired from Professor Minjie Zhang's lab in HKUST	For producing Crude cell extract in For TX-TL cell free system
E.coli (S17)	Group 1	U.S. Public Health Service Guidelines	It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.	Acquired from Silberg Lab in Rice University	E. coli used to conjugate with soil bacteria
E.coli (XL-1 EC)	Group 1	U.S. Public Health Service Guidelines	It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.	Acquired from Silberg Lab in Rice University	Standard strain used for cloning
E.coli (MG1655)	Group 1	U.S. Public Health Service Guidelines	It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.	Acquired from Silberg Lab in Rice University	WT used to conjugate w/ MG1655
E.coli (NEB 10-beta DH10B derivative)	Group 1	U.S. Public Health Service Guidelines	It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.	Acquired from Tabor Lab in Rice University	Standard strain used for cloning
E.coli (JW1212-1)	Group 1	U.S. Public Health Service Guidelines	It may be harmful to people who inhale or swallow. In rare case, such as with susceptible person, it can cause eye and skin irritation.	Acquired from Yale CGSC	As a chassis for carryout experiment related to PdusC promoters
Azotobacter vinelandii (ATCC 12837)	Group 1	http://www.agrian. com/pdfs/HYTa_0-0-0.5_MSDS.pdf	It may be cause eye irritation; discomfort and temporary nausea if swallowed accidentally.	ATCC	conjugation of E. coli to soil bacteria
Rhizobium leguminosarum (ATCC 10004)	Group 1	http://xitebio.ca/pea-&-lentil-inoculant/ PeasRhizo-MSDS.pdf	There is no known health effects.	ATCC	conjugation of E. coli to soil bacteria

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SAFE PROJECT DESIGN

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SAFETY TRAINING COURSES

This year's HKUST-Rice team members have received safety training over the summer. Topics that we have learned about in safety training are as following:

For HKUST:
1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management

The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to: http://www.ab.ust.hk/hseo/training.htm

For Rice University:
1. General Laboratory Safety
2. Blood Safety and Blood Borne Pathogens

The link to the laboratory safety training requirements of our institution (Rice University) can be directed to: https://osr.rice.edu/Content.aspx?id=788