Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and
research findings. The notebook for Project Pneuumonsensor and Project Riboregulator are displayed separately.
Pneumosensor
June 2014
Week 1
June 9
Wet lab
· Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600
· Transformation of BBa_B0034 and BBa_E0040 into DH10B
June 10
Wet lab
· Found reference promoter J23101 in BBa_I20260 from part registry
· Looked for Pcombox gene sequence from the genomic DNA
· Bacterial inoculation of BBa_B0034 and BBa_E0040